Separation of Resveratrol and Related Substances in

Dietary Supplements on the Acclaim 120 C18 Column

4000

Column:
Dimensions:
LC System:
Mobile Phases:

Gradient Time (min):

%A
%B
Flow Rate:
Temperature:
Injection:
Detection:

1
A
mAU B

Peaks:

1. Polydatin
2. Resveratrol
3. Pterostilbene

E
0

Acclaim 120 C18, 3 m

3.0 250 mm
UltiMate 3000 RSLC
A. Methanol
B. 0.02% H3PO4 (v/v)
-8
0 2
12 13
40
40 40
75 90
60
60 60
25 10
0.60 mL/min
25 C
2 L
UV at 310 nm
Spectral confirmation
200450 nm
R
H
H
CH3

R
glucose
H
CH3

OR
2

8
10
Minutes

Chromatograms:
A. Brand #1; 17 mg resveratrol
B. Brand #2; 25 mg total resveratrol
C. Brand #3; 16 mg total resveratrol
D. Resveratrol standard; 50 g/mL
E. Brand #4; <0.06 mg resveratrol

12

14

Sample Preparation:
1. Weigh contents of capsule
2. Take aliquot of 1/10
3. Add 1.0 mL 0.02% H3PO4
and 4.0 mL methanol
4. Sonicate 10 min then filter

17
HO
OR

26147

Buyer beware. When buying dietary supplements, the manufacturer may not fully disclose the content or form of the
active ingredient. In these examples, one vendor supplies resveratrol, two others supply mixtures of resveratrol and
polydatin, and one provides nothing at all. The superior resolution of the Acclaim C18 3 m 3.0 250 mm column easily
separates resveratrol from related compounds.

2009 Dionex Corporation. All rights reserved.