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Final Conference SecuFood Project

With the support of the Prevention,


Consequence Management of Terr
Security-related Risks Pro
European Commission - Directorate
Freedom and Securi

Technologies for Food Defence

Marcella Trombetta PhD


Full Professor
Chairman of Chemistry & Biomaterials Labs

Email: m.trombetta@unicampus.it
http:// www.unicampus.it

Auditorium Biagio DAl


Italian Ministry of the He

Rome, 27th April 20

Food Chain

With the support of the Prevention,


Consequence Management of Terro
Security-related Risks Prog
European Commission - DirectorateFreedom and Security

Schematic food chain of a simple hamburger ..


A COMPLEX SYSTEM !!!

Food Defence

With the support of the Prevention,


Consequence Management of Terro
Security-related Risks Prog
European Commission - DirectorateFreedom and Security

The term to encompass activities associated with protecting t


Nations food supply from deliberate or intentional acts
contamination or tampering
U.S. Department of Homeland Security 2003:
the food and agriculture sector is one of the 17 critical national
infrastructures open to intentional attack

Everyone must eat!

With the support of the Prevention,


Consequence Management of Terro
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European Commission - DirectorateFreedom and Security

To this foundational aspect of o


everyday life have the potential
cause a great deal of harm:
Physical: depending on the contaminant or where its used,
people or livestock could sicken or even die.
Economic: medical cost, quarantines, containment of food produ
decontamination and disposal of carcasses or product
Physiological: loss of Consumers confidence
Political: political unrest

Agro- terrorism

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Consequence Management of Terro
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European Commission - DirectorateFreedom and Security

 Intelligence: food sector is a terrorist target


 Food and agriculture are critical assets (> 1 trillion
EURO in 2001
 Source of major jobs (farm fast food chain)
 Boundary around the food chain is porous

Terrorism
Contamination with CBRN agents
Chemical, Biological (Bioterrorism), Radiological, and
Nuclear
In Europe the food supply chain, although
identified as a critical sector, has received
comparatively less attention in terms of initiatives
to reduce potential consequences of deliberate
attacks.
Although excellence elements can be
identified, specific EU prevention mechanisms
are missing!

With the support of the Prevention,


Consequence Management of Terro
Security-related Risks Prog
European Commission - DirectorateFreedom and Security

Terrorist attacks
Food Supply
Chain

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Consequence Management of Terro
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European Commission - DirectorateFreedom and Security

Terrorist attacks
aiming to create lack of trust and
spread panic in developed countries
Multi-disciplinary topic






low enforcements,
food safety agencies,
health organisations,
food firms,
etc.

This limited problem-sharing capability at


EU level !

Food Defence Plan


 Assess
your
vulnerabilities
to
contamination

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Consequence Management of Terro
Security-related Risks Prog
European Commission - DirectorateFreedom and Security

operatio
intention

Write a food defence pl


detailing countermeasures
reduce the risk of intention
contamination

Prepare e response plan


fast, efficient containment of
emergency

 Manage your food defence pl


for the long term

Food Defence Plan

With the support of the Prevention,


Consequence Management of Terro
Security-related Risks Prog
European Commission - DirectorateFreedom and Security

What are the benefits of developing a food defense plan


Having a food defense plan for your operation can reduce the ri
of intentional contamination to your operation and may ultimate
benefit your bottom line.

 Prevention
 Response
 Recovery

With the support of the Prevention,


Consequence Management of Terro
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European Commission - DirectorateFreedom and Security

Who do Consumers believe?

Who do Consumers believe


is responsible for Food
Defence?
Consumers
11%
Retailers 10%

Government
31%

Transporters
and
Distributors
9%

Who do Consumers believ


is responsible for paying fo
Food Defence?
Consumers 8%
Retailers 12%

Government
30%

Transporters
and
Distributors
13%

Manufacturers
and
Processors
27%

Farmers 12%

Source: Stinsons et al. (2008)

Manufacturers
and
Processors
22%

Farmers

Food tampering

With the support of the Prevention,


Consequence Management of Terro
Security-related Risks Prog
European Commission - DirectorateFreedom and Security

the intentional contamination of a food product,


with the intent to cause harm to the consumer or t
a private company
Refers to anything that may affect any part of the fo
product, such as the product itself, or it can affect t
packaging and the label
It is a punishable offence under the Criminal Code of almo
all the Member States of the European Commission

Reasons of Food Tampering


 trying to draw attention to a "cause"  revenge, by causing financial lo
by getting free publicity;
or ruining a company's reputatio
 extortion for personal gain;
 reasons known only to t
 mischief or prank;
perpetrator.
 terrorism;

Signs of tampering

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 packaging that has been opened and resealed;


 products that have damaged or missing safety seals or tampe
evident seals;
 products or packaging that is cut, torn, punctured
discoloured;
 products that are dirty or damaged;
 products with strange odour or flavour;
 cans or jars with signs of leakage, spillage or corrosion;
 vacuum-packed products with no vacuum seal;
 packaging that has been altered, including labels, product l
codes, and other identifying information; and
 the presence of a foreign object or non-food item in the product

Food tampering

With the support of the Prevention,


Consequence Management of Terro
Security-related Risks Prog
European Commission - DirectorateFreedom and Security

Consequence of food tampering:

















expenses linked to laboratorys analysis;


recall and stock expenses;
management costs linked to the merchandise cycle;
legal expenses;
compensations;
insurance costs;
communication costs;
possible expenses due to the final or temporary closure of commercial
markets and/or logistic or production structures;
selling off costs;
decreasing of purchases;
mid-term effect on the consumers trust;
costs connected to the relaunch of the product and/or of the industry;
decreasing of economic flow;
missed cash in.

Food Defence

With the support of the Prevention,


Consequence Management of Terro
Security-related Risks Prog
European Commission - DirectorateFreedom and Security

Technologies for Food Defence


1) Food traceability
ICT Systems for Food Traceability The Italian Case
RFID technology
2) Automatic Control Techniques for Food Supply Chain
Digital Image Processing Techniques to Improve Food Security
3) Nano-Biosensors for food defence
Polymeric nanowires-Ab sensors
Nano-BEAM-Ab sensors
Nano-BEAM-aptamer sensors
Nano-BEAM-DNA-sensors
Bio-barcode DNA sensors

Food Defence

With the support of the Prevention,


Consequence Management of Terro
Security-related Risks Prog
European Commission - DirectorateFreedom and Security

Technologies for Food Defence


1) Food traceability
ICT Systems for Food Traceability The Italian Case
RFID technology
2) Automatic Control Techniques for Food Supply Chain
Digital Image Processing Techniques to Improve Food Security
3) Nano-Biosensors for food defence
Polymeric nanowires-Ab sensors
Nano-BEAM-Ab sensors
Nano-BEAM-aptamer sensors
Nano-BEAM-DNA-sensors
Bio-barcode DNA sensors

Food Traceability

With the support of the Prevention,


Consequence Management of Terro
Security-related Risks Prog
European Commission - DirectorateFreedom and Security

TRACEABILITY
refers to the completeness of the information abou
every step in a process chain
 In food processing refers to the recording of all movement of product a
steps within the production process
 Where traceability has been closely adhered to, it is possible to identify,
precise date/time and exact location which goods must be recalled, a
which are safe, potentially saving millions of dollars in the recall process
 Traceability within the food processing industry is also utilized to iden
key high production & quality areas of a business, versus those of l
return, and where points in the production process may be improved

Food Traceability

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Consequence Management of Terro
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European Commission - DirectorateFreedom and Security

Traceability systems imply the use of a unique piece of data which can
traced through the entire production flow, linking all sections of the busine
including suppliers and future sales through the supply chain

The use of a barcode / RFID


RFID: Radio-frequency identification is the use of an object - typically refer
to as an RFID tag - applied to or incorporated into a product, animal, or per
for the purpose of identification and tracking using radio waves. Some tags
be read from several meters away and beyond the line of sight of the reader
A
barcode
is
an
optical
machine-readable
representation of data, which shows certain data on
certain products. Originally, barcodes represented
data in the widths (lines) and the spacings of parallel
lines, and may be referred to as linear or 1D (1
dimensional) barcodes or symbologies

Food Traceability

With the support of the Prevention,


Consequence Management of Terro
Security-related Risks Prog
European Commission - DirectorateFreedom and Security

The European Union's General Food Law


It came into force in 2002, making traceability compulsory for food and fe
operators and requiring those businesses to implement traceability system
(http://ec.europa.eu/food/food/foodlaw/index_en.htm)
The EU introduced its Trade Control and Expert System, or TRACES,
April 2004. The system provides a central database to track movement
animals
within
the
EU
and
from
third
countri
(https://sanco.ec.europa.eu/traces/)
The new EC Regulation 178/2000 has a number of specific requirements f
enhanced traceability (EC, 2002)
Traceability facilitates trade or public recalls. Under the new EC regulatio
food business operators will have to have systems in place enabling them
identify immediate supplier(s) and immediate customer(s) of their products

Food Defence

With the support of the Prevention,


Consequence Management of Terro
Security-related Risks Prog
European Commission - DirectorateFreedom and Security

Technologies for Food Defence


1) Food traceability
ICT Systems for Food Traceability The Italian Case
RFID technology
2) Automatic Control Techniques for Food Supply Chain
Digital Image Processing Techniques to Improve Food Security
3) Nano-Biosensors for food defence
Polymeric nanowires-Ab sensors
Nano-BEAM-Ab sensors
Nano-BEAM-aptamer sensors
Nano-BEAM-DNA-sensors
Bio-barcode DNA sensors

Food Traceability

With the support of the Prevention,


Consequence Management of Terro
Security-related Risks Prog
European Commission - DirectorateFreedom and Security

ICT Systems for Food Traceability The Italian Case


 No standard computer at European level, for the
management of traceability
 Companies operating in partnership trying to improve
traceability systems of their own devising:
Vegetal Production Research Centre (CRPV- www.cprv.it )
Net-Agree (www.net-agree.com )
Computer supports for :
 a manual traceability;
 GUA and GIAS-PCsoftware for managing field data;
software able to collect data that are generated in the business of product
manufacturing (TRACKING);
internet interface for the client: www.rintracciabilita.it

Food Defence

With the support of the Prevention,


Consequence Management of Terro
Security-related Risks Prog
European Commission - DirectorateFreedom and Security

Technologies for Food Defence


1) Food traceability
ICT Systems for Food Traceability The Italian Case
RFID technology
2)Automatic Control Techniques for Food Supply Chain
Digital Image Processing Techniques to Improve Food Security
3) Nano-Biosensors for food defence
Polymeric nanowires-Ab sensors
Nano-BEAM-Ab sensors
Nano-BEAM-aptamer sensors
Nano-BEAM-DNA-sensors
Bio-barcode DNA sensors

With the support of the Prevention,


Consequence Management of Terro
Security-related Risks Prog
European Commission - DirectorateFreedom and Security

Food Traceability
RFID technology
Radio-Frequency IDentification is the use of a tag
applied to or incorporated into a product, animal, or
person for the purpose of identification and tracking
using radio waves
FIRST PART

SECOND PART

 integrated circuit for


storing and processing
information;
 modulating and
demodulating a radiofrequency (RF) signal, and
other specialised
functions

 antenna for receivin


and transmitting the
signal

Food Traceability

With the support of the Prevention,


Consequence Management of Terro
Security-related Risks Prog
European Commission - DirectorateFreedom and Security

RFID technology: types of RFID Tags


Active RFID Tag: contains a battery and can transmit signals
autonomously

Passive RFID Tag: has no battery and require an


external source to provoke signal transmission

Semi-Passive RFID Tag (BAP): battery assisted


passive which require an external source to wake
up but have significant higher forward link
capability providing great read range

Food Traceability

With the support of the Prevention,


Consequence Management of Terro
Security-related Risks Prog
European Commission - DirectorateFreedom and Security

RFID technology is used in enterprise supply chain management to


improve the efficiency of inventory tracking and management:
Product tracking
Transportation and logistics
Animal identification
Replacing barcodes

Food Defence

With the support of the Prevention,


Consequence Management of Terro
Security-related Risks Prog
European Commission - DirectorateFreedom and Security

Technologies for Food Defence


1) Food traceability
ICT Systems for Food Traceability The Italian Case
RFID technology
2) Automatic Control Techniques for Food Supply Chain
Digital Image Processing Techniques to Improve Food Security
3) Nano-Biosensors for food defence
Polymeric nanowires-Ab sensors
Nano-BEAM-Ab sensors
Nano-BEAM-aptamer sensors
Nano-BEAM-DNA-sensors
Bio-barcode DNA sensors

Automatic Control Techniques

With the support of the Prevention,


Consequence Management of Terro
Security-related Risks Prog
European Commission - DirectorateFreedom and Security

Automatic Control Techniques for Food Supply Chain


Methods based on closed-loops control algorithms, where controllers have
the following roles:
To compare the value of the measured variable with the set-point value
To calculate the error and on the basis of this, providing the control action
Digital Image Processing Techniques to Improve Food Security
The choice of product to be lead to the sales network in order to offer highe
quality and security. Advantages:
 greater objectivity in the choice of the product
 an improved control over possible counterfeits

Automatic Control Techniques


Digital Image Processing Techniques
to Improve Food Security
Image processing is divided into 5 phases:
Image Acquisition
Pre-processing
Segmentation
Measurements
Classification

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Consequence Management of Terro
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European Commission - DirectorateFreedom and Security

Digital Image Processing


Sensors for images acquisition:
CCD, Ultrasound, MRI, CT, ET
Rules of discrimination of image objects:
shape, size, color, texture,
All such information collected is useful for the final
classification is done with algorithms specific logic
for each food industry
The advantages of using this technique are:
Improvement of product quality and security, as it
eliminates the subjectivity of the operator choice
and inattention
Ability to perform tasks that require high precision
The selection criteria can be easily changed by
software

With the support of the Prevention,


Consequence Management of Terro
Security-related Risks Prog
European Commission - DirectorateFreedom and Security

Food Defence

With the support of the Prevention,


Consequence Management of Terro
Security-related Risks Prog
European Commission - DirectorateFreedom and Security

Technologies for Food Defence


1) Food traceability
ICT Systems for Food Traceability The Italian Case
RFID technology
2) Automatic Control Techniques for Food Supply Chain
Digital Image Processing Techniques to Improve Food Security
3) Nano-Biosensors for food defence
Polymeric nanowires-Ab sensors
Nano-BEAM-Ab sensors
Nano-BEAM-aptamer sensors
Nano-BEAM-DNA-sensors
Bio-barcode DNA sensors

With the support of the Prevention,


Consequence Management of Terro
Security-related Risks Prog
European Commission - DirectorateFreedom and Security

Nano-Biosensors
Nanostructured Biosensors

Better Prevention

Stronger Protection

Faster Response

Min contamination

Min public exposure

Recovery quicly

Safe Secure Food

 Protect the Public


 Less illness
 Reduce chance of successful attack

Biosensors

With the support of the Prevention,


Consequence Management of Terro
Security-related Risks Prog
European Commission - DirectorateFreedom and Security

Biosensors are analytical devices which are capable of providing eith


qualitative or quantitative results
Biosensors combine the requisite selectivity of biology with the processi
power of modern microelectronics and optoelectronics to offer powerful n
analytical tools with major applications in medicine, environmen
diagnostics and the food and processing industries
Biosensors consist of bio-recognition systems, typically enzymes or bindi
proteins, such as antibodies, immobilised onto the surface of physic
chemical transducers
Specific interactions between the target analyte and the complementa
biorecognition layer produces a physico-chemical change which is detect
and may be measured by the transducer. The transducer can take ma
forms depending upon the parameters being measured - electrochemic
optical, mass and thermal changes are the most common

Principle of Biosensor

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European Commission - DirectorateFreedom and Security

Biosensors Schematic

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Advantages:
Rapid detection time
High sensitivity and specificity
Compatible with data
processing technologies
Source: Lazcka et al. (2007)

Detection Mechanism

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Tested Samples
 Milk
 Fruits
 Strawberries
 Vegatables
 Alfalfa sprouts
 Lettuce
 Tomatoes
 Ground meat
 Cooked food
Fried rice
Corn

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Performance
Sensitivity and Selectivity

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Nano-BEAM

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Biologically enhanced electrically active magnetic nanoparticles


Bacillus Anthrac

100 l of
EAM
captured
spores!

Food Defence

With the support of the Prevention,


Consequence Management of Terro
Security-related Risks Prog
European Commission - DirectorateFreedom and Security

Schematic food chain of a simple hamburger ..


Technologies for Food Defence in the whole steps of the
COMPLEX SYSTEM !!!

With the support of the Prevention,


Consequence Management of Terro
Security-related Risks Prog
European Commission - DirectorateFreedom and Security

Thank you for


your attention!
m.trombetta@unicampus.it

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