Biol207(

Lecture(23(
DNA(sequencing(

The Nobel Prize in Chemistry 1980

Frederick Sanger

Walter Gilbert

A+C+G+T
4 different fluorescent
labels
in one reaction

capillary
electorphoresis

Laser

Classic Sanger method

dye

detector

Advantages
1.Produce large volume of data in a
timely and inexpensive manner
2.Quantitative
3. no prior knowledge of a gene
required
4 comparative evolutionary studies

 Roche 454- pyroseqencing

Illumina Solexa- cluster reversible
terminators
Helicos BioSciences -single molecule
reversible terminators
Life Science/ABI-sequencing by ligation
Pacific Biosciences-real time
sequencing
Ion torrent

What is pyrosequencing?
Pyrophosphate
(PPi)

Pyrosequencing(
h:ps://www.youtube.com/watch?v=bNKEhOGvcaI(
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Helico(single(molecule(
h:ps://www.youtube.com/watch?v=_MMK3MGfPvY(
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