DNA RNA

DNA
DNA RNA

1. DNA RNA
10mg () AllPure

DNA and RNA Kit 1ug RNA 1.0%

DNA RNA [1]

2% DNA 0.8%

RNA

DNA Lambda DNA/Hind III

DNA RNA

Marker DNA 23KB

DNA RNA Karlinsey J (1989) [2]

LiCl DNA RNA

Total RNA

Genomic DNA

M |Liver|Spleen|Kidney|Lung|M

M | Liver| Spleen| Kidney | Lung|

Chomczynski (1993) [3] RNA

(Trizol Reagent) DNARNA
DNA
8mM NaOH
DNA
10Kb Magen AllPure
Kits

DNA RNA Nanodrop 2000(Thermo Fisher)

DNA RNA AllPure DNA/RNA Kits

() HiPure

DNA RNA

DNA/RNA Kit RNA DNA OD260/OD280

OD260/230 10mg

DNA RNA 30 DNA

RNA 43ugDNA 14ug

RNA

AllPure DNA/RNA Kit

AllPure DNA/RNA Micro Kit

AllPure DNA/RNA 96 Kit

AllPure Fibrous DNA/RNA Kit

AllPure Plant DNA/RNA Kit

AllPure RNA/Protein Kit

AllPure RNA/Nature Protein Kit

AllPure FFPE DNA/RNA Kit

AllPure DNA/RNA/Protein Kit

g/l
Total RNA Isolation

RNA

DNA

260/230

0.219

2.1

1.98

43.8

0.205

2.11

1.72

41

0.0634

2.16

2.41

12.68

0.0629

2.15

2.47

12.58

0.0698

2.11

1.27

13.96

0.0654

2.09

2.18

13.08

0.0294

2.15

1.85

5.88

2.13

1.74

5.8

0.1493

1.96

0.55

14.93

0.137

1.96

0.44

13.7

0.5159

1.94

1.44

51.59

0.4116

1.94

1.78

41.16

0.1934

1.9

2.3

19.34

0.2502
0.1208

2.13
1.89

2.3
2.76

25.02
12.08

0.1279

2.1

2.44

12.79

0.029
Genomic DNA Isolation

Trizol
Trizol Reagent

260/280

AllPure Kits
20

15

40

30

50

RNA

,A260/280>1.9

,A260/280>1.9

DNA

,A260/280<1.6

,A260/280=1.8

DNA

, <10kb

DNA

20-60kb

2. DNA RNA

3. DNA RNA

10mg ()

100mg (

AllPure Fibrous DNA/RNA Kit 1ug RNA

) AllPure Plant DNA/RNA Kit

1.0% 5% DNA 0.8%

1ug RNA 1.0% 5%

DNA 0.8%

RNA DNA Lambda

RNA DNA

DNA/Hind III Marker DNA 23KB

Genomic DNA

Total RNA
| Heart

M | Heart | Intestine|stomach

| Intestine|stomach

( Genomic DNA )

(Total RNA)

NaNodrop 2000 ()

NaNodrop 2000 ()Nanodrop

RNA DNA Nanodrop 2000

2000 DNA RNA

DNA RNA

Conc.
g/l
Total RNA Isolation
Sample

heart
intestine
stomach

260/280

260/230

Yield g

0.1062

2.13

1.95

10.62

0.0935

2.13

1.72

9.35

0.3947

2.14

2.25

0.3485

2.14

2.28

0.2885

2.14

2.23

28.85

0.2896

2.14

2.26

28.96

0.0291

1.96

1.74

2.91

0.0103

2.28

5.51

2.06

0.1299

1.91

2.07

12.99

intestine

0.1229

1.91

2.12

12.29

stomach

0.0685

1.87

2.22

6.85

0.0625

1.87

0.55

6.25

260/280

260/230

Yield g

Genomic DNA

0.0414

1.88

1.84

6.2

0.0409

1.78

0.39

6.1

39.47

0.0216

2.02

0.15

3.2

34.85

0.0249

2.05

1.56

3.7

0.0266

1.62

0.18

3.9

0.2607

2.2

2.26

20.8

0.9132

2.19

2.09

73.0

0.5272

2.2

1.84

42.1

0.3495

2.22

1.32

27.9

0.1744

2.18

2.13

13.9

Genomic DNA Isolation

heart

g/l

Total RNA

R5111-01

AllPure DNA/RNA
Kit

R5111-02
R5111-03
R5112-01

AllPure DNA/RNA
Micro Kit

R5112-02
R5112-03

1-30mg

385

<10mg
/

R5114-01
AllPure Fibrous
DNA/RNA Kit

R5114-02
R5114-03

458

458
20mg

AllPure Plant
DNA/RNA Kit

R5115-02

385
100mg

R5115-03
AllPure
DNA/RNA/Protein
Kit

R5211-01
R5211-02
R5211-03

R5212-02
R5212-03

1188
5586

1-30mg

R5212-01
AllPure
RNA/Protein Kit

1788
8098

R5115-01

1288
6086

R5116-01

585

R5116-01

1288
6086

R5116-01
AllPure FFPE
DNA/RNA Kit

1188
5586

485
1288
6086
385

1088
5086