Tittle

: prove the elements that exist in proteins

Purpose

: students can prove the elements of what is in the protein

Observed data in table

No

Activity

Result of Observed
Before
Albumin : colorless

After
1. Albumin heated
Albumin,heated
Smell : like burn hair
Color : white
Vapour : have vapour (+++)
Sediment : have white
sediment
(base)
2. Albumin + NaOH Albumin : colorless
Albumin+NaOH,heated
heated
NaOH : colorless
Smell : like burn hair
Color : yellow wisk
Vapour : have vapour (+)
Sediment : have brown
sediment
(base)
3. Tofu extract
Tofu extract : white
Tofu extract+NaOH,heated
+NaOH heated
NaOH : colorless
Smell : like burn hair
Color : colorless
Vapour : have vapour (++)
Sediment : have white
sediment
(acid)
Conclusion : of the experiment has been done that can be concluded that albumin
heated,albumin+NaOH heated,and tofu extract contain nitrogen and carbon.

Tittle

: solubility albumin

Purpose

: students can prove the solubility of albumin to various solvents

Observed data in table

No

Activity

Result of Observed

After
Albumin + aquades =
colorless
Albumin + aquades,vortex =
colorless (have white lump)
2. Albumin + NaOH Albumin : colorless
Albumin + NaOH = yellow
0,2%,vortex
NaOH : colorless
wisk
Albumin + NaOH,vortex =
colorless
3. Albumin + HCl
Albumin : colorless
Albumin + HCl = colorless
0,2%,vortex
HCl : colorless
Albumin + HCl,votex =
colorless (have white lump)
4. Albumin +
Albumin : colorless
Albumin + NaCO3 = colorless
NaCO3
NaCO3 : colorless
Albumin + NaCO3,vortex =
0,2%,vortex
colorless
5. Tofu extract +
Tofu extract : white
Tofu extract + aquades =
aquades
Aquades : colorless
white
Tofu extract + aquades,vortex
= white
6. Tofu extract +
Tofu extract : white
Tofu extract + NaOH = white
NaOH 0,2%
NaOH : colorless
Tofu extract + NaOH,vortex =
white
7. Tofu extract +
Tofu extract : white
Tofu extract + HCl = white
HCl 0,2%
HCl : colorless
Tofu extract + HCl,vortex =
white (have white lump)
8. Tofu extract +
Tofu extract : white
Tofu extract + NaCO3= white
NaCO3 0,2%
NaCO3 : colorless
Tofu extract + NaCO3,vortex
=white(have white lump)
Conclusion : of the experiment has been done that can be concluded that albumin
soluable in aquades,base (NaOH),acid (HCl),and ansaturated salt (NaCO3)
1.

Albumin +
aquades,vortex

Before
Albumin : colorless
Aquades : colorless

Tittle

: Biuret test

Purpose

: students can demonstrate or prove the peptide bonds that form proteins

Observed data in table

No

Activity

Result of Observed

After
Albumin + NaOH =
colorless
Albumin + NaOH,vortex =
colorless
Albumin + NaOH,vortex +
CuSO4=purple (++)
2. 3 ml tofu extract +
Tofu extract : white
Tofu extract + NaOH =
1 ml NaOH 10% +
NaOH : colorless
yellow wisk
homogeneous with
CuSO4 : Blue
Tofu extract + NaOH,vortex
vortex + add 3
= white
drops CuSO4
Tofu extract + NaOH,vortex
+ CuSO4=purple (+)
Conclusion : of the experiment has been done that can be concluded that albumin
have a long peptide bond,and tofu extract have short peptide bond
1.

3 ml albumin + 1
ml NaOH 10% +
homogeneous with
vortex + add 3
drops CuSO4

Before
Albumin : colorless
NaOH : colorless
CuSO4 : Blue

Tittle

: Ninhidrin

Purpose

: Students can prove the existence of an amino acid

Observed data in table

No

Activity

Result of Observed
Before
Arginin : colorless
Ninhidrin : blue

After
1. Arginin + ninhidrin
Arginin + ninhidrin solution
solution,heated
= colorless
Arginin + ninhidrin
solution,heated = soft purple
(+)
2. Albumin + ninhidrin Albumin : colorless
Albumin + ninhidrin
solution,heated
Ninhidrin : blue
solution = colorless
Albumin + ninhidrin
solution,heated =purple (++)
3. Tofu extract +
Tofu extract :white
Tofu extract + ninhidrin
ninhidrin
Ninhidrin : blue
solution = colorless
solution,heated
Tofu extract + ninhidrin
solution,heated = white
Conclusion : of the experiment has been done that can be concluded that arginin and
albumin contain amino acid,but in tofu extract nothing happend