UNIVERSIDAD INTERAMERICANA

RECINTO BAYAMON
DEPARTAMENTO DE CIENCIAS NATURALES Y MATEMATICAS
BIOT 3570
PROF. EMELY MORALES COLON, MS

TECNOLOGIA DE DNA RECOMBIANATE- ITINERARIO DE LABORATORIOS

Semana Das

Laboratorio (mircoles)

Mircoles: 26 de enero

Laboratory introduction

Mircoles: 1 de febrero

Micropipettes and solution preparation problems.

(THIS EXERCISE WILL BE USED IF THE COMPONENTS OF THE
SOUTHERN BLOT ARE NOT AVAILABLE YET)
Southern blot (electrophoresis and transfer) ( Edvotek #207- Unit I
and II)

Mircoles:8 de febrero

Activity 1: DNA Fingerprinting by PCR Amplification ( Edvotek #130) .

(THIS EXERCISE WILL BE USED IF THE COMPONENTS OF THE
SOUTHERN BLOT ARE NOT AVAILABLE YET)
Southern blot (staining and interpretation) ( Edvotek #207- Unit II).
Activity #2: Plant seeds for genetics lab.( Edvotek #336 module I)

4
5

Mircoles:15 de

Activity #1: Plant Genetics (DNA isolation). ( Edvotek #336 module II)

febrero

Activity #2: Seed worm plates.( Edvotek #858 module I)

Mircoles: 22 de

Activity #1: Plant Genetics (PCR). (Edvotek #336 module III)

febrero

Activity #2: Grow worms. ( Edvotek #858 module II until step 5)

Mircoles:1 de marzo

Activity #1: Plant Genetics (electrophoresis). ( edvotek # 336 module

IV)
Activity #2: Observe worms. ( Edvotek #858 module III and step 6 of
module II)

Mircoles: 8 de marzo

Activity #1: DNA Damage and repair ( edvotek #957 module I)

Activity #2: DNA isolation from worms. ( Edvotek #858 module IV)

Mircoles:15 de marzo

Activity #1 : DNA Damage and repair ( Edvoteck #957 module II)

Activity #2: Amplification of sequence from worm ( Edvotek #858
module V)

Mircoles:22 de marzo

NO CLASES

10

Mircoles:29 de marzo

Activity #1: RT-PCR (RT and PCR). ( edvotek #335 module I)

Activity #2: Worm PCR electrophoresis. ( Edvotek #858 module VI)

11

Mircoles:5 de abril

RT-PCR (Electrophoresis and Analysis) (edvotek #335 module II)

12

Mircoles:12 de abril

Semana Santa NO CLASES

13

Mircoles: 19 de abril

DNA/RNA Microarrays.(Edvotek #235 )

14

Mircoles:26 de abril

Bioremediation I (edvotek #956 module I)

15

Mircoles:3 de mayo

Bioremediation II (edvotek #956 module II)

16

Mircoles:11 de mayo

Review for final test