MOLECULAR BIOLOGY

Methods:
1. Inoculation of plants with fungal and bacterial pathogens
2. Detection of gene expression
3. Mycotoxin analysis
4. Callose deposition
5. Bacterial proliferation assay
6. Bioimaging with fluorescent and light microscopy
7. Basic methods and tools of Applied Bioinformatics
8. Differential cell organelle proteomics upon infection
9. DNA and RNA isolation
10. PCR, qPCR for detection of gene expression
11. genotyping and phenotyping of transgenic plants
12. transient plant transformation
13. analysis of promoter reporter gene constructs
14. confocal laser scanning microscopy to determine intracellular
localization of proteins tagged with fluorophors
15. isolation and purification of recombinant proteins
16. immuno cytochemistry
17. Immune detection of proteins
18. western blot
19. Heterologous overexpression of proteins
20. protein purification
21. protein-protein and protein-nucleic acid interaction assays
22. analyses of plant secondary metabolites.