Lactococcus jimmi Information

Agent Type: Bacterium

Genome Size: 4.64 Mb x (169.28/173.32) = 4.53 Mb
Identifying Characteristics
TEST
Gram Stain

Schaeffer and Fulton

Stain
Catalase Test
Temperature test
Moellers Arginine
Decarboxylase
Carbohydrate
Fermentation

Characteristic
Differentiates
organisms based on
contents of their cell
wall
If the organism
produces spores
Contains enzyme
catalase
Determines the
temperatures the
organism will grow in
Organism posses the
decarboxylase to
degrade arginine
Ability to ferment
specific carbohydrates

Result
Gram Positive
Cocci (in chains)
Negative
Negative
Positive at 37C
Positive
Positive for Adonitol,
Glucose, Glycerol,
Lactose, Malonate,
Mannose, Maltose,
Raffinose, Salicin (gas
production), Sorbitol,
& Trehalose

Other Characteristics
TEST
NaCl 0% & 4% Agar
CAMP Test
Esculin Bile Assay
H2S Test

Characteristic
If organism can
tolerate NaCl
Organism contains
CAMP factor
Organism can
hydrolyze Esculin to
produce iron salts
Organism can reduce
sulfur containing

Result
Positive
Positive
Negative
Negative

Hippurate Test
Indol Production Test
Jordans Tartrate Agar
KCN Broth
LBC Broth

MR Test
Naglers Reaction
Nitrate Substrate
Broth
Gelatin Nutrient Agar
Oxidase
pH range
Semi-Solid Medium
Sheeps Blood Agar
Simmons Citrate Agar
Skim Milk Agar
NaCl 6.5% Media
Starch Agar
Thyoglycolate Broth

compounds into
sulfides
Organism can
hydrolyze hippurate
Contains enzyme
Tryptophanase
Organism can ferment
sodium tartrate
Organism can grow in
presence of cyanide
Organism can grow in
presence of
chloramphenicol
Ferment glucose into
lactic, acetic, or
formic acids
Identify organisms
that liberate
phospholipases
Organism can reduce
Nitrate to Nitrites or
Nitrogen gas
Contains enzyme
Gelatinase
Contains enzyme
Oxidase
If organism can grow
at certain pH levels
Tests motility of
organisms
Isolates fastidious
organisms
Test if organism can
use citrate as its sole
carbon source
Contains enzyme
Casease
Organisms can
survive salt rich
environments
Contains enzymes
amylase &
glucosidase
Detects tolerance to

Negative
Positive
Positive
Positive
Negative

Positive
Positive
Positive
Negative
Positive
6.5-7.5
Negative
Positive
Positive
Positive
Positive
Negative
Facultative Anaerobe

Urease Test
Voges Proskauer Test

oxygen
Contains enzyme
urease
Organism ferments
glucose into
2,3-butanediol

Positive
Positive