that means the micro-organisms present in water lives certain period of time whe re the Return activated MLSS consume F/M in aeration basin is about 23 days of t ime period, formula mlvss present in areation divided by outlet Mlvss multiply by available F/M in w aste water What will happen the after 23 days The microorignanis will die due to lack of food, Even producing the Oxygen in ba sin the dead organisms releasing bio nitrates that causes the remaining the live s to die. formulae