QIAGEN NGS

DNAseq

QIAGEN
NGS

Sample & Assay Technologies

1. (NGS)

NGS

(NGS)
NGS ( 1

) NGS

QIAGEN
DNAseq NGS
NGS
QIAGEN NGS
( 1

A NGS n
B
1. n

www.qiagen.com

QIAGEN NGS 06/2014

2.

NGS

BVII

QIAGEN DNA
NGS DNA
QIAGEN DNA
DNA
( 1) QIAamp
DNeasy Kit QIAcube ( 4)
12

2. DNA EDTA
200 l DNA

1. DNA

MagAttract HMW DNA Kit 200 kb

DNAM:

DNeasy Plant Mini Kit

FFPE

GeneRead DNA FFPE Kit

QIAamp DNA Blood Mini Kit

CSF

QIAamp DNA Mini Kit

DNA

MagAttract HMW DNA Kit

CT

TA
ED

ED
l
2

TA

l
2

Real-time PCR

(HMW) DNA (100200 kb) NGS

DNA ( 2) NGS

MagAttract HMW DNA Kit

DNA

MagAttract HMW DNA Kit DNA

( 3)

An
B MagAttract HMW DNA Kit
3. n

CT WBC

QIAGEN NGS 06/2014

Sample & Assay Technologies

2.

NGS DNA

(FFPE) DNA
CT
GeneRead DNA FFPE Kit FFPE
DNA DNA

4. QIAcube NGS
QIAcube QIAGEN

NGS

QIAGEN QIAamp KitDNeasy Kit GeneRead

FFPE Kit GeneRead Size Selection Kit QIAcube
QIAcube
12 QIAGEN
NGS QIAcube
( )
DNA

1
2
3

5.

REPLI-g Single Cell Kit

DNA
RT2 qPCR Primer Assay

(QIAGEN) real-time PCR

267
CT

DNA

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QIAGEN NGS 06/2014

2.

NGS REPLI-g Single

Cell Kit (11000

) DNA
DNA NGS (
5) REPLI-g Single Cell
DNA ( 6)

(1 )

( 7)

(1000 )

REPLI-g Single Cell Kit

(

(1 )

DNA ) SNPCNV SV REPLI-g

Mini Kit

(1000 )

6. NGS Illumina MiSeq

Bacillus subtilis

(BGI)

2 g DNA REPLI-g Single Cell Kit 105

DNA 300 bp 1 g
A gDNA REPLI-g Single Cell DNA
n
B DNA REPLI-g DNA
n

7. REPLI-g Single Cell Kit DNA

A REPLI-g Single Cell Kit
n

GN S 5
Real-time PCR 3
( G)

( N)

( S)

WGA

REPLI-g Single Cell Kit CT

B REPLI-g
5 DNA n

Single Cell Kit G N

( G)

( N)

( S)

DNA 5
2 X54
G 5 1
99

QIAGEN NGS 06/2014

Sample & Assay Technologies

3.

NGS

DNA

NGS

PCR

GeneRead DNAseq Gene Panel System PCR

( 8)
90%
( 9 2)
85%

85% 10%
( 2)

2.

(%)

AA

KRAS

c.35G>T

38

p.G12V

BRAF

c.462A>C

15

p.K154N

PDGFRA

c.1432T>C

54

p.S478P

TP53

c.817C>T

24

p.R273C

1
2
8. GeneRead DNAseq Gene Panel System
NGS DNA GeneRead DNAseq
Gene Panel GeneRead

ERBB2

c.3590C>G

p.P1197R

KRAS

c.35G>T

30

p.G12V

EGFR

c.1496G>A

p.C499Y

KRAS

c.35G>T

23

p.G12V

PDGFRA

c.1432T>C

59

p.S478P

TP53

c.817C>T

11

p.R273C

Library Quantification System NGS

NGS QIAGEN NGS Data
Analysis Web Portal

www.qiagen.com

QIAGEN NGS 06/2014

3.

GeneRead DNAseq Gene Panel PCR

( 3) Mix-n-Match Custom Panel

9. (GOI) PCR
GeneRead DNAseq System

3. GeneRead DNAseq Gene Panel

Targeted Exon Enrichment Panel

Biology-focused panel:

Cancer Panel (20 GOI)

Biology-focused panel:

High Content Cancer Panel (124 GOI)

Mix-n-Match Panel

124 ( )

Custom Panel

QIAGEN NGS 06/2014

Sample & Assay Technologies

4.

NGS

DNA

GeneRead Library Prep Kit

DNA

10. GeneRead Size Selection Kit

50% ( 11)

Life Technologies Ion Torrent Illumina HiSeq

Illumina

MiSeq 50 ng

FU

( 12)
GeneRead Size Selection Kit DNA
QIAcube
( 10)

I
250

HiFi
( )

HiFi
( )

5
4
= 150
= 45

11.

Illumina
DNA GeneRead
Library Prep (I) Kit

125

DNA
Supplier I

1
3
= 100
= 5

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QIAGEN NGS 06/2014

4.

qPCR

(%)

(Ion Torrent ) (Illumina )

GeneRead DNAseq Library Quant Array
PCR
( 13)

()

(%)

()

QIAGEN
L

13. GeneRead DNAseq Library Quant Array DNA ( 5

10 )

QIAGEN GeneRead DNAseq Library Quant Array

NGS ( 14)
NGS
QC

A
12. DNA n

GeneRead Library Prep (I) Kit DNA (50 ng)

Illumina DNA Illumina MiSeq
49
B DNA Ion Torrent DNA
n

GeneRead Library Prep L Kit DNA

DNA

DNA
DNAPCR
()

14. QIAGEN
GeneRead DNAseq Gene Panel

DNA
DNAPCR
()

QIAGEN GeneRead
DNAseq Library Quant Array DNA

QIAGEN NGS 06/2014

Sample & Assay Technologies

5. NGS / 6.

NGS

NGS
NGS PCR PCR
QIAGEN NGS
Illumina MiSeq HiSeq Life Technologies Ion Torrent

GeneRead DNAseq Targeted Exon Enrichment Panel NGS DNA

( 15)
NGS
1. FASTQ SFF
2. 30
/

3.

15. GeneRead

GeneRead
(Ion
Torrent PGM Illumina)
(
) (
) NGS

10

www.qiagen.com

QIAGEN NGS 06/2014

7.

MagAttract HMW DNA Kit (48)

MagAttract Suspension G, buffers, Proteinase K, RNase A

67563

DNeasy Plant Mini Kit (50)*

Spin columns, RNase A, buffers, collection tubes (2 ml)

69104

QIAamp DNA Blood Mini Kit (50)*

Spin columns, reagents, buffers, collection tubes (2 ml)

51104

GeneRead DNA FFPE Kit (50)

Spin columns, reagents, buffers, collection tubes (2 ml)

180134

REPLI-g Single Cell Kit (24)*

Polymerase, buffers, and reagents for 24 WGA reactions

150343

GeneRead DNAseq Gene Panels

Wet-bench verified primer sets for targeted exon enrichment

180941

GeneRead DNAseq Gene Panels

High-Content

Wet-bench verified primer sets for targeted exon enrichment

180942

GeneRead Custom DNAseq Gene Panels

Primer sets for customized targeted exon enrichment

180946

GeneRead Mix-n-Match DNAseq

Gene Panels

Primer sets for customized targeted exon enrichment,

selected from QIAGENs laboratory-verified primer sets

180944

GeneRead DNA Library I Core Kit (12)

Buffers and reagents for end-repair, A-Addition, and

ligation, for use with Illumina instruments

180432

GeneRead DNA I Amp Kit (100)

Buffers and reagents for library amplification, for use with

Illumina instruments

180455

GeneRead Adapter I Set 12-plex (72)

12 barcoded adapters for ligation to DNA library, for use

with Illumina instruments

180984

GeneRead DNA Library L Core Kit (12)

Buffers and reagents for end-repair, ligation, and nickrepair, for use with Life Technologies instruments

180462

GeneRead DNA L Amp Kit (100)

Buffers and reagents for library amplification, for use with

Life Technologies instruments

180485

GeneRead Adapter L Set 12-plex (72)

12 barcoded adapters for ligation to DNA library, for use

with Life Technologies instruments

180994

GeneRead DNAseq Library Quant Array

PCR arrays and components for library quantification

180601

GeneRead Size Selection Kit (50)

Spin columns and buffers

180514

QIAcube

Robotic workstation for automated purification

Varies

* Larger kit sizes available.

QIAGEN QIAGEN
www.qiagen.com QIAGEN

Visit www.qiagen.com/goto/NGS and stay up-to-date on innovative NGS solutions!

Trademarks: QIAGEN, QIAGEN GeneRead , QIAamp, QIAcube, DNeasy, GeneRead , MagAttract, REPLI-g (QIAGEN Group); Ion Torrent (Life Technologies Corporation); HiSeq, MiSeq (Illumina, Inc.).
8075950 06/2014 2014 QIAGEN, all rights reserved.

QIAGEN NGS 06/2014

Sample & Assay Technologies

11


021-3865 3865
800-988-0325

400-880-0325

TechService-CN@qiagen.com
www.qiagen.com

8075950 06/2014

Sample & Assay Technologies