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    Degradation of Polyethylene by Trichoderma Harzianum - SEM, FTIR, and NMR Analyses

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    Mamluatul Hikmah
    mikrobiologi lingkungan

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    Degradation of Polyethylene by Trichoderma Harzianum - SEM, FTIR, and NMR Analyses

    Uploaded by

    Mamluatul Hikmah
    10 views1 page
    mikrobiologi lingkungan

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    Degradation of Polyethylene by Trichoderma Harzianum--SEM, FTIR, And NMR Analyses

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    mikrobiologi lingkungan

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    10 views1 page

    Degradation of Polyethylene by Trichoderma Harzianum - SEM, FTIR, and NMR Analyses

    Uploaded by

    Mamluatul Hikmah
    mikrobiologi lingkungan

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
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    24/10/2016

    DegradationofpolyethylenebyTrichodermaharzianumSEM,FTIR,andNMRanalyses.PubMedNCBI

    PubMed

    NCBIwillbetestinghttpsonpublicwebserversfrom1:004:00PMEDT(17:0020:00UTC)onMonday,October24.YoumayexperienceproblemswithNCBI
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    services,especiallyfiledownloads,duringthattime.Pleaseplanaccordingly.Readmore.

    Format:Abstract
    EnvironMonitAssess.2014Oct186(10):657786.doi:10.1007/s1066101438756.Epub2014Jul23.

    DegradationofpolyethylenebyTrichodermaharzianumSEM,FTIR,andNMRanalyses.
    SowmyaHV1,Ramalingappa,KrishnappaM,ThippeswamyB.

    Authorinformation
    Abstract
    TrichodermaharzianumwasisolatedfromlocaldumpsitesofShivamoggaDistrictforuseinthebiodegradationofpolyethylene.Soilsampleof
    thatdumpsitewasusedforisolationofT.harzianum.Degradationwascarriedoutusingautoclaved,UVtreated,andsurfacesterilized
    polyethylene.Degradationwasmonitoredbyobservingweightlossandchangesinphysicalstructurebyscanningelectronmicroscopy,Fourier
    transforminfraredspectroscopy,andnuclearmagneticresonancespectroscopy.T.harzianumwasabletodegradetreatedpolyethylene(40%)
    moreefficientlythanautoclaved(23%)andsurfacesterilizedpolyethylene(13%).Enzymesresponsibleforpolyethylenedegradationwere
    screenedfromT.harzianumandwereidentifiedaslaccaseandmanganeseperoxidase.Theseenzymeswereproducedinlargeamount,and
    theiractivitywascalculatedusingspectrophotometricmethodandcrudeextractionofenzymeswascarriedout.Molecularweightoflaccase
    wasdeterminedas88kDaandthatofmanganeseperoxidasewas55kDa.Thecapacityofcrudeenzymestodegradepolyethylenewasalso
    determined.Byobservingtheseresults,wecanconcludethatthisorganismmayactassolutionfortheproblemcausedbypolyethylenein
    nature.
    PMID:25052326 DOI:10.1007/s1066101438756
    [PubMedindexedforMEDLINE]

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    https://www.ncbi.nlm.nih.gov/pubmed/25052326

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