OLYMPUS INSTRUCTIONS CH30/CH40 BIOLOGICAL MICROSCOPE AXGOO 0ATE SUN Betely Symbola 1, Make sure thatthe voltage selector snitch @ located cn the underside Of the base of the microscope irame is set to contorm with the local ‘mans voltage. Move the switch using an Allen wrench, etc. Fig, 1) When shipped from the factory, the switch is set to 110-120V and “£3-L40V IN 1UWV end ZUOV areas, respectively 2, To.avad potential shock nazerd and tire, always switch the main switch @ to 'O° (OFF) and dizconnect the power cord from tho wall outlet before replacing the bulb. Allow the lamp housing cever @)and the bull 10.cod before touching, 23; Installte misroscape on a leveltabie. The air vents on the underside of ‘the base should never be blocked suchas by placing the microscopeon 2 flexible surface, eg.,a carpet, ete, as this coud resut in overheating and cause a fire 4, Always use the power cord provided by Olympus. If no rower cord is provided, please select the proper power cordby referring tothe secton “PROPER SELECTION OF THE POWER SUPPLY CORD” at the end of ‘is instruction manual. tne proper power cord IS NOt used, UWympUS ‘can no lengar warrant tho olectrical safety ond porformence of the equipment. 5. Connect the power cord corectly ard ensure that the ground terminal ‘of the power supply and that of the wall outlet are properly connected. I the equipment is not grounded, Olympus can no lorger warrant the tlectical safety and pertormance of the equipment, 6. Never insert metal obecis, et. into the air vents of the microscope frame as this wil result in electrcal shock and/or personal injury The folowing symbols are found on the microscope. Study the rreaning of the symbols, and always use the microscope in the safest possible manner. Symbel Explanation Indicates thatthe surface becomes het, and shouldnot be touched with bare hands, Bofore use, carefully read the instruction manual. Imoroper handling could ‘result in injury to the user andor damage to the equipment Indicates that the main switchs ON. A A | ° Indicates that the main swich is OFF© cet nesay 1. A microscope is 4 precision instrument. Handa it with care ‘and avoid subjectng it to sudden or savere imgact. 2. Do rot use the microscope whee itis subjectee to direct sunlight high temperature end humicity, dust or vibrations (For operating environment conditions, refer to andadhore to the conditions specified in Section 6. SPECIFICATIONS on page 16) 3. The tension ofthe coarse focus adusiment knab should only bs aulustee by means oF re tension aqlustment «ng, 4. Heat from this microscope is led away by naturel convection. Consequently, danatuse tin aconetrcted snace and ensure ‘thatthe room is well ventiletes. 5. When moving the microscope, carry t with one hand under the base @and the omer nand holongat herecassed handle fn the rear of the arm @ a shown in th illuetation on the left, Handle the ricrescope with care when moving it + When moving the microscope, never hold the stage or binocular section of the obsarvation tube, ete. Also ‘make sure that eyepiaces, specimen, ate. do not fll of + Sliding the microscope on the surface cf the table may Fig? damage or tear off the rubber feat and/or seratch the Maintenance and Storage 1. Cian all glens sormpenents by wiping gently with gauze. To remove fingerprints or cil smudges, wipe with gauze siahily moistened with a mixture of ether (70%) and alcohol (20%. 4 Do not use a mixture of ether (70%) and alcohol (30%) to clean the lower lens (made of optical plastic) of the eyepiece (NCWHK10X} because such solvents cloud the lans. I'dust adharee to th ‘or wipe it away gently with a dry cloth. Since selvents such as ether end alcoho! are highly flammable, they must be handled careful to keep these chemicals away from open flames or potential sources of electrical sparks — for example, electrical equipment that 's being switched on oF off. Also remember to always use these chemicals ‘only in a wall-ventilated ream. 2. Do nat attempt to use organic solvents to clean the non-optical components of the microscope. To clean thase, use a lint-free, soft cloth ightly moistened with a dlutad neutral detergent, 3. Do not disassemble any part of the microscope as mafunction or damage may eccur. 4 Winen nat using the micioscope, keep it covered withthe provided dust cover Warning Labels Warring lable are affixed at parte where special precaution ie raquted whan handling and using the microscope Always teed the warnings, Waring Label Position | Base Underside | warning bes become soled, peel ot, etc. contact your local Olympus representative to have them replaced GD castion 1 ane microscope is operatea ia manner not spscmed by nis manual, me satety oF me user Maye peri. In 20tition, the equipment may lee be damaged. Always eperate the equipment ax outinedin this instruction marl24 22 54 Assembly Diagram Detailed Assembly Procedure Condenser Focusing Adjustment Knobs . Immersion Unjectives Photomicroaraphy Phase Contrast Attachments.. - 18 . 16 . 10 10 "1 12 13 3CH30/CH40 i Eyepiece } * NOWHKIOX (CHa0) + wiikr0ox (cua Seen We = CH-B45 Tener + CHE-MO4s z 3 2 5 5 ie a Objective *EDAAX, 10X, 40%, 100X oi ‘cHs-c GH2-FCD. arene | Bk-DeD \crs-cop,‘The dagiam below shows how to assemble the various components, The numbers indicate the order of assembly. sembling the microscope, make sure that all parts are free of dust ano dirt, and avoid scratching ‘or touching 130 surfaces. ‘wrench It should be used when units ara replaced or added, Observation ube Condenser ZY) sitaznmon: {optonal for C430) Compensating lens optional for 430), Fiterhalder (fr CH0) fy, 001 natogen bul, 6V, 30 halogen bub (fer chao)CH30/CH40 GI Mounting the Bulb (Replacement) Fg.) 1. Tum the microscope frame on its side and pull he lamp housing knee © on the underside of the base to open the lamp housing cover, 2. Holding the hlogen bulb @ containedin te polyettylene bagto avoid leaving fingerprints on the ou, fully insert the contact prsinto tha bulb ooaket @. When propery seated, pull of vie pulethyete lay, (Appliceble Bulb) (CHZO: 8V, 20WV Halegen bulb: GV20W HAL (Philips 7388) CCHé0: 6V, 30\V Halogen bulb: BV30W HAL (Philips 5761) ‘A Always use the designated bulb. Use of improper bulb may resut in fire, 4.00 not touch the bulb with bere hanks. I fingerprints are ‘accidentally left on the bulb, wipe tho bulb with s sot, lint free oth moistened with alcohol. Using a contaminated bulb will shorten the servie life of the bulb. 3, With the lemp housing knob sil pulled out, clese the lamp housing cover. Then push in the lamp housing krob to lock tha cover + Thwlaip Housing cover cannot be Gosea I'tne kNOD 1s pushed in before you attompt to loss cover. sae 2B Precai ns for Halogen Bulb Replacement ‘A Whenever you replace the bulb during use or right after use, first move themein switch to “O" (OFF, discennactthe power cord from thewall outlet, andallow the bulb and parts around ‘the bulb to coo! befare touching, + Ifthe bulb burns out during an observation and needs to be placed, remove eyapieces, specimen, and other objects like to falloff, before tiking the microscope frame to replace the bulb. Mounting the observation Tube (79.2) 1. Using the provided Allen wrench, loosen the cbservetion tube carping sew ©, 2, Inset the circular dovetal mount atthe bottom of the observation | {ube into the opening on the microscope frame, positonng the ‘bservation tube 10 point the binocular ayapiaces towards the front Clamp the obsenvaticn tube by tightening the camping sorew ©,EB Mounting the Eyepieces (Fig. 3,4) Binocular Tube (CH3-BI45), Monocular Tube (CH3-MO4S) 1. Remove the eyepiese cust cap (©. (Fig. 8) 2Z. Insert neayepiece @ into the eyepiece sleeves fr es tw go. (Fig. 2 '3, Mount the other eyepiece inthe same manner Fi 8 Fig. + ~ [_Using Eyepiece Micrometer Disss (optional) ]- ~~~ Eyepiece mierometer discs can be inserted into the NCWHK1OX feyepieces. Use 10 mn/100 micrometer diss. 1, Unscrew ihe micrometer mounting Frame @ from the eyepece by turring countereloekwieo, 2. Place ® micrometer diss irto the frame, The anara\ine on the 1 micrometer dise should face downward into the micrometer 1 mounting frame @. (Fig. 4) 4 9, Screw the micrometer meunting frame back into the eyepiece =< 1 tube. (Fig. 4) 1% Use 19mm diameter micrometer discs. Fig 4 - a me 7 ©B Mounting the objectives (Fig.5) “Maunt the objectives on the revelving rasepiece in such @ manner that the magnification increases from low to higher powers in a counterclackwise drecion ‘Oli there are empty sockets with no objective mounted, make sure to attech the provided dust plugs © Mounting the Condenser (ig.6) 1. Tun the eoarse acustment knob nse the stage tots highest position 2. Tum the condenser height adlustrrent knob @ te lower the condenser holder'to the positon where the samping screw @) can be turned 3. Insart the condenser @ into the mounting hole @ and clamp by tightening the clzmping seraw@ Positcning the condenser with the iaphragm lever @ at the front faciitates operation. 4, Tum the condenser height adjustment knob @ ta raise the condenser holier 101 highest positonC1 190/01 140 GF Mounting the Blue Fitter ig.7) inuu tw color tare ature of eaylyht io show Ue spouinmnin natal colors Place the 32.5 rm diameter tlue filter ® inthe fer holder @ or the ‘compensating lens. Nounting the Filter Holder orthe Compensating Lens (Fig 8) Insert tne itr holder or the compensating lans into the condenser ‘When mourning the compensating ens, place the lens so thet the ccontering screws @ are at the from Mounting the Field Iris Diaphragm Attachment (CH30 only} \Fig. 9) Holding the folds anhragm attachment @ ove the ight glass exit ‘with the {iter holder notches @ pesitioned es shown in Fig. 8, Insert the atiachment into the light glass exit opaning unt,t seats witha liek ‘ Attomating to insert the attachment with the notches @ facing in other cirections than shown in Fig.9 may breakthe mount- ing lio. El Connecting the Power Cord (Figs. 10,112) A.cabies and cords ere vulnerable when bontor twisted. Never subiect them to excessive force. AiMake sure that the main switch @ is on “O* (OFF) before connecting the power cord. (Fig. 10) By Aisays use the power cord provided by Olympus. If po power cord is provided, please select the proper power cord by referring to the section “PROPER SELECTION OF THE POWER SUPPLY CORD" at the and of this instruction manual.MBLY 5 ase! Fig. 12 1. Connect the power cord plug @ to the AC receptacle @. (Fig. 11) 2. Plug the power cord plug @ into.a wall utr. (Fig. 12) A Connect the power cord correctly and ensure that the ground termiral of the power supply and that of the wall outlet are propeily connected. rant the electrical safety and performance of the equipment. Cord Rest (CH8-cH optiona was When the cerd rest (CH3-CH| is attached on the reer panel of the misroscope frame, the power cord can be wound arourd it for sicrage. Align the hooks @ on the cord rest @ with the air vant slits at @) ‘and inser the hooks nt the sits unti the cord restis pressedagainst the esr pane! of the mistoscope frame. Thon pull the eard rest down to socureit Note: The illustration ofthe cord rest only shows the sections relevant forthe explaration hare, The actual cord rect looks eomewhat diferent + Do not grasp the cord rest when maving the microscope. The cord rast may suddenly come loose an you may drop the microscope causing materiel damage oF personal injury. Deteching—Move the microecops frame tothe oxgo of the table @ Then anniy #cereurtriver ®\er Allan wraneh to tha lowar neice of the cord test Dane push in the [] 2] drections to move the entire cord rest upward tallow it tobe detache.CH30/CH40 Interpupillary distance adjustment scale Diopter adjustment ring Spesianholder Nain iter Lightintensity knob yaxs knob Xaxis knob Filter holder For 45 mm iamaterfters Fine focus ‘adjustment knob Coarse adjustment knob tension adjustment ring \ sFiets irs diaphragm dial "The CH30 is aot equipped with the field iris dlaphiagm function but an opticnal fed iis cfapiragm attachment can be mountedsomo csr snes BM 1 Site the main switch to "|" ON) and adjust the brightness with the light intensity knob. (Page 10), Turn the revolving nosepiece to engage the 10X objective. Make sure ‘that the revolving nosepiece stops with en audible ik Place speciman on the stage. (Page 10) Turn the X-axis knob @ end Yaris kneb @ to move the specimen into the ight path. Page 11) ‘Looking through the right eyepiece with yout right eye, turn the coarse lagustment knob to bing the specimen into focus. After obiaiing approximate focus, use the fine adjustment knob to make fine adiustrren's, Looking through the left eyepiece with your lat eva, tum the aioptar adusimentring to focus the specimen. Page 11)CH30/CH40 Adjust the interpupilay distance of the ayepieces. (Page 11) Center te feds diaphragm ky manipulating the twoccentering screws ‘on the comaensating lens. (CH40.on¥) Page 12) Incase of ©1190, vonuetion Is nut Heuxbery. IF Bie Optona! ‘lela iS diaphragm attachment is maurted, center the attachment in the sarno manner as described above, Engage the obiective to be used for cbservation and adjust th light in- tensity o the desired level, then readjust the focus ‘Adjust the Fela iris daphragm, ‘This only applies tothe CHO il the optional fel irs ciaphragm atiach- montis mounted, Adjust the anerture irs daphragm. (Page 12) 8 34N10300Ud NOLLVAUIEE0 4O AUYHWnScares B 10 © turing on the But (Fig. 15) 1. Switch the main switch @ to "1" (ON, 2. Tutningthe light intensity knob@ clockwisein the direction cf higher ‘numbe's makes the illumination brighter, ad vice versa EB Feels tis Diaphragm (cH40 only) Fg. 10) Using the ‘eld iris diaphragm dial, adjust the diameter ofthe field Its for ebjective power to tre extont that it just Grcumscrbestnetieg. cof wew. When the falc ire diaphragm stopped down to cium scribe the field of view. it will excuda extraneous light and imorove image contrast withn the field of view. * When using 100X objective, the field iris diaphragm image will ‘not be visible within the field of view. Accordingly, stop éown, the diaphragm to its smallest diameter. Q In case of CH30, the field i's diaphragm attachment is mounted on the light ext glass of the microscope base. herrea eh + Releasing the curved finger with great force, 0: suddenly re- ssing your griponthe curved finger knob while releasing tho curved finger, will erack or damage the slide glass. Always release the curved finger with great care. ‘Shacimen Holder for Single Slices 1. Tumn the coarse adjustment knob @ counterclockwise in the direc: tion ofthe arrow to bower the stage. 2. Open the spring loaded curvecifinger@an thespeciman holder and Side the specimen slide inio the specimen holder from the front. 3. Alte placing the slog as fr as nwil_go, genty release the curved fager® ‘Specimen Holder for 2 Specimen Sides 1. Place the first specimen side as described in steps 1 and 2 atove. “Then place the second specimen slide so that contacts the first 2. Gonly release the curved finger @,ing the Specimen Side with One Hand Place te specimen side atthe front ofthe stage, then slide the specimen slide on the stage surface to slowly and gradually open the curved finger in the citacton of tho arrow Insert tha spacimen side ito ihe ssmimen Moder Unt tis fully and properly seated in the soecmen holeer, Cover Glass Use cover glasses of 0.17 mm thickness in combination with objectives marked with the “16(/0.17" engraving for optimum perlormance of thove objectives Specimen Side] Specimen slides with a theknoss between 09 mm end 12 mm are recommenced for CHSOICHAO microscopes. Using hicter specimen sides may prevent correct formation ofthe field iis danhraom tage on tha spectrron cariocs ana tiumination wall not be correct In case of darkfield observation © woving the specimen (Fi. 18) Turing the Y-ex's knob @ moves the specimen in the Yax's drec- tion. Turing the X-axis knob @ moves the specimen in the Xaxis + Never move the specimen by holding the specimen holder of ‘tage directy as this willdamage theknob rotation mechanism. % When further movement of the stage or specimen holder is prevented by the provided limiting mechanism, the tension of the Y-axis and X-oxisknob rotation willincrease. Atthis point, donot rotate the knobs further. Applying excessive force could Sot ve om nonenee EB esting tc icorupiery Distance rig wy While locking through the eyepisces, edjust for binocular vision until the lof ond right fields of view coincige completely Adust so that the two index dots # are horizontal Placing tho interpupillay dietance indexes so that they are parle! With the horizontal ines faciltates the adjustment ‘Also keep the dots paralel with the herizental ines in case of interpupilary distances other than £0, 60, 70, and 75, © Note your intorpupillary distance so-tat it can be quickly duplicated, Diopter Adjustment (Fig. 20) |. Looking through the right eyepiece with your right eye, rotate the: coarse and fine adjustment knobs to bring the specimen into focus, 2. Looking through the left eyepiece with your left ave, turn tho diopter adjustment ing @ to focus the specimen anes sae a 1112 Fig 21 Ssshicom imene 5 Fig. 22 a eRe mose 70-20% A ‘obectve pupt Fig. 28 lise 1. With the 10x abjective engeged and the spesimen arought nto focus, ‘urn the field rie diaohragm dal © counterclockwise to stop down the diaohraam somewrat. (Fia. 21) 2. Turn the condenser height edustment knod @ to bring the field ins iaphragm image ino focus. Fig. 21) 3. While gadually opering the fed itis Caphragmimage, rote the wo centering knobs @ othe compensating ans toadjust so thatthe fila iis diaphregm image is centered in the eyepiece field of view. (Figs. 21, 22) 4. To check centration, open the field iris diaphragm unti its image touches the perimeter ofthe field of view. Ifthe image isnot precisely inscibed in the feld of view, center agein. (Fig. 22) 5. When used for actual observation, open the field iis diaphragm until its image just cieumsctibes the fiela of view. © When the compenssting lens andfield irs daphragm attachment re ‘mounted on the CH30, center the diephragm in the same manner as ‘ascribed above, EB Weture iris Diaphragm (Figs. 29,24) (© Theapertue iis diaphragm determines the numericalapertue ofthe ilurninaticn system. Matching the numerical eperture © of the itumination system with that af ra abjectve provides hattar image resolution and contrast, and aso increases the depth of focus. O Since the contrast of mizroscase specimens is ordinarily low, setting the condenser apertureitis digphvagm to 70-80% of the N.A. of the objective ir use is usually recommended. When necessary, asjust ihe ratio by removing the eyepiece ard lookingiinto the everiace sieeve while adiustina the aperture irs diachrear lever @ untl the imaae shown in Fig. 23s seen,CH30/CH40 © Aciusting the Coarse Adjustment Knob Tension ig 29 1. The coerse adjustment knob tersion is preadjusted for easy use. However, if desired, one can chengo the tension using the tension ‘aajustmens rng (). Applying 8 flet-biaded screwdriver fo any of the g100ve8 Don tho circumference of the ring end turing the tng in the direction of the arrow increases tension, and vice versa, 2 The tension is oa low ithe siage drops by itself or focus quickly loot efter adjustment with te fine adjustment knob @). In this case, turn the ring @ in the drection of the airow to increase tension, Pre-focusing Lever (F.26) The pre-iocusing lover ensures that the objective does not come in ‘contact withthe specimen and simplifies focusing. After focusing on the specimen with the coarse adusiment knab, turn this lever clockwise inthe direction of tne arrow to seta lower init on coarse adusiment mrwament Focusing with the fine adjustment krob is not affected by this pre- ‘ocusing lever. Accordinaly, alter using the coa'se adjustment knob tolowor the stago for changing specimens or applying immersion ell (see Section 6-8), refacusing is easily acsormplished by rotating the ‘coarse adjustment knob to reach the pre-focusing positicn, then making fine adjustments wih tye fine aojustment knob. 1 When not required. Inava the pre-tocsising lever unlocked. Citasimenciene em 1. Fozus on the specimen with a low power objective. 2. Place chop of mmorior oil provided onto the specimen at the area t0 be observed, 9. Tuin the revolving nosepiece to engage the immersion objective, then focus using the fine adjustmert knob > Since any bubbles inthe oil will mpair the image, make sure that tho oilie foo of bubbles, 2. To check for bubbles, remove the avaniace and fully anon the held and aperture ris diaphragms, then look at the ext pupil of ‘the objective inside the observation tube. (The pupil SHOU appear round and bright, », To remove bubbles, rock the nosepieve sligntly to move the oll Immersion objective back and forth a few timer. Ifthe condenser marking shows a numercal aparture (NA) of 10 o: ‘moro, the number appies onty when of is present Between the slide ass end the ton surface of the condenser Whan ail snot present ie NA Is about 0.8 4. After use, remove oil from the objective front lens by wiping with gauze slightly moistened with an ether 7 parts) /aleahol (9 perts) ‘nisture OF with BE-6210, & Using too much alcohol ean diseoive the lone adhesive ACaution on Using the Immersion Oi If th immersion cil comes into contact with your eye or skin, immediately tate the following action. For eye: Rinse with clean water (for more than 15 mi For ekin; Wash with soap and water. If the appearance of the eye or skin changes or ain continues, ase consult your dactor. cc 8 1314 Pp OM comora back I al Pretomieroadeptert | — — Fy Rpgeererine Eo ae Fuiomatic phoionierographic system Peep sa Pai 20 1 AeKpnotosyesice & Mane Au >) Trinccular observation —] tube’ 7] CHS Tras Use the tunoculer cuservation tube CH3-TRAS for photomicrography * Use a heat absorbing fiter(45HA) when using the PM-10, PM-20, and PM-30 photomiccographic systems, GB Selecting the Light Path (CH3-TRAS) (Fg. 20) Othe interpupiiary dstance agjustment procedure of the tinosular ‘obsarvation tube (CH3-TREB) is differant from that cf the binocular observation tube (CH2-BI45). The interpupillary distance dovetail stides © on the CH3-THAS should be moved tomake the adjustment. Mure ure: path selector knob @) 10 Select the required light path, (© For normal observation, te ight pat selector kno should be pushed in For TVand stil photomicrography, pull cut the knob.” Light peti selectoriood | __tntensiy ate ‘Applcaion Pushes in 100% for bineeular Palos out 0 toa Coser of ie eoloves, 80% for ecimens SON jotarme-ograchy, Meretomeraohy | Votaevaton + In case of long time exposure, ambient light in the room wil fente through the observation tube and the evepiaces and may Create ghost imagesor flare. To block this extraneous light, itn the room or cap the eyepiaces or the focusing telescope.GH3U/CH4U, © Mounting the Camera Unit 02) 1. Loosen ihe clamping sciew @ of the NFK photo eyepiece adapter (PM ADA ©. 2, Attach the NFK photo eyepiece adaater [PM-ADF) @ tothe trinocular ‘observation tube (CH3-TR45) @ and tghten the ciamging screw @ 3, Insert the NFK photo eyepiace (3X or 5X} ® into the NFK proto ‘eyeriece adapter IPN-ADFI ®. 44, Mount the camera unit directly on the NFK photo eyepiece adapter (PM-ADTT ©. Using an OM Camera Back “Tose an OM camara back, mount the photo eyepiece adapter PM- ADF@ into which a choice of NFK photo eyepiece is inserted), the photomicroadapter MOM-L@ andthe OM cemera back on the photo {ube in the describes order. ' Use the 3.3% or 5X NFK photo eyepiece. + Focus using tho finder of tho camera back « Photomiaragraphie magrification = Obective magntfication x Photo eyepiece magnification. + To take a good photomicrography with a single lens reflex ‘camera back, a shutter speed at 1/2 second or slower is rec- ‘ommended £0 as to reduce the mircorlockup shock. E@ Focus Adjustment ‘The focus of the eyepieces and the camera fm plane are not the ‘same. Consecuently, always perform camera focusing by using the focusing teescope ofthe camera unit. For detals, eferto theinsiruc- tion manual of the photomicrographic system. ]}© color Temperature Compensation (Fi, 20) Q When using daylight eye film in tre camere + The blue fiter is solely designed for visual observetion (day- light. If this flter is engaged while taking photographs with ‘daylight tlm, the color reproduction wall Be poor. 1. Maunt the ight balancing fiter (A6L.RD-2N) @ inthe ‘iter holder @ of the microscope frame, 2, Turn the light ntensity knob @, To obtain ilumiation close to day- light set the indox ofthe krabat § in cace of the CH39, and 4 in the case of CHAO,BE Nomenciature Light Annu Centering knob cH2-As10 Cantaring Telescope CTS Mounting the Phase Contrast Attachment Mount the phase annulus (C2 1CICII2-NS4O) in the same way e2 the fier holder 4 Replace previously mounted objectives with the phase contrast objectives. Q Botere contation Aor cortration 4. Rotate the CT's uoper lens essembly so that Using the Phase Contrast Attachment (Figs. 31 & 32) 1. turn ine revowwng nosepece to move tne opectve win ine sarre umber 98 te mounted ight nnuilse 2. Set the specimen on the stage and bring it into focus. 3. Remove either one of the eyepieces and insert the centering telescope (CTS imo the eyeniece tube, ve bright ring @ tight Aniki the rondanser| and the dark ring @ inhase annus the objective) core into focus. (Fig. 31) ‘5 Rotate the two ign: amnulus centerrg knobs @ urtl me Hghtannulus| and phase annulus are concentrc and suparimpased.(Figs.31 & 32), 6 Femave the CTS and insert the eyepiece back nt the eyepiece tube topporform shaso contrest observation 7. To increase image contrast, mount the filter cn the fiter holder5 CH30/CH40 me Spetifications ciao Ee ‘cHa0 Optical system LB optical system ffintecorrected systern) Turnination Muminater builtin {6V 20 W halogen bull Philips 7386 {6V 30 W halogen bulb Philips 8761 (Average service time: Approximately 100 hrs, | (Average sence time: Approximately 100 hrs ‘when used as circted) ‘when used es arecteo CHBORFIOO/LF 100: 100/%10—1zZ0V, so7e0 Hz | CHAORFIOOILIOG: 100/110-120V, Sq60 He 103A 100-170V area (0428 100-120V area CHaORF2C0NF200: 220/730-240V, 50/60 He | CHAORFZOOILF200: 220/230-240V, 50/60 Hz (0.188 220-260V area 0210 220-240V area Focusing ‘Stage movement by roller guide (Rack & Pinion) Fullrange strcko: 25 mmm Upper Imi stopper Torque adjustment o7 coarse focus krob Fovolvieg Buld-n quadruple nosepiece Bulson quntupe nosepace receive wth inwar sethinnare ie Seeatsante | Mecca: Tube ircration as Binocular Tube ication 7 cssB.a5 Interpuplay distance adjustment 48-78 mm S08 Sie 186 mmx 134 mm Morenent Specimen hader Dovble sie holier ‘Condenser Type | Abte condenser NA 7.25 (ith ol meron) ‘Aperture ins daphragm| Provided Dirensons ard weight | 2886) S82) 3010) mm 82 i (118) CHI0V3 5 49 (87 CH Operating *# Indoor use sovionment = Atitude: ‘Mex. 2000 m ‘Ambient temperature: §* 10 40°C( 1° to 104°F) * Maximum relative humidity 80% for temperatures up to 31°C (88°F) decreasing linearly through 70% at 34°C (93°F), 60% st 37°C (99°F, to 50% relative humidity at 40°C (166°7) ‘= Main oupply veltage fluctuatione not te exceed 10% of the nominal vaage. + Palltion Degree 2 (n ascordance with IEC 664) * Installation/Overvettage Category lin aecordance with IEC 684) 17OPTICAL CHARACTERISTICS 5 18 Objective designation _ Magnification itn Immersion objenive Cover glass thickness Color band chs0 ‘Optical | Eyepiece Saree Magni:| NA | WD. | REWIOX HI) pe aig Seaton oom ar (past aaaae Objenive re | oe ae pac ax | o1 [2 | sa | ax me | a5 | eterna wx | 025 | e290) 19 | t0K | 200 | 10 ax | oss | os2 | 062 | sox | 200 | 045 |springtonded sox | ton | non | 97 | anne | nan | 03R |Seinoinadnd ieee cao Optical Eyepiece aeF2E| Magni-| NA. | Wo. {WK TOX FN 20) Aa ‘eatin (on) ea roel rag) on Objective : mag ef | Mew DAC x | oi | 2 | sa | ax | us | 50 pereest wx | 025 | 630] 13 | tox 280 | 20 ax | os | oss | osz | sox 3.0%) 08 | sprroiosaos| wox | 120 | ozo | var | wax 00 | ue |spinnenaes| [Stine * The resolving power of immersion objectives is obtained with the aperture iris dlaphragm fully open, Glossary ‘Working distance (WN.O): Thedistance fram the cover glass surface tothe nearest paint a! the abjective, Numericel aperture (NAA! ‘The N.A. value represents a performance number which can be compared to the ralatve aperture (-number) of acameralens. The higher NA. the higher theresolving Resolving power: ‘The abit 10 differentiate two pains, ie.,theminimum distance by which the objects, ‘must be Separated inorder to be revealed as two seperate objects, [As the aperture iis claphraam is stonged down, the focal depth becomes areater. The ‘greater the N.A. of an objective, the shorter the foral depth Field number: ‘A-numier that represents the diameter in mm of the image ofthe field daphragm ‘mats formed by the lens in front off Fiold of view diameter “The actual sie ofthe field of view in millmeters Toxal magnifeaton: guile te objective magnifeation mullified by the evepioue magnificationTROUBLESHOOT CH30/CH40 Under certain conditions, porformance of this unit may be edversely affected by factors other than detects, problems occur, please review the following lst and take remedial action as approrriate, I you canrat sohe the problem after checking the entire Ist, please contact your local Olympus representative fot assistance. 1, Optical System, 2, Fier view s obscure, [Noseaiece is nat eorreety engage. Make sue that he revolving nosepice | Sigur see a ik Ghtees Bropariy ue putea ® beverly ilurinaie. = The condenseris not corectly attached. | Re-insert the condenser all the way |g wwhout tit The field iis dieptragm is not properly | Center the teidiis alephragm, 2 centered. (CH40 only) ‘Tho foldineiophregmie stopped down | Open the Fold io diophrogon [roa te i Dirtidust on the objective. eyepiece. | Clean thoroughly = Condon, andor ight ext gass, ». Dirt or dustis visiblein | DitYust on the light exit glass. tne feld of view. Dienst on the ton lane of the ean denser. CClean thoroughly. = Diryaust on the specimen. Dirtaust on the eyepiece, © Theimage shows | The condensoris lowered too far ‘Adjust the condenser height position. | _ diffraction, ‘re sere 1s deptraums stomped | Open the spate is aah, 2 4. Visibility is poor The objective isnotcorecty engaged in | Make sure thatthe revowng nosepece |g ‘image isnot sharp. the lightpath licks nto place correct. * Contrast poor ‘Details ae indistinct. | Frontiers of the abjectva is diay | Clean the objective = Tnsnersio viis ne bwin used with an | Use immersion ol 13 cl immersion cbjective ‘The immersion oil cortaine bubbles. | Remove bubbles. 13 Recommendedinme'sion oF not used. | Use the provided immersion ol a ‘Specimen is diy. Clean: = Eyopiace, condoncer is dirty Clean = @, Partot theimegeis | The cbjestiveisnot stopped atthe cick- | Make sure that the revolving nosepince | @ Burred, stop. licks inte place comrecty, The specimen is not mounted correctly | Place the specimen correztly on top of | 49,44 conve stage, the stage and secure ft wath the spect | "°° + The magehaca yolow | The blue filter is net engeged Engoge the blue filter. iz tht. 30IND ONUOOHSS TEN a = oO‘TROUSLESHDOTING GUIDE 20 2, CoarsefFine Adjustment 2. Ihe coarse agjustment | ine tersion adjustment ring 1s over ] Loosen na rng ned iehard ton | tightenes e ‘You ate tying to rae the atage withthe | Uniock the pro-foouang lover 3 coarse adjutant ke even theugh the Breocusing lever I ecko tage dis dowa by | The ension adstment ring tao loose. | Tighten the ving 4 during observation «. Coarse agjustment will| The pre‘ocusinglever keeping the stage | Urock the prefecusing lover. 13 natgoallthe way up| cown {. Coarse agjustment will| The condense holder is too low. Fase the condenser holier = stg he way dom The objective mates | The specimen s mounted upside-down. | Mount the specimen correct contact wath the epoct i men before focus is tea Sbisined 3. Observation Tube 2. Fld cf view of cne eve | Theinterpupilay distance is ncorret. | Adust the intorpupilary distance. "1 the other Incorectdopter adjuster ‘Adust the diopter 1 Ditferart eyepieces ave used an the let | Chango one eyepiogs to match the other | — eon Sothat eth sides aro the same, The opteal axes are nat pale, Upon locking into the eyepieces, try ™ Tecking at the overall field. before. ‘Soroantrating cn tha specimen ange You | — fay ago tnd hate to ook ypand mo the distance for» moment before looking | baskinto the miroseo9e. 4.Stage Image bus as veu | The snecien is not correctly pesitioned | Mount the stecimen correct a move ths speciren. | an the stage 5. Objective Change Front lens of high] Spesimen is mounted upside-down. "Mount the specimen corecty 10.1) power ojectve comes | er Into contest with spas | Cover glace io 00 thick Use 80.17 mm tick cover gles, men when it's engages n afar alow power ober te 6. Electrical Syetom 2, The bulb does not ight, | Bulb is net mounted, Mount the designated bul 3 The bulb is bumed out Fepaze the bub, 3 “The power cord plug is net connected. | Connect the power cor. 56 b. The bulb i dirk “he voltage selector switch is set | Set the switch to match the local AC| ineoreetly mains The bub burns out almost | Weng iype of bulb used Use the correct bull type 3 Imnmosatay The voltage selactor switch ie set | Set the swich to match the local AC neoweatly | rains i *CH30/CH40 Optional Unit ‘Application Remarks Brightfioldidarktild phase Centration of phase contrast coniast attachment insert possite, cH2PcO CContration a field ie daphragrn net possible Dry datkield condenser Cantation of darkiield fold of BHDCD view possiole by using the entering knobs of the con senser. Phase contrast attaciment | Centration of phase contrest, HEPC insert possible, Cantraton of fe ins phragm net possble. lard condenser does SW polarized light Simple pobxizna inhtattachment | The CHS-POL is provded with serensPoL ths CHAD. Atigching she com ICostans) pensating lens selow the CH3- | observation. Condenser CH3.COP COP allows centration of the | In case of CH30, centration of Analyzer CHAN Field fis daetregrn the Felis dhoghragin becomes Polarizer U-POT possible if the compensating fons and Nela ing claphragm ‘attachment are acded This device complies with he requirements of both directive 89/236/EEC concerning elecromamnetc ccompatialiy and directive 73/23/EEC conceming low voltage. The CE marking indicates compliance wih the above crectives. —e@ PROPER SELECTION OF THE POWER SUPPLY CORD 22 no power supply cordis provi 1d, please select the proper power supply cord for the equipment by referring to "Sperifications” and “Certiied Cord’ below: CAUTION: In case you usea rom-approved power supply cord for Clympus products, Olympuscan no longer ‘warrant the electrical safety of the equipment Specifications Voltage Rating current rating Temperature Rating Fittings Configuration 125V AC {for 100-120V AC areal or, 260V AC (for 220-240V AC area) {68 minimum 60°C rninimum Grounding type attachment rug cap. Opposite terminates in molded.on IEC configuration appliance coupling Table 1 Certified Cord ‘A power supply Cord should be ceritied by one of the agencies listed in Table 1, ox comprised of cordage ‘marked with an egency marking por Table marked por Table 2. The fitinge are tobemerked withat least one of agences listed n Table 1. Incase youare unable tebuy lecaly in your country the power supply cord whichis epproved by one ofthe agencies mentioned in Table 1, please use replacements aporoved by any other equivalent and authorized agencies in your courtry ee ee ee hasan | Sa Ww | wo ® sta GD w= | wm _ Belgium cesec & NNetherands | KEMA Kens coma | ces wey me ® vennat | vewKo @ | sm ae Frima | @ = mo | © race [ure | swe @ jcomany | voe Qn OYCH30/CH40 Table 2 HAR Flexible Cord APPROYAL ORGANIZATIONS AND CORDASE NARMONIZATION MARRING MEFHOLS Parana Manag Ua eis Gh ane Trimedorgmbossedserme; Blach-Red Yellow’ “Thrend roel Ora Heaton ering iy 36 flavgn ciel sore : Sreanns pa ae czygerecvrcve be ‘conc ewe | w | » | w (VDE) &.V. Prifstelle xvoE> HAR 30 0 10 Bgenrgwoas sewer teare | eco mam | we | w | eae sev chars | 10 | 0 | 50 Flaktratechnic (VK) ‘ 30 10 50 tone Sa ass cham | oo | ow | 80 Electronica Espanola (AEE) ROE Hae m © mw Standardization (ELOT) men a: a = a Iu Porsaes de Quiz Tmt awe Tow |w |v fous sev tam) | Canadian Standaids Association (CSA) SV, SVT, SJ oF SJT, 3X 1BAWG : 23OLYMPUS OLYMPUS OPTICAL CO.,LTD. 243-2 Hatagaya, Shibuya-ku, Tokyo, Japan OLYMPUS OPTICAL CO. (EUROPA) GMBH. Postfach 10.49 08, 20034, Hamburg, Germany OLYMPUS AMERICA INC. 2 Corporate Center Drive, Malvile, NY 11747-9157, U.S.A, SINGAPORE PTE LTD. {291 Rivar Valley Rand #12-D1/04 Vallay Paine Ofica Tower, Singannrn 248973 OLYMPUS OPTICAL CO,(UK) LTD. 2-8 Honduras Strect, London ECTY OTX, United Kingdom. AUSTRALIA PTY. LTD. 104 Ferntree Gully Road, Oakleigh, Victoria, 3166, Australia, Prien in span 2000 02 M4 200-@