A.

Tests for measuring hormones and Toxins

1. Involves injection of materials into prepared animals
2. Based in competition of protein binding sites
3. Utilizes radiolabeled hormone
4. Uses enzyme which is attached to an antibody
5. Enzymatic test which measures the rate of NADH produced
6. Uses radiolabeled substance instead of hormone
7. Utilizes fluorescein-labeled drug
8. Confirms methods such as TLC and EMIT
9. Confirms positive test for nonvolatile compounds
10. Allows quantitative measurement of drugs

g. FPIA

a. GC - MS
b. LC - MS
c. IRMA
d. Bioassay
e. HPLC
f. CPB
h. EMIT
i. RIA
j. ELISA

B. Drugs of abuse
1. Used for treating narcolepsy
2. Induces muscle hypertrophy
3. Derived from marijuana and hashish
4. Derived from Coca plant
5. Chemically derived from opium poppy
6. Its major metabolite is phencyclidine HCL
7. Its major metabolite is secobarbital
8. A semisynthetic indolalkylamine
9. A 2,3 disubstituted quinazoline
10. Its major metabolite is morphine

a. Opiates
b. Phencyclidine
c. Amphetamine
d. Sedative hypnotics
e. LSD
f. Cannabinoids
g. Anabolic steroids
h. Cocaine
i. Methaqualone

C. Toxic Agents
1. Otherwise known as Grain alcohol
2. Otherwise known as wood alcohol
3. It is metabolized to hepatic ADH to acetone
4. Common constitutent of hydraulic fluid
5. Has 200x more affinity than CO2
6. odor of bitter almonds
7. Its net effect is tissue hypoxia
8. Converted to formic acid in the liver
9. Leads to CaOx deposition in renal tubules
10. It is mostly converted to acetic acid in the liver

a. Cyanide
b. Ethanol
c. Ethylene glycol
d. Isopropanol
e. Carbon dioxide
f. Methanol

D. Terminologies
1.
2.
3.
4.
5.
6.
7.
8.
9.

Absence of menstruation
Excessive growth of hair
Development of breast tissue in males
Absence of the fallopian tubes
Medullary carcinoma of the thyroid
A.k.a thyroid crisis
Characterized by web neck & short stature
Tumors of the pancreatic islets
Characterized be severe peptic ulceration
10. Chronic excessive production of cortisol

a. Zollinger Ellison Syndrome

b. Turners Syndrome
c. Amenorrhea
d. Cushings disease
e. Hirsutism
f. Gynecomastia
g. Wermers syndrome
h. Mullerian Agenesis
i. Sipples syndrome
j. Thyroid storm