(Standard Solution)

pHEC

UV
(Calibration Curve
)
5 ( 4 )

()

(/ )

UV

()

1.80
y = 1.984x - 0.3127

y = 2.4276x - 0.0427

1.60

R2 = 0.9902

R = 0.9815

y = 1.3576x - 0.2765

1.40

R = 0.9821

1.20
1.00
0.80
0.60
0.40
0.20
0.00
0.00

0.20

0.40

0.60

0.80

1.00

1.20

(M)
Ca

Mg

(Ca)

(Mg)

(K)

1.40

1.60

 Ca + b A
Y = a + bX r r
1 r

Excel
5 ( 4 )

Excel

[XY
]

 ()

(C)(R)

Initial calibration

Calibration rangeExtrapolation
method

1 5

2 3

4 20% 80%

1Linear modelStraight
line through the originy = axLinear
not through the originy = ax + b
2Nonlinear modelNonlinear
Calibrationy = ax2 + bx + c
Second order or higher order calibration

1Linear regression

Best straight calibration line

Least
squares method

Goodness-of-Fit equation
Correlation
coefficient r 1 0 1
r
0.995 0.990

[Ca2+](M)
0.22
0.35
0.57
0.68
0.75
0.86
0.95

0.41
0.75
1.20
1.42
1.56
1.75
1.98

[Mg2+](M)
0.15
0.35
0.57
0.68
0.86
1.09
1.25

0.25
0.50
0.75
0.90
1.15
1.35
1.55

[Mg2+]=0.75M
=0.95 [Mg2+] M
R2