Isolation of Jeotgalibacillus malaysiensis sp. nov.

from a sandy beach in Malaysia, with an

emended description of the Jeotgalibacillus genus

Amira Suriaty Yaakop,1 Kok-Gan Chan,2 Robson Ee,2 Ummirul Mukminin Kahar,1
Wei Cheun Kon1 and Kian Mau Goh1
1

Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, 81310 Skudai,

Johor, Malaysia.
2

Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of

Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.

International Journal of Systematic and Evolutionary Microbiology (IJSEM)

Correspondence:

Kian

gohkianmau@utm.my

Mau

Goh,

Tel:

+607-5557556;

Fax:

+607-5531279;

Email:

Supplemental Materials

Fig. S1. A field emission scanning electron micrograph of cells of strain D5T. Bar, 100 nm.

Fig. S2 . A transmission electron micrograph showing the flagella of strain D5T. Bar, 1000 nm.

Fig. S3. Growth of J. malaysiensis in MB medium (pH 11) after 24 h of cultivation.

Fig. S4. Maximum-likelihood tree based on 16S rRNA gene sequences showing the positions of
strain D5T and other related taxa. The sequence of Escherichia coli K12T was used as an outgroup.
Bootstrap values were expressed as percentages of 1000 replications.

Fig. S5. SDS-PAGE whole-cell protein profiles of strain D5T (Lane 1), J. alimentarius DSM
18867T (Lane 2), J. salarius DSM 23492T (Lane 3), J. campisalis DSM 18983T (Lane 4) and J.
soli DSM 23228T (Lane 5). Lane M: BenchMark protein ladder (Novex by Life Technologies,
USA).

Fig. S6. MALDI-TOF spectra profiles of strain D5T, J. alimentarius DSM 18867T, J. salarius
DSM 23492T, J. campisalis DSM 18983T and J. soli DSM 23228T obtained using Bruker
FlexControl 3.3 software (Build 108). The Y-axis shows the relative intensities of the ionic species
detected, and the X-axis shows the ion masses (Da). m/z, mass-to-charge ratio.

Fig. S7. Two-dimensional thin layer chromatogram showing evidence of inter-peptide bridge
formation (peptidoglycan type) in strain D5T, with A1 linked directly to L-lysine as the diamino
acid.

Fig. S8. Two-dimensional thin layer chromatogram of polar lipids from strain D5T. Abbreviations:
DPG, diphosphatidylglycerol; L1, L2, and L3, unknown lipids 1, 2, and 3; PG,
phosphatidylglycerol; PL1 and PL2, unknown phospholipids 1 and 2; and SQDPG,
sulfoquinovosyl diacylglycerol.