TRUNG TM KIM NGHIM THUC, M PHM, THC PHM

THNH PH H CH MINH

BO CO THC T
TI PHNG KIM NGHIM THC PHM
(ngy 08/07/2016)

Nhm 4:
Trn Th Mai Thi

Trn Phng Trinh

Trn Th Thy

Nguyn Phan Th Trinh

Khng Minh Thng

Nguyn c Tri Thc

Thnh ph H Ch Minh
2016

1. NI DUNG THC TP
nh tnh h th trong ch phm H th hon; khon ng
hoa, tri mu trong ch phm Bo ph khang.

2. KHI QUT V PHNG PHP SC K LP MNG

2.1. Nguyn tc
Phng php sc k lp mng (SKLM) c dng nh tnh, th
tinh khit v i khi c dng thc hin bn nh lng hot
cht.
SKLM l k thut tch cc cht c tin hnh khi cho pha ng di
chuyn qua pha tnh trn t hn hp cc cht cn tch. Pha
tnh l cht hp ph c chn theo tng yu cu phn tch, c
tri thnh lp mng ng nht v c c nh trn cc phin knh
hoc phin kim loi. Pha ng l mt h dung mi n hoc a
thnh phn c trn vi nhau theo t l quy nh trong tng
chuyn lun. Trong qu trnh di chuyn qua lp hp ph, cc cu t
trong hn hp mu th di chuyn trn bn mng theo hng di
chuyn ca pha ng vi nhng tc khc nhau. Kt qu ta thu
c mt sc k trn bn mng. C ch phn tch c th l hp
ph, trao i ion, ry phan te hay s phi hp ng thi ca nhiu
c ch ty thuc vo tnh cht ca cht lm pha tnh v dung mi
pha ng.
i lng c trng cho mc di chuyn ca cht phn tch l h
s di chuyn Rf.
Rf= a/b
Trong :
a l khong cch di chuyn ca cht phn tch (cm)
b l khong cch di chuyn ca dung mi (cm)

3. THC NGHIM
3.1. nh tnh H th
i tng nghin cu: Thc phm chc nng H th hon (l
200 vin) ca C s sn xut thuc YHCT Bo Ngha vi thnh phn
h th ch 40 g, phc linh 1 g, ng quy 8 g, k t 8 g, th ty t
5 g, ph c ch 3 g, ngu tt 4 g, xuyn khung 3 g, bch thc 8 g,
thc a 3 g, mc hng 3 g, sinh khng 3 g.
Phng php sc k lp mng:
Bn mng: Silica gel G
Dung mi trin khai: Ethyl acetat methanol nc (100:17:13)
Dung dch th: Ly 0,25 g bt dc liu, them 20 ml ethanol 96%
(TT), un hi lu cch thy trong 30 pht, ngui, lc, bay hi
n cn kh. Cn them vo 10 ml nc v 1 ml dung dch acid HCl
10% (TT) un cch thy 30 pht, ngui, sau lc vi 20 ml
ether ethylic (TT) 2 ln, dch ether c bay hi cn khong 1 ml
dng lm dung dch th.
Dung dch i chiu: Ha tan emodin trong ethanol 96% (TT)
c dung dch c nng 0,1%. Nu khng c cht i chiu th
dng 0,25 g bt H th (mu chun), chit nh dung dch th.
Cch tin hnh: Chm ring bit ln bn mng 5 l dung dch th v
dung dch i chiu. Sau khi trin khai, kh bn mng trong
khng kh nhit phng, quan st cc vt di nh sng t ngoi
bc sng 365 nm v hin mu bng hi amoniac.
Trn sc k ca dung dch th phi c cc vt pht quang cng
gi tr Rf v mu sc vi vt t c trn sc k ca dung dch
i chiu nu dng dc liu chun chun b dung dch i chiu;
hoc trn sc k ca dung dch th phi c mt vt pht quang

c cng gi tr Rf v mu sc vi vt ca emodin trn sc k ca

dung dch i chiu.
3.2. nh tnh khon ng hoa
i tng nghin cu: Thc phm chc nng Bo ph khang ca
Cng ty c phn dc phm ng dc 5 vi thnh phn xuyn bi
mu 200 mg, t b dip 160 mg, tang bch b 160 mg, bch b 160
mg, khon ng hoa 100 mg, tri mu 100 mg, ng sm 100 mg,
hnh nhn 100 mg, thin mn 100 mg, bc h 80 mg, hong cm 50
mg, t uyn 50 mg, cam tho 50 mg.
Phng php sc k lp mng
Bn mng: Silica gel G
Dung mi khai trin: Cyclohexan ethyl acetat (9.5 : 0.5).
Dung dch th: Ly 2 g bt dc liu, thm 2 ml ether du ha (40 oC
60 oC) (TT), ngm v thnh thng lc trong 1h. Lc, bc hi t nhin
n cn. Ha cn trong 1 ml cloroform (TT).
Dung dch i chiu: Ly 2 g bt Khon ng hoa ( muc chun),
chit nh mu th.
Cch tin hnh: Chm ring bit ln bn mng 20 l mi dung dch
th v dung dch i chiu. Sau khi khai trin, bn mng c
kh ngoi khng kh hay sy nh cho bay ht dung mi. Soi di nh
sng t ngoi bc sng 365 nm sau phun dung dch Vanillin
Acid sulfuric (TT), sy 105 oC cho n khi xut hin vt.Trn sc k
ca dung dch th phi c cc vt c cng mu sc v gi tr Rf vi
cc vt ca dung dch i chiu.
3.3. nh tnh Tri mu
i tng nghin cu: Thc phm chc nng Bo ph khang ca
Cng ty c phn dc phm ng dc 5 vi thnh phn xuyn bi

mu 200 mg, t b dip 160 mg, tang bch b 160 mg, bch b 160
mg, khon ng hoa 100 mg, tri mu 100 mg, ng sm 100 mg,
hnh nhn 100 mg, thin mn 100 mg, bc h 80 mg, hong cm 50
mg, t uyn 50 mg, cam tho 50 mg.
Phng php sc k lp mng
Bn mng: Silica gel GF

254

Dung mi khai trin: Cloroform ethyl acetate (11:1)

Dung dch th: Ly 5 g bt dc liu, thm 50 ml ethanol 95 % (TT),
un hi lu 60 pht, ngui, lc, ly dch lc, thm 2 ml dung dch
acid sulfuric 5 % (TT), un hi lu trong cch thy 3h v sau c
dch ny n cn. Ha tan cn trong 2 ml cloroform (TT).
Dung dch i chiu: Ha tan sarsasapogenin chun trong cloroform
(TT) c dung dch cha 5 mg/ml lm dung dch i chiu. Nu
hng c sarsasapogenin, ly 5 g bt Tri mu (mu chun) ri chit
nh mu th.
Cch tin hnh: Chm ring bit ln bn mng 7 l mi dung dch
th v dung dch i chiu. Trin khai xong, ly bn mng ra phi
kh ngoi khng kh. Phun hn hp ca dung dch vanilin 8 % trong
ethanol khan (TT) v dug dch acid sulfuric (0.5:5) [pha dung dch
acid sulfuric: Ly 2 ml nc, thm cn thn 7 ml acid sulfuric (TT),
lm lnh, thm nc va 10 ml]. Sy bn mng 5 pht 100 oC.
Trn sc l ca dung dch th phi c cc vt cng mu sc v
gi tr Rf vi cc vt trn sc k ca dung dch i chiu.

4. KT QU