28/12/2015
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Pulse Oximetry
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Oximetry History
= Becam
1980's
= 1935 Carl Mathes
first oximeter
1940 JR. Squires
self calibrating oximeter
jandard of care in the
Oxygenation
‘TObjective:
‘ensure adequate oxygen concentration in inspired
"988 and blood
{3 Montoring
‘Lined gas oxygen analyzer with alarms (GA)
2Anerilonygen saturation(Sp02)
3 Atrial onygen tension(Po2),
Oximetry History
(Cont'd)
+ 1940's Glen Milliken
aviation ear ~oximeter for use in avitation
research to investigate high altitude
hypoxie problems,
-1964 Robert Shaw(surgeon) built a self
caliberating ear oximeter Which was
marketed by Howlett Packard in 1970 for
Use in physiology and cardiac
cathtorization laboratoriesTerminology Review
*'Sp0, . Non invasive oxygen saturation
+ Sa0, + Arterial (invasive)Oxygen
Saturation (oxygen bound to the
hemoglobin molecules)
= PaQ2. Arterial Partial Pressure,
‘oxygen dissolved in the plasma (only
about 3% of total content) or PO2
= Ca02: Total amount of oxygen in the
blood or the (S202. PaO.
ay
=
Oxygen Saturation
* Percentage of hemoglobin saturated
with oxygen
+ Normal SpO2 is 95-98%
+ Suspect cellular perfusion compromise
if less than 92% SpO2
Insure adequate airway
Provide supplemental oxygen
Monitor carefully for further changes and
intervene appropriately
What are the Normal?
+= 97-100% sat Good gas exchange
+ 90-95% sat: Mild hypoxia
= <90% sat Severe hypoxia
+ Not all patients are the same
= COPD
= Anemia
PULSE OXIMETRY: WHAT
DOES IT DO?
+ MEASURES/DISPLAYS a
-02 SAT OF HbG.
- PULSE RATE.
~ INDICATES PERFUSION
~ PULSATE FLOW
Pros of Pulse Oximeters
PROS
* Non-invasive
= Allows continuous measurement in
real time
= Easy to use28/12/2015
—
eats
CONS
+ Measures Hb saturation rather than the
actual level of Hb, Only measures.
‘oxygenation status.
+ Does not detect carbon dioxide levels in the
blood. C02 determines the ventilation status.
+ Measurements are not always accurate.
Inaceuracy may occur due to nail polish light
Interference, poor peripheral perfusion,
Intravenous dyes, the presence of
Carboxyhemogiobin and hemogiobinopathies,
Cons of Pulse Oximetry
am
{8% Indications for Pulse
Oximetry
+ Uses of Pulse Oximetry generally fall
into two categories
Real Time Indicator of hypoxemia
End point for titration of therapeutic
interventions.
+
# —Pros/Cons of an arterial
blood gas
PROS cons
* Accurate * Invasive
+ The gold standard + Not easy to perform
for measuring fon a patient
respiratory status + Does not reflect
measurements in
realtime status
Technology
he pulse oximeter has Light-emitting
diodes (LEDs) that produce red and
infrared light
+ LEDs and the detector are on opposite
sides of the sensor
= Sensor must be place so light passes
through a capillary bed
Requires physiological pulsatile waves to
measure saturation
Requires a pulse or a pulse wave (Adequate
CPR)
Objectives
* Understand how a pulse oximetry works
(technology)
* Define normal and abnormal pulse oximetry
readings.
* State the indications and limitations when
Using a pulse oximetry in anesthesia ,POCU
and ICU,
=
les
Pulse Oximetry
+ Principle of operation -128/12/2015
ay
ee Pulse Oximetry
Optical plethysmography
detects pulsatile changes in blood volume
aSpectrophotometry
measures pulsatile hemoglobin saturation
Assumptions
all pulsation is arterial
light passes through pulsatile beds
BEER-LAMBERT LAW
ASSUMPTIONS:
= LIGHT PASSES AS A
COHERENT BEAM - DOES
NOT SCATTER.
* SOLUTIONS ARE
HOMOGENEOUS - TISSUE
DENSITY IS CONSTANT.
* OPTICAL PATH LENGTH IS
“PACKETS” GENERATED IN 4
SECOND. (HEIGHT OF THE WAVE).
(Lux)
+ CYCLE - ACTIVITY FROM ONE PEAK
CONSTANT.
aaa = Lambert’ Law
DEFINITIONS a ~—s«Beer - Lambert L
+ WAVE LENGTH - DISTANCE FROM Incident an
‘ONE PEAK TO THE NEXT. iow absorbing sample of |"
(NANOMETERS) 2 :
+ INTENSITY -# OF ENERGY L Concentration ¢ I
=>
THE ABILITY OF A SUBSTANCE TO
ABSORB SPECIFIC PORTIONS OF THE
LIGHT SPECTRUM,
+ WAVE THEORY - LiGHTIS A
CONTINUOUS STREAM OF ENERGY WHICH
VARIES IN AMPLITUDE AT SPECIFIC
FREQUENCIES.
+ PACKET THEORY- LIGHT IS.
‘BUNDLES’ OF ENERGY MOVING AT
TO THE NEXT. (CYCLESISEC =
HERTZ)
+ FREQUENCY - # WAVES PER “+ path length b —>
SECOND. (CYCLESISEC)
=
|e: DEFINITIONS(cont...) f Physics (Beer-Lambert law)
“+ LIGHT EXTINCTION/ABSORPTIO!
SPECIFIC FREQUENCIES,
= Bears laws
‘The concentration of a liquid is exponentially
Felated to tho intensity of ight that will pass
through it. ae m
+ Lambert s Low:
The distance of light t
is exponent
that will pa
led through the liqui
ily related to the Intensity of ligh
through it er
~ Oxygenated hemoglobin sbsorbe a cliferent
wavelength of light than does deoxygenato.
Lbigag "ot" of te ee28/12/2015
Beer-Lambert Law
vans = tine A
Iivans = intensity of tansmited ight
Vine sty of inlgent ght
fraction of ight absorption.
+ = stance lght ansmited tought he
Rous Gam ionain
FP arconraton of sttetemontin
i ‘extinction coeficent of the solute
{@ constant fora given acute spetiog waters
PULSE OXIMETRY: HOW DOES
meetiT WORK? As!
"LR. PHOTOSPECTROMETR’
- HEMOGLOBIN ABSORBS LIGHT.
- THE ABSORBED LIGHT VARIES WITH:
* OXYGEN SATURATION
* TYPE OF HEMOGLOBIN
* LENGTH OF THE OPTICAL PATH.
iam Photospectrometry 45}
‘Photospectrometry is a method of
using light emission or absorption
to
determine the composition of
substances. It generally involves
the
use of light emitters and receptors
coupled with signal analyzers.
fa PULSE OXIMETRY: HOW
x DOES IT WORK? (cont.)
ABSORBENCE CAN BE CALCULATED
* EXTINCTION CO-EFFICIENTS.
* OPTICAL DENSITY EQUATIONS
* BEERS-LAMBERT EQUATION
=a
{M2 WHERE Do We USE
~ PHOTOSPECROMETRY?
= Pulse OXIMETRY
= Capnography
= Capnometry
= Co-OXIMETRY
= Mass Spectrometry
= Serum Glucose (glycolated Hb
2Ac)
Hee Concept of Pulse Oximetry28/12/2015
—>__ Pulse Oximetry
Eeaa ee [BBE cst princine of sPo2
So wavelngte (20 and 960)
‘Staats inctonaleaturaton (ysl satiation
Pulse oximeter
Photodetector
‘Apulse oximeter estimates the amount
of oxygen carried in the bloodstream using,
Infrared technology across the skin :
rs.
Pulse Oximetry principles ae Pulse Oximetry
a + First Principle of operation -
Two main principles: —
* First Principle of operation ~ 1
Infrared absorption by oxygenated and de-
‘oxygenated haemoglobin at 2 different
wavelengths
Hb EXTINCTION
CURVES
‘= Oxygenated blood and deoxygenated
blood absorb different light sources
‘Oxyhemoglobin absorbs more infrared light
Reduced hemoglobin absorbs more red light,
Pulse oximetry reveals arterial saturation my
measuring the difference.
ISOBESTIC POINTS.28/12/2015
How does it work?
* Since there are only two frequencies of
light, only two substances can be
distinguished.
* This comparison is defined as
functional saturation" OR
SPo2=_*%axyhomoglobin
oxyhemesiobin + reduced hemoglobin
—
ms ABSORPTION SPECTRA
1 os
i ss
CALCULATION OF SaO2
+02 Hb FRACTION = —Aes|
O2Hb
O2Hb + RHb + MetHb + HbF + COHb
+ 02 SAT OF AVAILABLE Hb =
02 SAT= _02Hb
(O2Hb + RHB
‘The difference botween 02 sat and 02 Hb
fraction is (MetHb + HbF + COHb + Hbx)
Pulse Oximetry
‘+ Second Principle of operation - 2
‘The success of pulse oximetry depends on
its ability to measure the saturation of the
arterial blood by analysis of infrared
absorption of vascular bed throughout the
whole pulsatile pulse cycle.
jaracteristics of Common H
2 ae
Spectrophotometric
Name Symbol Nermal(%) Peak (nl
Oxy OzHb 97 530
Reduced RHb