1.

Explain what the function of the protein test with each test reagent
(CuSO4, HgCl2, HNO3, Pb-acetate)!
Answer:
a) CuSO4 is used to test the presence of heavy metals in protein
characterized by the deposition if positive protein containing heavy
metals.
b) HgCl2 used to test protein-containing phenil hydroxyl group (OH).
c) HNO3 is used to test the benzene ring of salt amino acids making up
the protein, which in this experiment when concentrated nitric acid is
added and produces nitrobenzene derivatives.
d) lead acetate is used to test the amino acids cysteine and methionine,
which in this experiment will yield a black color because S atoms react
with acetic acid forms a precipitate PbS.
2. How does the organic solvent (acetone and ethanol) to the properties of
protein denaturation?
Answer:
Effect of organic solvents on the properties of protein denaturation is a
protein or nucleic acid will lose the secondary and tertiary structure due to
organic solvents can result in denatured protein.
3. List the kinds of ties that causes the polypeptide to be stable in the form
of helix!
Answer:
a) The disulfide bond
Formed between two cysteine residues that are interconnected two parts of
the

polypeptide

chain

b) The hydrogen bond

through

cysteine

residue.

Formed between NH2- group or OH and C = O groups in the peptide

bond

or

-COO-

in

the

group

R.