Storage influence of dry leaves in the essential oil of patchouli (Pogostemon

cablin Benth.)
The essential oil was extracted by hydrodistillation method Clevenger type
apparatus, using 50 g dried leaves, expressing the results in% (based on 100 ml
g-1 dry weight).

The qualitative analysis of the essential oil composition was carried out by gas
chromatograph coupled gas to mass spectroscopy GC-MS mass (Shimadzu QP
5050A Model) equipped with an AOC-20i autoinjector (Shimadzu) and silica
capillary column molten J & W Scientific DB -5MS 30 mx 0.25 mm id, 0.25 mM of
film, using He as carrier gas with a flow of 1.2 ml min-1, while maintaining the
temperature was set 50 C for 2 min, followed by a increase of 4 C min-1 up to
200 C, then at 15 C until reaching 300 C keeping this temperature steady for
15 min; injector temperature 250 C and 280 C temperature detector; 40 mg of
the oil was diluted in 1.5 mL of ethyl acetate and injected into a volume of 0.5 uL
of the solution; volume injected partition ratio of 1: 100 and the pressure of 64.20
kPa column. The conditions of MS were ion capture detector operating by electron
impact and impact energy of 70 eV; Scan speed 1,000; scan interval of 0.50 s-1
fragments and fragments detected in the range of 40 to 500 Da.

Quantitative analysis of essential oil components was performed in a gas

chromatograph equipped with flame ionization detector (FID) using a Shimadzu
GC-17A equipment, under the following operational conditions: Capillary column
ZB-5MS fused-silica with 30 mx 0.25 mm id x 0.25 uM film, using the same
conditions as GC-MS. The quantification of the components was performed by the
area normalization (%). The concentrations of the compounds were calculated by
the area and placed in GC elution order.

The oil components were identified by comparing their mass spectra with spectra
existing in the literature, 20 with the database spectra (NIST21 and NIST107) of
the equipment, and also by comparing retention indices with those of literature.
The Kovats retention indices (KI) were determined using a homologous series of
n-alkanes (C 8 -C 18) injected in the same chromatographic conditions of the
samples, using the Van den Dool and Equation Kratz.21

Mean variables were subjected to analysis of variance and compared by SkottKnott test at 5% probability