Sarawak Biodiversity Centre

Industrial Training Weekly Progress Report

Trainee Name:

University / Institution:

JONATHAN CHAN CHIA JUI

Lab Attachment:

UNIVERSITY MALAYSIA SARAWAK

Training Date:

MOLECULAR BIOLOGY LAB

6/7/2015

No.

Task/Activity/Project

WEEK 10: 7.9.2015 11.9.2015

Progress/Status

Remarks

PCR product purification

Assisted

Faint bands.

pET28a plasmid isolation

Accomplished

DNA pellets were vacuum-dried.

Preparation of SDS-staining solution

Accomplished

Overnight cultures of BL21, DH10b, AsPKS C8, and AsFLS2 colonies

PCR4TOPO-AsPKS isolation

Accomplished

Preparation of BL21 competent cells

Accomplished

500 mL culture. Two centrifuge bottles contaminated with

DH10b cells.

RE digestion of pET28a and pCR4TOPO-AsPKS plasmids.

Accomplished

Purified cut plasmids and AsPKS concentration were

determined

Verified by Supervisor:
Supervisor Name:
Signature:
Date:

SBC-ADM-GEN-SPE-LIX001-F06 Rev 0

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