1.

Principle Benzo (a) Pyrene sampled in ambient air in Whatman glass Fiber using
Respirable dust Sampler and Extracted in Organic Solvent and Analyzed on GC-FID.
2. Apparatus/ Instrument Required:
a)
b)
c)
d)
e)
f)

Respirable Dust Sampler.

What man glass fiber (EPM 2000 Filter paper).
Rotary Evaporator.
Ultrasonicator.
Silica-Gel Column 200mmL,5mm Internal diameter
GC-FID With capillary Column (Length25m, 0.320mm internal diameter
& 0.17 m Film Thickness)
g) Syringes,1-10l
h) Micro-Pipettes,500-1000 l
i) Flask & Beakers, 5,10,25,50 &250ml.
3. Reagents and Chemicals:
a. Standard Benzo (a) Pyrene (PAHs high purity)
b. Toluene (Ultra residue grade)
c. Cyclohexane (Ultra residue grade)
d. Activated Silica Gel (60-100 meshes)
e. Glass Column for Clean up
4. Procedure: Ambient Air Sampling is performs for PM10 using Respirable Dust
sampler for 8 hrs. Using Pre Weighted filter paper Whatman glass fiber EPM-2000
and dust cup.After determined of PM10 and total suspended Particulate Matter by
post weighing the
Entire dust is added to 250 ml Beaker along with filter paper and is extracted with
50ml of Toluene 3 times after 30 minutes ultra sonication each time.
Then the extract is collected in a conical flask (250ml) for filtration.