Professional Documents
Culture Documents
INTRODUCTION
Standard Methods Agar is a standardized medium for the enumeration of microorganisms from materials of sanitary
importance.
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ATCC
7469
25923
19615
27853
Recovery
Growth
Growth
Growth
Growth
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INTENDED USE
Standard Methods Agar (Plate Count Agar; Tryptone Glucose Yeast Agar) is used for obtaining microbial plate counts from milk
and dairy products, foods, water and other materials of sanitary importance.
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REAGENTS
PRODUCT INFORMATION
g
g
g
g
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IX
PROCEDURE
Material Provided: Standard Methods Agar
Materials Required But Not Provided: Ancillary culture media, reagents, quality control organisms and laboratory equipment as
required.
Test Procedure: Observe aseptic techniques.
Consult appropriate references for information regarding the processing and inoculation of food, water samples and other
materials.1-5
Usually 1-mL samples of appropriate dilutions of the test sample are pipetted into sterile Petri dishes and molten, cooled
Standard Methods Agar is added followed by gently mixing to distribute the sample dilution throughout the agar. Incubate
hardened plates for 48 3 h at 32 1C (dairy products) or 35 0.5C (foods) in an aerobic atmosphere.
User Quality Control: See "Quality Control Procedures."
A single electrode of sufficiently small size to fit into the tubes should be used to determine the pH potentiometrically of
tubed media. The tip of the electrode should be placed below the surface of broth media.
RESULTS
Follow recommended procedures for the counting of colonies and the reporting of results.1-5
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AVAILABILITY
Cat. No.
221030
297943
Description
BBL Standard Methods Agar Deeps (Pour Tubes), 20 mL, Pkg. of 10 size A tubes
BBL Standard Methods Agar Deeps (Pour Tubes), 20 mL, Ctn. of 100 size A tubes
XIII REFERENCES
1. Wehr, H.M. and J.H. Frank (ed.). 2004. Standard methods for the examination of dairy products, 17th ed. American Public Health Association,
Washington, D.C.
2. Downes, F.P. and K. Ito (ed.). 2001. Compendium of methods for the microbiological examination of foods, 4th ed. American Public Health
Association, Washington, D.C.
3. Clesceri, L.S., A.E. Greenberg, and A.D. Eaton (ed.). 1998. Standard methods for the examination of water and wastewater, 20th ed. American
Public Health Association, Washington, D.C.
4. Horwitz, W. (ed.). 2000. Official methods of analysis of the AOAC International, 17th ed. AOAC International, Gaithersburg, Md.
5. U.S. Food and Drug Administration. 2002. Bacteriological analytical manual, (on line). AOAC International, Gaithersburg, Md.
http://www.cfsan.fda.gov/~ebam/bam-toc.html.
6. Marth, E.H. (ed.). 1978. Standard methods for the examination of dairy products, 14th ed. American Public Health Association, Washington, D.C.
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