Wavelength(nm) Absorbance

340
0.11
360
0.21
380
0.48
400
0.76
420
0.77
440
0.53
460
0.29
480
0.18
500
0.20
520
0.31
540
0.46
560
0.60
580
0.66
600
0.60
620
0.45
640
0.30
660
0.17
680
0.10
700
0.04

Absorbance a
0.90
0.80
A
b
s
o
r
b
a
n
c
e

0.70
0.60
0.50
0.40
0.30
0.20
0.10
0.00
300

350

400

Absorbance as a Function of The Wavelength

Corey Lake
0.90
0.80
A
b
s
o
r
b
a
n
c
e

0.70
0.60
0.50
0.40
0.30
0.20
0.10
0.00
300

350

400

450

500

550

Wavelength(nm)

600

650

700

750

th

750

Concentration(M) Absorbance(@420nm) Aborbance(@540nm)

0.0100
0.15
0.10
0.0200
0.29
0.19
0.0300
0.45
0.29
0.0400
0.60
0.38
0.0500
0.75
0.47
unknown #212
0.28
0.19

Aborbance(@580nm)
0.14
0.27
0.41
0.55
0.68
0.27

Absorbance as a Func
Cor
0.80
0.70

f(x) = 14.9636363636x
f(x) = 13.6545454545x

0.60
A
b
s
o
r
b
a
n
c
e

0.50
f(x) = 9.4909090909x
0.40
0.30
0.20
0.10

0.00
0.0050 0.0100 0.0150 0.0200 0.0250 0.0300 0.035
Concentration(M)

bance as a Function of Concentration(M)

Corey Lake

363636x

454545x

Absorbance(@420nm)
Linear (Absorbance(@420nm))
Aborbance(@540nm)

90909x

Linear (Aborbance(@540nm))
Aborbance(@580nm)
Linear (Aborbance(@580nm))

.0200 0.0250 0.0300 0.0350 0.0400 0.0450 0.0500 0.0550

Concentration(M)

Concentration(M) Absobance
1.00E-03
0.0600
2.00E-03
0.100
4.00E-03
0.220
6.00E-03
0.360
1.00E-02
0.580

Absorbance
0.7000
0.6000
A
b
s
o
r
b
a
n
c
e

f(x) = 57.961
0.5000
0.4000
0.3000
0.2000
0.1000
0.0000
0.00E+00

2.00E-03

Absorbance as a Function of Concentration(M)

Corey Lake
0.7000
0.6000
f(x) = 57.9617834395x
0.5000
0.4000
0.3000
0.2000
0.1000
0.0000
0.00E+00

2.00E-03

4.00E-03

6.00E-03
Concentration(M)

8.00E-03

1.00E-02

1.20E-02

(M)

.00E-02

1.20E-02