sex ual of Baie Sees Fates Pet 1 Ain 2008p. 38 Studies on Produetion of ibiotic from Teunami sol isolates of ‘Streptomyces sp at Velanganni, Nagapattinam Distriet (Chanthurw A", N:Yogananth, RBhakyaraj Arun Susi and S.Palaivel PG and Resarch Depart of Mirobilo col of Ars and Scene. Puch 622.42, "anil Nod ni. "actu Daprnnt of Boy, LH. aj slg or Me, Puc, ai Nac, din *Carapondng thr: beyoganigmal con sxprmigmalle Receved 1 March 2008; viewed bss iy 208; ec 3 iy 2008 “asus pneaedby Ser disbursed wit volcanic ris olan ies | Soil samples were cllsed from tram’ affected res and their physicochemical characes aay 0 | AsestneGtrulon of bce ne fn was observe Ta ol as ou cs suchas | Ph i A tol ofS genera baci viz, checks eal, Sepiiocxcas vines, Phndeson | ergo Baca cere an Bi sbi ee gh, Cand liam were ote. wien tothe, Actinomyces membre wet alo enumerated, The elrotirextacts of Sree ute | ‘ies (0pm wer sree for amici acy Twa sepia lies wee wed, was Ose ed | that Codd bcos Psendononswrainrawerehighy este (0.0t0$80 mn and25.0010 6081 | an specie owed Ba cro (2642 and Stephens anes (7 9 392 Inhibit act ofthe skis apse tbh to Baca sbi (11102862 0) fa Esherih col. 09 30.2 mu) hea canpasd Keywords: Tuna si, pyseo-cemial charac, Sapanyees sp. inci aiviy Introduction ‘The Tunaml of December 26,2004 has cause economic snd eologcal ester in 13 counties. ius pete deo musive undercut, messed 9 inthe Richer sale of Sustain Indonesia. Beyond the heavy toll of human lives, the tndian Osean carthquake has caused an enormous environmental impact on the region It hs ben reported tht severe damage has been iflicted on ecosystems such ss ‘mangroves, coral reels, forests, animal ai plant ‘avers’ and groundwater In ado, te spread of solidand gut wast and indus chemicalsand wer palltion caused by destruction of sewage collseiorsand ‘retment plats threaten he vironment ven Tut, » inumokl ways. Acoaingtopcsint thenain dans isbeing casey pinning of esate saplesand thsi sea wate auton and the depen ah layer over able ld (Stat Writer, 2905) I is wel ‘that magia damages man he case by sa water evotaminuod by excreta (Kom et ul 187). tthe marine ey soos seh sala, marshy plas, plat Hite dconosie dead iba aia ports are socal ak theshore and btm sediments whic provide nitrons for avarayofnicronpnisnsespially er sapoyhytic Figl and eters Sypris spss ane te ng tibiae producing meres ia the medial worksoSis o Proton of abit fom Tuam soa of Srsptomycr pat Velangamn, Nagata Dist fa. The numberof antimisrobial compounds ported from the species ofthis genus per year has increased almost exponentially for about two “decades. Recent reports show that his group of microorganism st ‘eons animparat source abit (Wave, 2001) Streptomyces (order Actinomycetales, family ‘Streptomyestacene) are gram positive filumetony bacteria hat are ubiquitous in sil and produse more than 70% of the known antibiotics (Tanaka and Omura, 1990), Antibiotics produced fom Sieprampcor ean ‘inhibi abroad range of soil borne robs inlading 7am postive bacteria und fungi (Ehabyad ea, 1993 Lin etl, 1996 Xiao eral, 200) Actinomycetes having sntimierobial activity in Egyptian sll anraced the attention of some investigators (Abu-Zeid and Shas, 1971; ELGamme! a, 1993. In the present study, the isolation and huracterizaton of Srepiompoes sp tom tami si a5 well a the inhibitory effets ofthese isttes were ‘ese aginst vations bacteria ung and ati Materials and Methods Scolsampes were collected from tsunami aected sta ses at elangaoni, Negaptinam Distt, Tamil [Nau nda. The simples were taken from 6 to 10 em depth afer removing apreximatsly 3m of the sil surface, Thre stes wee selected sie 1 wa situated ear thetidl endo sa sie M was 28cm aay fromthe Site and site which was 25 cm away fem the sie tt both towards the shore, The samples were placed in polthene bags, closed tightly and storedina efigsator For the sty of physico-chemical characte, sel samples of I each was analyzed for lene Pll, electric conductivity, total nitrogen, available phoephors,avalable potassium and mieronutrents (Crosser, 1970). Thesis an fang srsins wens ‘sole using tin plating tecigue Die angles vere taken up fo 10°19 1 lations and pled on ‘Natiewtagar for baceria an Pt denon aga plates Foe ings, For isolation f Srepone py the il was po uated with CaCO, (10:1 and ines PC forays. lwasaben suspended in stings slain (4 strength). Test tues ening 10 dilaton samples ‘wae placed ina waterbath 1° for Ihr th the spores woul separate fem votive el led ‘samples were insulated into fementation medi (eh Yeomprisng:picose: 40. provost Sg pepe 92. CCO,:6 8. MeS0, 0.1m. eobel chlorite: 0005 mg stile water 100 land inet fo 24 fs at 259 (Alexandr, 2001), Th active Semmes isolates were subjected to morphological and biocemica tests (Hot 1994). Chloroform extats of Srepiamees sp eure filtrate (0 ppm) were concentated and tes for thee antimicrobial activity against Tsunami sil lates of Bichercha colt Suphylococs ares, Pemba ‘eraginoso, Belts cores, Bais subi and Candid lbieons. Fier paper discs (6mm in diameter)Ree ui hg See ‘nus et Au Kp. 38 wereimpreguted withered both, i a placed on to Mille Mlzon agar plats separately seeded with the above bacteria and nga cult. The plates were incubated a3 and examined for ef hibition, Results and Discussion ‘This study was cared outtoasiess the dstbution of bata and fngus in Tsuna sci at Velangann, ‘Nagapuinam Dist nd also to asses the poacion of enbotc frm Sreptomyces isolated fom Tsai aifeta si, The pysioochencal characteristics ofthe ‘soll ample the pH were nearly neutral alkaline (pH 7880) and total suspended soliés were maximum in sit (823724) and mien insite Ut (80957.0). 4 high degree of salinity was observed inal sites, The emcenrtiono iron was bservediobeexerel high ‘The sol fom the sea carying ox chemicals ie Fe, Pb, Hg in smal amounts seriously redces soi ferity snd agricola ouput (Harison etal, 984)-Theresuls vee tabulated in Table 1. The total umber of acca ‘soot nce five genera represented by Escherichia call EC), Saplyfocoecus oures (S.A), Psenononas caeruginosa(PA), Boils cores (8.0), Bac subilis (5) and ove fang, Candid ois (C.A) fom the vious susie. A total of 8 diferent Sroprompecs isolates wore recovered fom tre sol samples. Outof the 8 slates of Srepiomyces, two wee foun 40 be dominant and and grown well on AGS medium, Morphologi boehemia! properties ofthe promising stain were Fran tobe sini ot dsr by Witla it (oss (1971. 5d cule ses reveaed he press of sabstate mycelia a wel as ata myestian one ‘wth ite istry py pein ‘he grey clon oats pa Hight ow pgm ‘wih diised iat Ye medium and hah he sets ud spirals of spore eins on he arial mycelia. The to isolates showed similar yrowrth ptr, ot slate 1 was comptvsly slug ad hoe hase was wtiged til 48 hrsand the stationary phase ostinued vp tol ‘The aninirobial sev’ of Sreptomyves shown in this study was eptvted by Edwards, (1980) and gor (1985) Dats on antinicrobial activi ofthe chloroform extract of th wo tes were preset in “Table 2and Figure |The extstof oneday ideale id not show any aninirbil activity ence Ecol Auth one from 4h cults inhibited Cabins, coll and Paeruginosa. The increasing wend was maintained upto iveday. The growth ofall sites organisms ws inhibited toa yeat extent bythe erat tsind fom $dayold culture. The subsequent decline in antimicrobial activity was ommensurte withthe Aerease in the growth othe to slates. bay sd Pogrgnasa were highly seesine wo the exe of ‘Sroponyeos isolates 1 (4.0 0 58.0 mm and 250010 6081 mm respectively) (Fg) allowed by Bacilon ce (28 9 42a) ad glacier (2700 1039.62 mn. astviy ofthe slate appeared tobe high or Herc cali 0.0040 30420) hon‘ules on Praducion of nite ro Tana sil le of Srpompee sp at Vega, Nazapainan Dist compared to Bact suits (11 w 2.62. ote Ih ‘ured out be lea activ sit inhibited the growth of ‘aly Calica and Pacinos ad forthe cher tot Sraptaycersp romin care stationary pb ainsi ws eer mid or nefecive. The ty tag Naber revels hat Sreplomce 9 3 proisng san for NN sibs rotiction ® 48h The ‘ble Pino sbmic ees fea sis Tara | Nia mae | a * sna S37] par [ be | 18S [ore oamiy) w [ oP, [ay | Fe | Po | te | Me T | sae | 7a | wos | 023si0 | 326s | 790] so | 213 | v2 | tos 120 CA bel 2| sien| ao | ss | soso | smsro | ino || a0 | 210 | von | 12s | ase] 9 + |smein| 18 | 803 | gaara | os27m2 | 1ss2 | 43 | a0 | 216 | 601 | 132 | os 2| is he ase “il Antinori | tacts | rai [Goma | se ema] ant | sen] mm | St | ut cae cite | ttt | Cam) | tu ort) uy | amy | M 000 wo 70 25.00 vsoo | i190 | somM aw | ow | no | 20 cam | 300 | 200 | teow n | me | me | see sso | anu | ou | oom | so | 306 | we con | 2m | 0 | loomCA SA SA PA BC BS‘Sues on Potion of abi Fm Tau a oles of Srptoncer op at Vlg, Noggin Diet AA and Shebeta YM. (1971). Z. Allg, Miorbil 1475-483. Alexander $ K end tte D. (2001, File paper dis: ‘method, In: Microbiology photographie als Fre labratary, Addison Wesley Longman, Ine New yk, lo2t08. 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