8 pKa -

CM 232 Physical Chemistry Laboratory 36

1.
2.

5709681083 7 2

5709681075 71 ()

25 2559

1. - pH
2. pKa (Bromothymol Blue) -

log
pH

433 nm 616 nm 433 nm 616 nm 433 nm 616 nm

1 11.31
0.028
0.288
2 9.70
0.032
0.291
24.5000 -97.0000 1.3892
3 7.90
0.034
0.201
16.0000 2.3103
1.2041
0.3636
4 6.79
0.104
0.096
0.3421
0.5000 -0.4658 -0.3010
5 5.27
0.129
0.010
0.0099
0.0360 -2.0043 -1.4440
6 3.52
0.128
0.001
0.0200
0.0035 -1.6990 -2.4579
7 2.02
0.130
0.000
433 616 nm
Volumetric flask 2,3,4,56

1 1 616 nm.

2 7 433

 3 1 -7

log

2 435 nm
[

] =

log

0.0320.130
0.0280.032

= 24.5

= log(24.5) = 1.389166

2 616 nm
[

] =

log

0.2910.000
0.2880.291

= -97

= log(-97) =

 433
pH
433 nm
0.14

0.12
0.1
0.08
0.06
0.04
0.02
0
0

10

12

pH

log(In-/HIn) pH
433 nm
2
1.5

y = 0.3105x - 2.0843
R = 0.3856

log(In-/HIn)

1
0.5
0
-0.5

10

12

-1
-1.5
-2
-2.5

pH

pKa y = 0.310x 2.084

y = 0 pKa = 2.084/0.310 = 6.7226

 616

pH
616 nm
0.35
0.3
0.25
0.2
0.15
0.1
0.05
0
0

10

12

pH

log(In-/HIn) pH
616 nm
1

y = 0.1826x - 1.6993
R = 0.1402

0.5

log(In-/HIn)

0
-0.5

10

-1
-1.5
-2
-2.5
-3

pH

pKa y = 0.182x 1.699

y = 0 pKa = 1.699/0.182 = 9.3352



Volumetric flask 1 7
Volumetric flask 1 616 nm
Volumetric flask 1 433 nm
Volumetric flask Volumetric flask 2,3,4,56

log

log

pKa 433 nm log

pH
pH y

= 0.310x 2.084 pKa 6.7226 600 nm

log

pH y = 0.182x 1.699 pKa 9.3352

pH