ASPERGILLUS.

2.2. Isolation of fungi Soil sample for isolation of fungi was collected from the
areas surrounding the same foundry. Petri plates, flasks, test tubes, micro tips
and distilled water were dipped in chromic acid for 24 h. These were then
washed and dried in oven at 95 C. After drying these were sterilized in
autoclave for 20 min at 121 C, 15 psi. Potato dextrose agar (PDA) media was
used for the growth of fungal cultures. Composition of PDA (Himedia) g/l: Potato
extract: 4, Dextrose: 20, Agar: 15, pH: 5.6 0.2. It was sterilized at 121 C, 15
psi. Poured the media in sterilized Petri plates and allowed to solidify for 24 h.
Once the agar was solidified plates were kept in an inverted position for 24 h at
room temperature for sterility test. The soil sample was processed with
isolation procedure using the soil dilution plate method. Petri plates were
incubated at 27 2 C for 4 days. After incubation distinct colonies were
counted and re-cultured for purification. The cultures were maintained on PDA
slants at 4 C. 2.32.2.

Pseudomonas
Isolation and identification of bacterium AM-utilizing microorganisms were
isolated from soil samples with a history of contamination using polyacrylamide
(or) AM monomer in solid minimal medium [composition (g l1 ): K2HPO4, 7 g;
KH2PO4, 2 g; Na3C6H5O7 2H2O, 0.5 g; MgSO4 7H2O, 0.1 g; agar, 18 g; at pH
7.070.2] containing AM as sole carbon and nitrogen source. Soil samples (5 g)
were mixed with 45 ml liquid minimal medium. After incubation for 10 days at
28 1C, a 5 ml portion was transferred to 45 ml fresh medium and incubated
further for 10 days. A

Pseudomonas.