Table 3: Key Biochemical Tests for Differentiating Enterobacteriaceae

ORGANISM
Arizona hinshawii

TSI
KorA/A,g

Citrobacter freundii

AorK/A,g

Edwardsiella tarda

K/A,g

Enterobacter aerogenes

A/A,g

Escherichia coli

A/A,g

Klebsiella pneumoniae

A/A,g

Morganella morganii

K/A,g

Proteus mirabilis

K/A,g

Proteus vulgaris

KorA/A,g

TSI MIO
H2S IND MR V-P CIT URE

Providencia rettgeri

K/A,g+

Salmonella paratyphi B

K/A,g

+
+
+
+
+
+

K/A

KorA/A

Salmonella typhi
Serratia marcesens
Shigella sonnei

K/A

Other Shigella spp.

K/A

Yersinia enterocolitica

A/A

Alcaligenes faecalisNE

K/NC

Pseudomonas aeruginosa

NE

K/NC

+
+
+
+
+
+
-

+
+
+
+
+
+
+
+
+
+
+
+
+

+
+
+
+
-

+
+
+
+
w

+
+
+
+
+
+

MIO
MOT

+
+
+
+
+
+
+
+
+
+
+
+
-

+
+
+
+
+ - ;+
+
+
(35oC)

(25oC)

MIO
SUGAR FERMENTATION
ODC LDC GLU LAC SUC MAN
A
+ + A,g A,g -

+
+
+
+
+
+
+
+
+
-

+
+
+
+
+
+
+
-

A,g

A,g

A,g

A,g

A,g

A,g

A,g

A,g

A,g

A,g

A,g

A,g

A,g

A,g

A,g

A,g

A,g

A,g

A,g
A
A
A,g
A
A,g+
A+
A
A

A,g

A = acid; K = alkaline; NC = no change; g = gas; d = delayed; w = weak; NE = Non-Enterobacteriaceae (nonfermentative aerobes)