Bacteria Count

1. Data collection and analysis

a. The pour plate method

Number of plate
1
2
3
4
5

Number of colony
Uncountable
Uncountable
13
10
9

Bacteria count = Number of colony / (dilution volume of sample applied on plate )

Dilution
1/10
1/100
1/1000
1/10000
1/100000

Count
130,000 bacteria/ml sample
1,000,000 bacteria/ml sample
9,000,000 bacteria/ml sample

Countable (Yes / No)

No
No
Yes
Yes
Yes

b. The spread plate method

Number of plate
1
2
3
4
5

Number of colony
Uncountable
Uncountable
10
8
6

Bacteria count = Number of colony / (dilution volume of sample applied on plate )

Dilution
1/10
1/100
1/1000
1/10000
1/100000

Count
100,000 bacteria/ml sample
800,000 bacteria/ml sample
6,000,000 bacteria/ml sample

Countable (Yes / No)

No
No
Yes
Yes
Yes