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ISSN: 2320-2246
ABSTRACT
Citronella grass has been serving from years as a mosquito repellent in the field of ancient and modern medicine.
Commercially available mosquito repellents are chemical based and disastrous to human health. An attempt has
been made to prepare a 100% herbal product based on Citronella leaf remains which is left out and of no use
after steam distillation. It is cheap, effective and environment friendly. It is a first and preliminary work based on
formulating and evaluating herbal mosquito repellent cakes using natural binders such as neem powder, potato
starch, corn starch, coconut shell powder, wood powder and cow dung. The efficacy of prepared citronella leaf
cakes were evaluated on three different parameters such as flammability, burning time and mosquito repellency
test. Also, the cakes were sprayed with different concentrations of Citronella oil. Based on the results obtained
from these parameters, the residual percentage of each combination of cakes was calculated and it suggested
that Neem powder cake has the most effective repellency activity when impregnated with 10% Citronella oil.
Keywords: Citronella leaf cakes; flammability; mosquito repellent; natural binders; residual percentage
INTRODUCTION
Controlling mosquitoes is of utmost importance in the
present day scenario with rising number of mosquito
borne diseases. An alarming increase in the range of
mosquitoes is mainly due to deforestation,
industrialized farming and stagnant water. Thus,
special products like mosquito repellents for combating
mosquitoes are required. The products used for
mosquito control have varying degrees of effectiveness.
Carbon dioxide and lactic acid present in sweat in
warm-blooded animals act as an attractive substance
for mosquitoes. The perception of the odor is through
chemoreceptors present in the antennae of mosquitoes.
Insect repellents work by masking human scent. A
number of natural and chemical mosquito repellents
were studied in many research papers and review
papers [3, 4, 7, 9, 10, 13, 12] that work to repel
mosquitoes. Mosquito repellents based on chemicals
has a remarkable safety profile, but they are toxic
against the skin and nervous system like rashes,
swelling, eye irritation, and worse problems, though
unusual including brain swelling in children,
anaphylactic shock, and low blood pressure [10, 11].
Hence, natural mosquito repellents were preferred
over chemical mosquito repellents.
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Nandini Rani et. al. / Curr Res Microbiol Biotechnol. 2013, 1(3): 98-103
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Nandini Rani et. al. / Curr Res Microbiol Biotechnol. 2013, 1(3): 98-103
Average
wet
weight* (g)
54.48
65.03
74.68
70.63
71.35
76.69
Average
dry weight*
(g)
7.53
12.64
16.14
18.82
22.00
14.88
63.16
9.00
Amount
lost (g)
46.95
52.39
58.54
51.81
49.35
61.81
54.16
Average =
53.57
Dry
weight
(g)
Ash
weight
(g)
Time
taken to
burn
(Minutes)
Residual
(%)
Irritation
Remarks
Citronella leaf
7.43
3.23
12
43.47
Less Irritation
Fully burnt
13.59
2.9
21
21.34
Less Irritation
Fully burnt
9.5
2.4
25.26
Less Irritation
Fully burnt
21.29
5.27
22
24.75
Less Irritation
Fully burnt
13.48
5.22
19
38.72
High Irritation
Fully burnt
21.17
9.85
26
46.53
No Irritation
21.21
10.25
36
48.33
Very less
Irritation
S.
No.
Not burnt
completely
Not burnt
completely
100
Nandini Rani et. al. / Curr Res Microbiol Biotechnol. 2013, 1(3): 98-103
Areas
Laboratory
corners
Department
premises
Supervisor's
room
Cafeteria
Reports given by
people
Smoke caused
irritation
Mosquitoes escaped
Mosquitoes moved
outside the room
Mosquitoes escaped
Remarks
Mosquito repelled
Mosquito repelled
Mosquito repelled
Mosquito repelled
Table 4. Parameters to check mosquito repellency of citronella leaf cakes with different concentrations of citronella oil
S. No.
Name of the
sample
Citronella leaf
Citronella leaf
+ 20% Wood
powder
Citronella leaf
+ 20% Potato
starch
Citronella leaf
+ 20% Corn
starch
Citronella leaf
+ 20% Cow
dung
Citronella leaf
+ 20% Coconut
shell powder
Citronella leaf
+ 20% Neem
powder
Concentration
of citronella
oil (%)
Dry
weight
(g)
Ash
weight
(g)
0
5
10
15
0
5
10
15
0
5
10
15
0
5
10
15
0
5
10
15
0
5
10
15
0
5
10
15
7.1
7.87
7.83
6.98
15.22
13.73
13.96
14.41
18.07
19.39
21.21
20.26
25.7
26.09
24.06
19.79
12.16
16.85
14.1
14.48
9.26
9.39
8.86
8.69
16.2
16.35
15.72
16.54
3.27
3.15
3.05
3.1
3.69
4.28
3.99
5.11
9.9
10.25
10.54
10.78
9.5
9.95
10.01
9.5
5.69
7.02
6.58
6.57
3.85
3.29
3.2
3.19
3.91
5.49
5.16
4.13
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Time
taken
to burn
(mins)
20
15
18
15
20
21
18
19
45
50
40
45
50
45
44
40
25
20
22
21
18
15
20
20
30
25
20
25
Residual
(%)
Irritation
Remarks
46.05
40.02
38.95
44.4
24.24
31.17
28.58
35.46
54.7
52.8
49.6
53.2
36.9
38.13
41.6
48.0
46.7
41.6
46.6
45.37
41.5
35.03
36.1
36.7
24.1
33.5
32.8
24.96
No
No
No
No
No
No
No
No
No
No
No
No
Less
Less
Less
Less
High
High
High
High
Less
Less
Less
Less
Less
Less
Less
Less
101
Nandini Rani et. al. / Curr Res Microbiol Biotechnol. 2013, 1(3): 98-103
CONCLUSION
Currently, the use of synthetic chemicals to control
insects and arthropods raises several concerns related
to environment and human health. An alternative is to
use natural products that possess good efficacy and are
environmentally friendly. Among those chemicals,
essential oils from plants belonging to several species
have been extensively tested to assess their repellent
properties as a valuable natural resource. Citronella
(Cymbopogon winterianus) oil is the essential oil whose
repellent activities have been demonstrated, as well as
the importance of its leaf remains which are generally
thrown as waste are the main focus of this study.
Citronella leaf remains in the form of cakes is the first
and preliminary report. In addition, the use of other
natural products in the mixture, such as binders, could
increase the protection time, potentiating the repellent
effect of some essential oils. On the basis of results
obtained it can be concluded that the citronella leaf
cakes with neem powder as a binder and impregnated
with 10% citronella oil having less residual percentage,
less burning time and irritation is able to repel
mosquitoes effectively is the most suitable mosquito
repellent. This report is the preliminary work done
using Citronella leaf cakes as herbal mosquito
repellents using natural binders. However, further
extensive study by collecting specific number of
mosquitoes in a glass chamber covered with cloth sieve
and exposing them to the smoke generated by the
herbal product with the varying concentrations and
recording of mortality time and comparing with
chemical based formula in the artificial mosquito coils
need to be performed.
102
Nandini Rani et. al. / Curr Res Microbiol Biotechnol. 2013, 1(3): 98-103
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