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HTR_UMM
Pendahuluan
Penyakit Autoimmune terjadi dari kerusakan
dalam tubuh dengan adanya autoantibodies
atau autoreactive cells
Sekitar 2% populasi terkena penyakit ini
Terjadinya self tolerance pada individu ini
Self tolerance merupakan mekanisme clonal
deletion dari sel efektor, pengaturan aktif oleh sel
T dan pengaturan melalui jalur idiopatik
Umumnya sel T yang diproses memalui Thymus
tidak bertahan dan self reactive T cells
dihancurkan
Kehilangan sel T autoAbs
Pendahuluan
Terjadi situasi dimana antibody diproduksi
untuk melawan antibody itu sendiri
Ketika antibody diproduksi pertama kalinya,
area tersebut dikenali sebagai benda asing
oleh host
Kemudian host akan memproduksi antibody
untuk melawannya
anti-idiotypic Ab mungkin memiliki reaksi
melawan self Ags
Pendahuluan
Anti-idiotypic Abs ditemukan pada penyakit
seperti myasthenia gravis, diabetes mellitus, and
Graves disease
Kerusakan pada NK sel, dalam sekresi interleukin,
dalam phagocytosis dan complements dapat
terjadi
Hormon khususnya estrogen ditemukan untuk
meningkatkan aktivasi sel B dan menekan
pengaturan aktivitas sel T
Systemic Lupus
Erythematosus
Clinical Signs:
The first signs to appear are usually nonspecific such
as fatigue, weight loss, malaise, fever, and anorexia
Joint involvement of small joints of the hands, wrist
and knees is reported in over 90% of cases
A skin manifestation of an erythematous rash may
appear in the area of body exposed to UV light
A classic butterfly rash across the nose and cheeks
which is responsible for the name lupus may appear
Some patients may also exhibit renal involvement
Rheumatoid Arthritis
Rheumatoid arthritis is a systemic autoimmune
disorder
It involves the synovial membrane of multiple joints
Women are more likely to be affected than men
and usually strikes between the ages of 20 and 40
Rheumatoid Arthritis
Clinical Signs:
Symptoms include morning stiffness around the
joints lasting at least 1 hour, swelling of the soft
tissue around three or more joints
Others include swelling of the proximal
interphalangeal, metacarpophalangeal, or
wrist joints, symetric arthritis, subcutaneous
nodules, a positive RF test
Also included is a radiographic evidence of
erosion of the joints of the hands, the wrist, or
both
Rheumatoid Arthritis
Usually begins with nonspecific symptoms such as
fever, malaise, weight loss, and transient joint pain
Stiffness and joint pain that gradually improves
during the day are characteristics exhibited by most
patients
Joint are involved progressively to larger joints in a
symmetric manner from the knees, hips, elbows,
shoulders and cervical spine
About 25% of patients have nodules over the bones
Nodules may also be found in the myocardium,
pericardium, heart valve, pleural, lungs, spleen, and
larynx
Rheumatoid Arthritis
Immunologic Findings:
The main immunologic finding is the presence
of RF
It has been suggested that RF may be antiidiotypic antibodies involved in the regulation
of immune response
In RA, polyclonal activation of B cells may
occur resulting in overwhelming amount of
antibody to IgG
Rheumatoid Arthritis
These Abs may cause immune complex
formation with the activation of complement
which contribute to pathogenesis
Joint damage is due to invasion of inflammatory
cells such as neutrophils, and macrophages
Proliferation of fibroblast, macrophages, mast
cells, and stellar cells result in the formation of a
pannus, an organized mass of cells that grow
into the joint space
Rheumatoid Arthritis
Treatment:
Treatment include palliatives with rest and
nonsteroidal anti-inflammatory drugs like salicylates
and ibuprofen
Slow-acting antirheumatic drugs (SAARDS) may
also be used to treat the condition
New therapy include the use of monoclonal Abs
that target T cells
Hashimotos Thyroiditis
Hashimotos thyroiditis and Graves disease are
organ specific autoimmune diseases
Both diseases interfere with the thyroid gland
function
The thyroid gland located in the anterior region of
the neck consist of units called follicles
Follicles are lined with cuboidal epithelial cells and
filled with colloid
The primary constituent of colloid is thyroglobulin
which is made up of triiodothyronine (T3) thyroxine
(T4)
TRH acts on the pituitary gland to induce the release
of TSH
Hashimotos Thyroiditis
TSH binds to receptors on the cell membrane of the
thyroid gland causing break down of thyroglobulin
into T3 and T4
AutoAbs may interfere with this process and cause
under or overactivity of the thyroid
Hashimotos thyroditis is most often seen in women
between the ages of 30 and 40 years
Patients develop a combination of goiter or
enlarged thyroid, hypothyroidism and thyroid
autoantibodies
An association with HLA antigens DR4 and DR5 has
been noted. DQA1 and DQB1 genes seem to
confer resistance
Hashimotos Thyroiditis
Immunologic Findings:
Lymphocytic infiltration is seen with
development of germinal centers that almost
replace the normal glandular architecture of
the thyroid
Cell infiltrates include T and B cells,
macrophages and plasma cells
Both CD4 and CD8 cells are found thus the
disease is characterized by a cellular and a
humoral response
Autoantibodies are found in up to 80% of cases
Hashimotos Thyroiditis
Laboratory Testing:
The autoantibodies present include Abs to
thyroglobulin and to thyroid microsomal antigen
now known as thyroid peroxidase
Hashimotos Thyroiditis
Others are thyroid stimulating immunoglobulin (TSI)
and thyroid growth-stimulating immunoglobulin
(TGSI)
Peroxidase Abs can be measured by particle
agglutination assays, complement fixation, RIA and
Indirect IFA
Abs to thyroglobulin can be measured by
precipitaion in agar, indirect IFA, passive
agglutination, RIA, and EIA
Indirect IFA use human or monkey thyroid tissue
fixed to a slide
Antithyroglobulin Abs are found in about 80% of
patients with the disease
Graves Disease
Graves disease is another autoimmune
disease that affects the thyroid gland
Graves disease produces hyperthyroidism
It the most common cause of hyperthyroidism
and affects about 0.5% of the population
Women are more susceptible than men by a
margin of 7:1 and usually present between the
ages of 30 and 40.
In whites, the disease is associated with the HLA
antigen DR3 while in Asians HLA Bw35 and
Bw36 occur more frequently
Graves Disease
HLA DQB1 appears to confer resistance to the
disease
Clinical Signs:
Disease is presented as thyrotoxicosis with a
diffusely enlarged goiter that is soft instead of
rubbery
Signs include nervousness, insomnia,
depression, weight loss, heat intolerance,
sweating, rapid heart beat
Other signs include fatigue, cardiac
dysrhythmias, restlessness, and exopthalmus
Graves Disease
Immunologic Findings:
The thyroid presents with Hyperplasia with a patchy
infiltration of lymphocytes
Both CD4 and CD8 cells are present and the T cells
appear to play a central role in the pathogenesis of
the disease
The most significant Ab present is thyroid stimulating
hormone receptor antibody (TRab)
Ag-Ab combination result in the stimulation of the
receptor resulting in the release of the thyroid
hormones
Another group of Abs called thyroid stimulating
antibodies or immunoglobulins (TSab or TSI) may
have different specificity
Graves Disease
Laboratory Diagnosis:
A key finding in Graves disease is elevated levels of
total and free T3 and T4
In addition, TSH levels are low due to Ab stimulation
of the thyroid
Measurement of the thyroid Abs may be
undertaken if the above assays are unclear
Treatment:
Antithyroid medication may be employed.
Radioiodine which emits beta particles may be
used. Surgery is also an option.
Insulin-Dependent Diabetes
This disease is characterized by insufficient
production of insulin due to an autoimmune
destruction of the beta cells of the pancreas
Peak onset is b/w 10 and 14 years of age
Insulin-Dependent Diabetes
Viral infections have been linked with diabetes
Mumps virus, rubella virus, CMV, and Coxsakie B4
virus have all been inconclusively linked to diabetes
Congenital rubella infection is the only one for
which a link has been definitively identified
There appears to be similarity between coxsakie
viral protein P2-C and the enzyme glutamic acid
decarboxylase.
Antibodies are formed against glutamic acid
decarboxylase in IDDM
Molecular mimicry could initiate Ab production
against self Ag
Insulin-Dependent Diabetes
Immunopathology:
Inflammation of the islets of Langerhans in the
pancreas leads to fibrosis and destruction of
most of the beta cells
CD4 and CD8 B cells and macrophages are all
involved in the destructive process of the islet
cells
Cellular and humoral immunity are involved in
this process
The subset of T cells that is activated determines
whether the response is cellular or humoral
Insulin-Dependent Diabetes
Laboratory Testing:
IDDM is usually diagnosed by the presence of
hyperglycemia
Abs to islet cells may be screened for by
indirect IFA with human or rat islet cells
Islet cell may be detected in the sera of newly
diagnosed diabetic cases
Insulin-Dependent Diabetes
Treatment:
Insulin has been the standard form of
treatment
New treatment methods center around the use
of immunosuppressive agents
Agents that have been tried include
azathioprine, cyclosporine A and prednisone
Multiple Sclerosis
Destruction of the myelin sheath results in the
formation of lesions known as plaques in the white
matter of the brain and spinal cord
Multiple Sclerosis
90% of patients alternate between remissions and
relapses for many years
Within the plaques, CD4 cells, plasma cells and
macrophages are found along with immunoglobulin
Myasthenia Gravis
Symptoms of disease include facial weakness,
difficulty in chewing, and swallowing, difficulty
breathing
Others include inability to maintain support of the
trunk, the neck or the head
Antibody mediated damage to the acetylcholine
receptors in the skeletal muscle leads to muscle
weakness
May be associated with the presence of other
autoimmune diseases such as SLE
Appears to be linked with either HLA-B8 or DRw3
antigens
Myasthenia Gravis
80 to 90% of patients have Abs to acetylcholine
receptors which may be the main contributor
to pathogenesis
Combination of the Abs to the receptors blocks
the binding of acetylcholine which destroy the
receptors
Abs can be detected with RIA methods
Goodpastures Syndrome
Goodpastures syndrome is a
glomerulonephritis due to Abs reacting
specifically with Ags in the kidney
Necrosis of the glomerulus is triggered by an
Ab that reacts with glycoprotein present in
the basement membrane of the glomerulus
Results in immune complex deposit and
complement fixation which causes the
damage to the kidney
This may eventually produce renal failure