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  • Clinical Laboratory Science Review by Theriot (Part 3)

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    Gno Baguio
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    theriot Part 3

    Copyright:

    © All Rights Reserved
    Download as PDF or read online from Scribd
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    97 views4 pages

    Clinical Laboratory Science Review by Theriot (Part 3)

    Uploaded by

    Gno Baguio
    theriot Part 3

    Copyright:

    © All Rights Reserved
    Download as PDF or read online from Scribd
    Flag for inappropriate content
    of 4
    Zod wi adueyo w wy syusoa wonauny ogy > Yel ‘unop Suduyns eae Axoyeajdsoz ur wonounyshp Aaowiid y °g oqaey pur enauoyg {(parnsuadwoou) ae SISoPPY oyoquioyy Hametry svy worpsuaduoo penaed ‘aues perus0W yo amo [TNs st Fd agp Ng SUT poxH, so sfueyoots Sxovesuadwion WavUl ah 3] “p 4 pazanaoe sey uonwsusduioa (uy ‘peumgou st pd aq 3] og saplmexe Sapnorpoy ET Ea aif yo yous uy suns oseq-pioe ayp aurea een) SWaNGONd 35¥E-GIO ayn ats, FO 4 (Ssoimadsas) 7994 24 TY aH wen Buynes005 2054 5] senauy Bupesuoduron upeur ayy, “(Fujans) sqouciou, aroyp ov uonepes ui [ey Komp YOM ur aduoys # ur siznsos uopoung aaoya 998 09 £O9H Pue ZQDd ay Te yoo] “Zz, aqoqeiaw at Honounjecp 7.45. pH FCO, =55 HCOs = 24 Amvrer: Respirator pee ‘uncompensated); Phonetia and Paco are on the seesaw pth cP 38 ee pH =7.52 FCO, = 28 HCOg = 21 Answer: Partially compensated respiratory alkalosis. Phonetia and Paco are seesaw- ing. Carbo joins Paco to compensate Answer: Completely compensated metabolic acidosis or completely compensated respi- ratory alkalosis, For these situations, look at the pH. If it is on the low side of normal; choose acidosis, If it is on the high side of normal, choose alkaloris, Tn alike manner, completely compensated metabolic alkalosis cannot be distin. guished from fully compensitted respira tory acidosis, The Phrnetia, Paco and Carho story will work > 90% of the time in solving acid-base problems. Hemoglobin Derivatives ‘Hemoglobin (Hb) breakdown products include porphyrins, bilirubin and urobilinogen otonorphytin + Fert a heme b. Heme « globin = hemoglobin 2. Hb of aged or damaged RBCs converted to bilirubin LABORATORY ANALYSIS OF PORPHYRINS AND RELATED COMPOUNDS 1. Urines with large amounts of porphyrins show a red or 'port wine" color 2. All porphyrins have a characteristic pink fluorescence (cad be quantitated using a UY spectrophotometer) 3. Uroporphyrins can he extracted into n- butanol i 4, Watson-Sohwarte test a. Porphobilinogen (PBG) will reaet with Ehrlich's reagent, p-limethy- laminobenzaldehyde, to form red color b, Other substances also react ©. Control acidity and use chloroform or butanol to remove non-PBG color 4. Urobilinogen (UBC) will alse react with Ehrlich’s to form a red ealor ©. UBG is soluble in chloroform (top organic layer) £. PBC is not soluble in chloroform LABORATORY ANALYSIS OF BILIRUBIN, 1, Diazotization methods a. Evelyn & Malloy (classic method) Bilirubin + dieser sulfanilic acid = azobilirubin (purple) % Total bilirubin reacts with diazo reagent in methyl alcohol; reaction read in 30 minutes % ‘Conjugated bilirubin (direct) reacts with diazo reagent in water; reaction read in I minute b. Jendvassik-Grof method (same except the accelerator is caffeine-bensoate} © Direct = conjugated = water soluble 4. Indirect = unconjugated » insoluble in ‘water 2, Direct Spéctrophotometric method 8. Specimen collection and handling 4. Bilirubin is light sensitive, therefore sample should be stored in the dark ert spoyiau feouaypounmnay “7 TWwaanaa 6Buyoyuow Brug oyneddiey! pup ABojooIxoy omong (waSoupgait Gok nny? ¥ 10 5 a1049 o1oym sorsrooud aispoion 4oip0 wosf sioffip = ,yonedip,, uo potasou sp Sunodd9) NASONTUBONN XUVNTUN ON st 2x00 08 vizojoeq founsonandy wep ‘yong aq rouuvo ugramg porstin{uosmm ou st aout 08 ‘outm amp tt pafaiox9 aq vouuea (oqqnjosuy sarom) poreanfuoatt * _soyBu0au 305 yeuttow se aqrameg pareSafuod sy wuquanyq (pareSe{uoaun) ywompur unas g 'S0)0U08u Ut D=yoRN{tOD!* 6g jouuos ugning sous. i pexojco Aepp aurooag Kew poorg “2 eaSourjgoan on Mop weyouy og 07 aunsortN FuajU9 moa gna polos nesoad (aoyooy9 01 ap) uafourpqoan A 4 oT her de yood, oon poxgjoqeiout oq youn uiqayg paesnfuooun pu poreBnfuny -» soipuul (oamonssqo) anedatjiug “¢ woZouspyoan oui nna aun Ye wLqMagi porofafuos yy woriounys4p gay] 04 mp wafougyqoan y pur uxpasiqy poi afuos uqnamig pairinfeoon y ov (soya ‘sempdoy ouse 2°1) ooxpunel sn etloy - vofomyryorn aujan Ye wquayEG poresin{uosun y 9 xq ou paw uqnagy poresniue, TeuULoH — ayes [rutOR we sando8 ‘woneinfuoo ‘jeuaz0w st uorioung aoary “4, uuqnaygg paeatuosnin ¥ Stomp ne pa yw gnsqouy -t) vorpunef syedpoag sNowianoo suyan ut avadde Yppm i“ st (aqryos 4270) gna poyeBh(uos oxy, “NCH (

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