Sarah Han

Biomedical Research and Mentoring Program, Muscular Dystrophy Research

Molkentin Lab, CCHMC
Mentor: Justin Boyer, PhD

Muscular Dystrophy

A group of diseases that causes progressive weakness and loss of muscle

mass

Abnormal genes (mutations) interfere with the production of proteins needed

to form healthy muscle

Currently, there is no cure for muscular dystrophy

Maxiprep

Isolate plasmid DNA from bacterial cell cultures

Cell culture
Incubation
Harvesting

DNA Binding
Washing
DNA Elution

What?: What have you learned about the

research process?

Research is labor-intensive

Essential to understand the underlying concepts and

principles behind what you do

Disparity between being in a lab vs. being in a

classroom

So what?: Why does what you have

learned matter?
Research lab environment
Communication, collaboration, and professionalism
Different

facet of medicine

Now what?: Where do we go from here?

Continue to pursue research

RaMP extension Fall 2016

Applications within major

Biomedical Sciences Lab Techniques

course

Read the literature