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ORIGINAL ARTICLE
Introduction
Centronuclear myopathy (CNM), also called myotubular
myopathy, is a congenital disease characterised by
centrally placed nuclei and generalised muscle weakness.1 CNM exists in X-linked recessive, autosomalrecessive and autosomal-dominant forms. The severity
From the Department of Clinical Sciences, College of Veterinary Medicine (MM-F, MDP, LC, RDG), and Cornell University Hospital for Animals, College of Veterinary
Medicine, Cornell University, Ithaca, New York, USA (MR, EAT)
Correspondence to Manuel Martin-Flores, DVM, DACVAA, College of Veterinary Medicine, Cornell University, Ithaca, NY, 14853 USA
E-mail: martinflores@cornell.edu
0265-0215 Copyright 2015 European Society of Anaesthesiology. All rights reserved.
DOI:10.1097/EJA.0000000000000222
potassium concentration before and after SCh administration were compared within groups with the paired
t-test. The increase in K concentration relative to baseline was compared between groups with the two-sample
t-test. The sensitivity (gain) of the AMG monitor and all
recovery variables [return of single twitch to 25, 75 and
90% of the final single twitch height and recovery index
(interval between ST 25 and 75%)] were compared
between groups with two-sample t-tests. Differences
were considered significant when P value was less than
0.05. All parametric data are summarised as mean (SD).
Descriptive statistics [nonparametric distribution;
median (IQR)] for electrolytes other than K, acidbase
variables and glucose before and after SCh administration
are also presented.
Results
All dogs recovered uneventfully from general anaesthesia. A transient decrease in arterial blood pressure of at
least 20% was observed in two CNM dogs following SCh.
These changes were self-limiting and required no intervention.
Onset time was 1.4 (0.4) min for CNM and 1.7 (0.6) min
for control dogs (P 0.47). Times to 25, 75 and 90%
recovery were significantly longer (P 0.001) for CNM
dogs than for controls (Fig. 2). The recovery index
was not significantly different between the treatment
groups [CNM 8.3 (3.5) vs. control 3.9 (2.1) min; P 0.15].
Performance of acceleromyography
Discussion
Our results show that the increase in K after SCh was
similar for the two groups of dogs. No electrocardiographic signs consistent with hyperkalaemia, such as tall
T waves, absence of P waves or wide QRS complexes,
were observed at any time.20 In two CNM dogs, we
observed a transient decrease in arterial blood pressure
after SCh, which resolved spontaneously. This might
have been due to histamine release, but no other signs
Fig. 1
K+ (mmol L1)
Control
CNM
5.0
5.0
4.5
4.5
4.0
4.0
3.5
3.5
3.0
3.0
2.5
2.5
Pre
Post
Pre
Post
Blood potassium concentration before and after succinylcholine 0.3 mg kg1 in dogs with autosomal-recessive centronuclear myopathy and controls.
Significant increase from baseline after succinylcholine; P < 0.05. However, the increase was similar between groups.
Table 1 Median (interquartile range) concentration of electrolytes, acidbase status and glucose concentration measured 5 min before (Pre)
and 5 min after (Post) succinylcholine was given to centronuclear myopathy and control dogs
CNM
Control
Pre
pH
SBE (mmol l1)
Na (mmol l1)
iCa2 (mmol l1)
Glucose (mg dl1)
7.39
1
142.5
1.29
122
Post
(0.03)
(1.5)
(1.75)
(0.11)
(26)
7.39
0
142.5
1.3
118.5
(0.04)
(3.25)
(4.75)
(0.09)
(36)
Pre
7.3
2.5
143.5
1.38
96
(0.04)
(2)
(3.5)
(0.07)
(18.5)
Post
7.3
3
141.5
1.36
106.5
(0.02)
(2)
(5.25)
(0.03)
(11.5)
Normal
7.35 to 7.45
5 to 0
139 to 150
1.12 to 1.4
60115
iCa2, ionized calcium; CNM, centronuclear myopathy; SBE, standard base excess.
100
CNM
Control
ST (%)
80
60
40
20
0
0
10
20
30
40
50
Time (minutes)
Spontaneous recovery of the single twitch (ST) to 25, 75 and 90%
(normalised to final single twitch value) after succinylcholine
0.3 mg kg1 in dogs with autosomal-recessive centronuclear myopathy
(CNM) and controls. Significant difference between groups; P < 0.05.
Fig. 3
Control
CNM
100
ST (%)
ST (%)
100
50
50
No visible twitch
0
0
10
20
30
40
Time (minutes)
10
20
30
40
Time (minutes)
Examples of the single twitch (ST) height after succinylcholine 0.3 mg kg1 (time zero) in a control dog and one with centronuclear myopathy (CNM).
In the CNM dog, twitch heights of 20% continued to be displayed by the monitor despite the absence of any observable evoked twitch.
between greyhounds and mixed breed dogs, no differences were observed.29 It appears that the differences in
recovery times that we observed between control and
CNM dogs are relatively benign, especially if the extent
of neuromuscular blockade is being measured objectively.
Acceleromyographic monitoring in dogs with CNM
proved challenging. Several attempts were required
before calibration could be performed. This was not
the case in the control animals, nor has it been our
experience when using similar protocols in earlier work.
In one dog, calibration was not possible and the gain was
manually increased to its maximum. During calibration of
the AMG, the signal (gain) is amplified so that the
response can be set to 100%. It follows that small evoked
responses might require higher signal amplification.
When signal amplification is high, the potential for erroneous measurements arising from background noise, such
as movement from surgical table, increases. In dogs with
CNM, the sensitivity used by the AMG was significantly
higher than in the control group, indicating higher signal
amplification. In these dogs, erroneous results were
observed at the time of complete block; the AMG displayed twitch heights of up to 20% when no visible or
palpable twitch could be detected (Fig. 3). This observation suggests that AMG monitoring might be prone to
erroneous measurements whenever the evoked response is
very small (and signal amplification high), as is the case in
many patients with neuromuscular disease. Similar difficulties have been reported when calibrating an AMG
monitor on neonates and small infants and whether the
sensitivity of the AMG monitor is adequate for patients
producing small responses is in question.30 Presumably,
our experience of myopathic dogs represented an exaggeration of that observation.
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Presentation: none.
References
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