CAMELIA PAPUC ANETA POP MIHAI SERBAN BIOCHIMIE MEDICALADBIOCHIMIE ANALITICK CANTITATIVA Obiesvele i scopul deteminilorBochimie ct» iota obtneea de date anaitce informa recor tfetoe le Weta ‘ute sle ‘unos componente. bochinice ula prdele osions alate, ca telecire a unor proce ishole nongale me pcan ie reflect rapt crave, mute dine sbordare cnet ‘ests, conde pu de propre mdi oom aca probeor ce ume a alate, eaeporel, peers tl eal propre parecer nin le epee ee ‘croarea uncadnreveria onduen fears teu Be eee one sttple menionate tebe parce cre, penta cx tela) fal oh sonstdent. Boch clini eae wld doen abe is Insert inte hue sf eacue aladdin chose ctl tiie vo mei stl cmap yr i, ‘a cate ine dn cre movin infemaigSneles cueing in seopel ali seni clit, potle bike pat stnalizate pe doud cti: eee ea coy chemist) cae ot efit iar ed tin O van sce tai nde apo a Fide (eesingeeso). Acta saplfel meal oes fein sale ealcncice, ead Yolen mel Se pots Slog wi fet poutine ajc immediate tor foe sf pram pf rpc confit component cine eat (26 epi eal pie ae nl dee 3 Se ingeoate x reac speriet inte wrt care en ts sn ior de taal pat. Po ncn prt lle enti (Gt en) eci flan ba ee ew Rech torrent anmte eee sialic he ee resctvlr,detemindnd spr sau vewren une ‘le nesta lor deve ee proporonal In anit lite ~ cu cone ampaneriss) vesigat’Henfowea sau eraharea compre ‘occ se seize pia compare cao aa suo! eft, seal Isc Ta prospec supe abal feet tern Tens "ape outst eeavanajl noma lor “emieiie ot flote dp expres prion de gr, an ae Soin Valo erent Sipe Figura Repreznaes schematic wu a int eel pide “enaliza ume” (wet christy) ~lnlode arab deter efecto prin metoele species eins (oshlnl) anion, il soli de reat. Asst Sp de soit, ape date “nalica We", ete mull mal pec, reruntle peste ind atresia xl ber sno ce nor parame bende pa ese ape vat pit Ire nce. ae one ope acu spt, Dae oer Suir auomatzatl set sinsoraioe per ‘elie urapsinte a una seta de arate pt tetra. [EXPLORAREA BIOCHIMICA ASANGELUT Consider generate ‘Stipe component ea mi nie is ier, presi net iid ont din element we loa “lemetlflrte sa een le apes age haat ont 5 sect itlle_ te sare (ronontsea), ~ onto leit ang) Plosma snp cmponera ogel, eco 2 ‘gnacie (plinermils),+ pa 0% ‘rganice (6) — lucie, lps, prod, entine, vaming, ‘ornoni— pi compovente anraic (19) ~ nif sao snorgani Stage, pin ote components sl, cele pi plasma inte bs cre tr oe 2 salt uma proctor staolce la cyan ea vol speciale proba feels Loe (hi ea tie a ‘enim menjinern Ancor singel. Modif als volamist decraiad ruta enact nari Congularea singel ete res nel deste « opis ‘on hemor. ae conde omen rons (cheng) le ‘un proces biochimie complex Ia ere lntevin plecsicle sanguine, ‘brina anumiteeemente mineral (cals) on ms mts ani, cute ate ds conglre reid seat (10%) reid andl wa eupenecomponete | * [Congulres exe proce de vansiomar fvnogeu soli in ‘Abvind (wacoanleclh isl) sib acunen esinatid. tomb 'sctve prover inprotomdina cecal nv sub mjunes erat rina, nacre defo oometzho ten tidineneal ce ‘supine n ost saeclemenee guste, goed rob 29, (Pl rte ram m Sine | Tomint S Plasmas = Fibrinogen mom Fibrin ‘on a Pome Sa Sera Pores Pies Reprise bem proce de congue + get In coos pl spent component Hie sing ait econ este conti in or sana fringe proombing ‘er siguin inde ote componente ame ml un race roconbian cree convert moi last = ser sanguin + fibrinogen + protrombind ‘Ser sanguin = plasma fibrinogen + rombind Pras sngu obj pla eae agli pe eticonguen separ erent fig pe cena. Cigar se eee 6 ‘minute i 3000 myn (taint) rico ofigh fk ece entire indonan. oi prea arse I temper state ste conc abet. Seal nal bine pi hans stp ae ‘wombs. Se sigan s rein sub fra un id lr, clos ee Ir su spi canoe te comport pecde biochimie penradazarea majors compontatlor biochimice sanguine (csi putin fivnogenu, Ser sai plasma? une ol sain oct conor sg a Pent marea majorite componente blochimice,utlizares scestla Precint o sere de impediment: se recolteaa un volum de singe de ‘pronimatv 3 oe mai mare decdt cel necesa, tn curs congue fl ecolti rombusului aper procese de emalis nhac de mete, ‘eomandat pent eae tip de aralizhs ules fe a pas, Anteoaguang “Ancoagulani sunt substan chiice care inhib proces de coaglare © gel. in functic de mecanisal de aclu, ancoeglagi se Slack {n: Blacan a lnior de cle blocang ai rombokinae! bee 8, Tabu 8 ‘Auticonglan Clasifcas, exempl concent da slice acl angle ‘Coma ra pease | Rag ge in ew Sagi ae Moco iit de | Fs ea Tomer ang icodanisoeraccun de | impo gs sti DTA) ie __\eStia sas Uilzaree oxaltior ca anticoagulant este resins dtr efeor > durtt de 2-4 ore 3 reve Ia valoarea normal In apeciatv 12 or affine hhepatce determin revenie la stare normal in peioall de tp al Indelungatd tort ne! metaboint reuse ~ enimalepoligastice ‘Mieroorganteme Absoriie Poliscide << ace grap oats <= Singe aminale Cc ST Poe La animalele poligatrce (rumegitoae) mi inresitreaal 0 eretere Posiprandials a glcemis, deomrece sub sefunes. microoganisncor amine poligiuiele igerate sunt aneformate nacre 2, glioogenotizs hepatic, muscular anv air fst Fosfor a Fovfolucomaed 1? SS" o-5-7 _lcogen _slucoro 6- ffs’) HPO, Siege Gc 3 glconeogenza (biosineza glucorl din compu negli) exe fooviaal de coriconteroz, glucagon, homons! de crepe, fet medicament 4. glicogenagenczahepaea Chicos SS G~6-P EH G-1-P mas plcagen Dozarea slum, in paced, prezind Important Sn epiturca ezechlelesorvente le nivel acest eli metebolce, eonsiaid n indicator uit peri prognostic! si diagnosicul stor de dla a snimalele domestice, pen fen coeate cu sarea de ctr, ria pent ence eae new cre ional Reglarsaglcemiei exe sub dependent neuchermoosl; homenit care inervin in: procesul de relae runt dnulina (ol hpolccaat), (oo hiperlcemian), ACTH (oonmonaladrnorortcotop) 9 hormonal de crejtere (el biperpicemian) aderaina trexing 1! hidroitrot Dozares geo se recomanét ind ananaezs sents: com, pallu, poldipsi,afecjunt hepa, inset a ghndcor sprue, afecjuni ale glandlor soparenal, alent heptce, a” subeei iagnastcat cu note pareiral Meroe toe enim (ou hexokinase ot Exprimarea vaorlor. Gicenia se pote expr in mg. glucors 00m. singe (mglcL) sau in eal. (Immo. = mgd. x 00855, unde O55 ‘eprecn factor de conversa ligrmeor nail Princpinl mete. loses da singe, a prezega oii, a medi de Acid acetic, foreazl o colerae verde alba intense clr ind — pata geo. Probe biologic. Singeitegel veo ye antcongulen de peeat Na Ghibier af gicsszey plana separa pin centage tdi dap recotarea singeluitioh 0.2 ot hur se 1 100m), solu ei wicloracec 6% sone solu strat de acid Bennie Tebnica. Se relizarh urnitomea schema expetenens: Reset Ta [Rates ar ‘da [3 a to Slag a : Salle tan pace gs oa Acid icone re | ty to ire Fla pt co : Sot clan : 0 Soe meor Pa o-Tolaina 1s us ts EERIE in Tia Bate aD i ie aGiC TOT (Cote, Pentr deteinareaglicemiei ve aplics fade cnc: Exe ‘mg alucon¥/100 mL. singe = Baie Eaow unde, reprcit concentra etalon 0hmgh Inert reat War a es oa Fete — 70-110 Chine os Pas 120 Borne” 13295 Soboan~ 199-107 Orne’ 30-09 fore 314-20 Sime ae ‘Canide 65-118 ne Velort eric. Ose8dere cu 3,33% fi de Valorie minime nomale, ‘231 eretee de sproximativ 8 ori a pragul maxim ot induce nae com. Hiperaicomie e const diet zabart i elec o hiposereie 4 insulin’ ere pancreas, intoxcai, nett: hope: boll teen ezehillre endotze (hiperecreie tian, hpeisrd, supeain, dministncen de coniosterii, ses. poems oi: ld dete, ce snes, dito spel poi ope, ses Gace eae Sony es ens earl aha La teow, popes fal ese Sa crs rae el rae ea iiseorpainler lomisie: [somes tik posi pw tet gue Inca tipple et Sgn ves pel hen tn wih pons abn sn oe oes ee coma connie cocunis : Hipoglicemia la rumegitosre trebuie interpreta difeit ‘fa de eet pi: Exe ncaa co var fone oes ovine La etal crs Seep can Sathish) ter ge oa fo Cleat mg gluons = INVESTIGAR®A LIPIDELO? = DDosare idelr din ser se crn in conf nlc nae inp eu consece psec. Dah send pests heer Inpineo invesgare primal a seri lacecenmesinod Seka et peste noapic a foger. Fomura la toplist sree {prez chloe) eu mena pose! ose tee ns foal cl ee vata deo Aperchlomcronemis proves hese eee ‘ane in interval de 2 oe init de estrce robes Dede poset 4a “sive enzineler implicate tn" metbalial eulosicenlee ‘iets = a vce pk pee Shlomi srs imps eel ci a et Prin dzaen olesthi gicesdkr Ingen sigue ipso cu dabet abt ion, Sidononsiderat rain © cis ob biomolecule Tet 8 eu fee avn epi ee i ‘laliglicerai, Prorlor. Coscia Uplelor totale din er sanguin CRs exc ng lide/10 a (mpd) su faa Urmtoaelor aspect blochintos 4: digesta ipidelor exogene ‘llcerde past monoglecrite Sesfepide Se ‘leone ‘Poncreatict —colesere! |\mcoasa olesterlentericat ines fat tut apcs comiiront 2. Iipiel endogene cre deo pin plans oo efcunet su or de complexe Coes tituite din canta variable de wigheenae, cane olesterol estrifiat, fesfolipde iosinetone de fiat in plasm. upd comporarca a Shicarfugme gs la clectoioeck, "popes see ctlaicront popcide de dense forte joast (VEDL} ipsa ne ct ‘esi joes (LDL) lipoproeide de densa nl HDL) (eben El rine tou VDE) ple edn et 0) Lipide det a od) Sen tages incon 2 2 2 Sse oben Dac Ta adh oma amas 5 Ber lafstee de adh = ome (Clea. Se cletec pein dup reais Ente Exon slide tott!100 mh sp = Foe, Ente Ene4 Deoaree etalon! de, 700m oink % corepunde In 1000me lide serc lta fn formula de ale seconde sy loaane, DOZAREA COLESTEROLULUI Consideretil generate. Colestrul es un aloo police bose de ‘sproape tate esuturil, in special in feat bloiniera ste pote realon ‘a nivel peetell intestinal, glandeor supracoale, cracls, esicoleos placente, Colesteolul indepinee funeiesenfale in omgnism: component ‘membranelor eeu, precursor al aciilor bilan, hormonior mero vitamine! D. Dsoarce colesrolu se precint sb fom ‘ibe ‘seifica, sume acestor cone colestrll tl Nivel colesterolulul sanguin este dependent de echilirul reaiat Inte: snted~ depcitare~ degradre~ complexe lipopotec, Shc de novo ‘nfleat = a TC Colestorol sinetzat, ‘eurahepaie Formarea HDL, VLDLLDL = Converse ix ‘act Bitar CColesteolscretatn ils harmon ste, window. Esprinarea velorilor. Colesterolemia (concentra colesteluul tn see ‘singe expr in mg coleerol/100 ml. sx sanguinea) Principat metodel. Coleterolal liber qi cel rezlat din complexle poprotic, sub actunes precipita acl seailic foreach, In ‘media oid i tn prezena enidrids seetice, o cuba verde. Inesiten ‘lori enue este proporoalt eu concent in cles. Probe biologie. Ser sau plasm reste 13 ore postprandial, ‘Renctv. Aci acai lai acid slr concent, anid acetic cid sues 12% in acid ee, solute etalon coertes 250 mp6 React (aL) aijena rutesie Scam Sala tain 250.4 ansershacit Solsieclin fects | |S Sn cx tn: a a Re pa RST imei ‘Foie ST ape Op Bc DET Catt Secaleaou® colesteroeia dup rei: Ett Be 1g coleserel oa!100 mi er = Ein Bare DOZAREA TRIGLICERIDELOR DIN SER sideratt goneraie, Tegiceidele (ciscigicerl) sunt estes ai stierluicu efi aii gra sot neutral Tloerdle se a eporiate in adjpocite, unde altiose read. energth pent, comzisr, maven valrion. Concentaia wiglcerdelor se exgrink tn mg ‘eigen. (mg). Principal metode Tigiceiele sevice, prin expire, ya t berate lier sl seule “aeislor greg eomepumatr ceo tat Ia lum procesu de srenifeare xe od, prezeni acd perio a slide frm, cere ea fviirin tn media aid former or eras e condensare color a op crc, ‘itesiaten eur rerutate nd ‘ice proporjonal ex concen pide Probe biologie. Ser sau plasna reali 12 ore postprad ‘Renctiv. Sole KOH 49 in leo! absolut, aoe MgSO, .7H,0 3.7% folic ccd sulfuric 2N, sluie mtaperodat de soda 3.10°M prepa ‘extemporsnco, sole HC! SN, sluje feniidint 44, solufe elon Iles 109%, Tebniea. Se realize wrnStoures shen experimental:aa rae ipeioniconi pl ec cng gop = rt rec ae sts Monge Roehm a any pee TP a Sotelo kent 10Omgs) | a ~ anal ie hci te, Rinonai) ee cna ec we eee ermine at dna it, aca ine} Need parcel, ones hee zi a tere cuconos Sr cetcann or fal rac se cer sete a 7] 7% air sof) tn saan ih ct pe ann te 0 to id utlizszA plasmt proventh din singe recolt pe Tore sea ae | Meso ena | a 03 a| ticugteg (bdr ese de tose © ala et ” Repost Lirias Hipolipidemie se constaté in: infe = : in ews, hpi, ani a Ss Nee oe tomo a —— TS] BA ted ss a teeptcosee nee ee a sen ES olen cs terse See SE — 1 vege ag ca Sense he 8 Cote Scat lente ip lai cree tig tt Rm es aroha ee o,f nue ce el ra tse ma ee oes ic eu Nowe peste tay ps poe has nargtrueidne eo eames "mcmund ine eevee pops eres Sar modih il more dr de plot ol a eae: tance cu. ‘homt nek" nce pla Valorite_ normale (mg/100 ml [Pestilicriemie, Viana C¥ heperna eu un fet inves, determiniind (See T Vnte ae TCO Maia MO Nieto Cabaine 300-4) 15-150 _ etentrea realtor Bovine 300-400, 80-120 15-49 ase htinceride Ovine 300-400 60-150 - pe eninctll_ Gs Stine 300-400 | So ia0 2 E Cine ‘o-oo | tomo | 10-10 acs 360-600 | 0mm S60 Prat 300-700 | exit =i foboin : seat sn200: Source z moto | mari etnies | ieee 2 so11 as 2 | 2 ria | Miptcne cinder) ~ se rah eect fence tata o ace de sre impli ds a Tide (e exempla ipoprteintnany hipetpaprtseINVESTIGAREA PROTEINELOR TOTALE $1 A ALBUMINELOR SERICE Poe 9 are, Pts late pein stn Toney 1 ame seni Ceprosinty 196 Gn resale Feces dA sic: ot dere pe TOO ae eal Sie le i trove te npc eee Tima tie Fea, sistema! reulendolal (emesis t limfocts)prcum pide ale pata Pet ce “nena “alee petal a iam _ = cimposotute i Reticuloendotetiod (= lobutinele) Alte cette gt ~unele lipoprotede Teswtur) jpn a eune, nen ica mete Gtsefline S edianens (uml ta chal) tres ce il ent lan chal cot Pd: fii pre’ anlinateplasn conjindad gf Stop aici seme si, conulopt, das, pied in preute, sfei renal sau heptie incisal ret i conti apc ool sf el oj, ‘epi dexevany) ex cela sinter nese ane A laborator penta septs stabil on aga Invesigies prota ec se poate fe pen: mete de dogae a froeincor tle (gnvines, colo, refscomaric seo 12.2 im) meade de separate (secrofoeh pecpiar Re iemun, Seacie,omtowe) gi pin meade immackinne t ‘adicimunotosice DOZAREA PROTEINELOR TOTALE DINSER Principal metodel Priel sce reacjinea eu sinwile de C4 tn media slei, poducnd 0° cloaie woe tele inant et ‘ste oarte unporant ca inepetarea rel ‘eactv, React bret 45 aa esi potas ili Om. {elae NAOH 0.2 oa 1.5 g ult de cpm 08 Klee ag os b sipletcaa cu NaOl a pind la 100m ~ soa cuts hea Ge, ie KI 05% in NaOH 02 Nin apc Isle nc hee ‘pli talon lounind sei bovind de concent 108 Filnic, Dozre prowialor wie ve poate elton cy 0 sluie elon ‘thumint serch bovn au eao cub de ets aba cocoa {ehtmn de concent coring ave | wi 10 gL. Seelanct ras experimenDOZAREA ALBUMINELOR SERICE. ‘Albuminleconsiuiespoximativ 35 ~ 50% din wa protenlor setice In animale. Exist 0 core deci nie turnover pote st ‘mdrmes corporal a aninalelor De exerplu inp de injundae a eine fs de ae aac 19 ae Drow ee pela tet Fr Ge nats moet cn nal mk 58 da winines o e prot 00 m= oo ‘omelet sian de dius a Esprinare rarer Alaina once abs a se indir gabvmnino100 eg ubuminedL) ia ngaternea > Meola crt clos alae an sins eres tv ‘Pinca Send Alanna Soe eee nae sb comer 0 pera, in gees hip ns fees Brom eo (YAO, preted cS SH a sl de eau de cocenai case he Ta ee Sloman ce ree eae ret opal oe conf nw ces ‘ooze abun Probe ble Se neteni a 50 (9). See Sa Ga React. Salsie verde de toner, sli ala Bild, aye = es Sig ‘talon nid weave r oe Tonic, Serene tra hens expen 2% % 8 eae [Pa ete Ta) } ie 3% Sea t | 2 * in Fea tegen : FH A : eee | |g Beaty o | & COO iar ca aga T= en cars elon pent rob elit se devo et de EP ), Sees exuncile a totcolorimety 9 poise repent rae E =). Cunaseind extinein probe din pac sufleient de mare modifi ptologee ale ws jst organ sv gap 8 corzame Tot, uneori, dei o enzins ponte iden’ sce conde ne ex inst intodusd in deere unui ie uc cron ene Un cxempiu il consiie cele tet enzime.apelice feat amines, Sorbitol dshidrogentza i alaninamintranseaea (ALT sau OPT), Princle out, arginaza si sobitldehidrogenama src, snl spsfce lesrlor Anepatic a cle mai mute spec. ALT sau GPT, dy In tele animale are 9 specifics hepatic mai redid, se flosete caren in aberaerl ini 100, it "fers vd #0 Hoy fo cr cr a Formaren ionulsi bicabonat care rece in plasm reflects tcparescloruui la reglareaeculbrl aid-becc al plane. Closet contribu gi a replarea enald a cchilba! aid bse pron mesons seminar C17 HCO, care se pevee lave ler, Exprimarea velorlor, Cloremia reprerints concent clo in see ‘sanguin ise exprima in miliechivaleg la iy (mEe/L) sa in limo i lina (wmoVL); Im e/L.= Imo. Docareatonulu clorui se efectcezs. tt prin mete specroftoetice ‘it prin metog rimetise 9 retrectometion, Princpial meode. ln de elor se tireaz4 cu azote mercuric 091M in rena dieilarbaonei ca inicatce (ind, Puactl ial al tie este {indict pin aparita une culo volte datorteabori unl mie exces Se Jon merce! gi de indicator la supafna precipita de lord sarcach, a ae Hg \ Ind Ne Hg +20 Hg —» Hgciy He gc ind : i Hw) aes a os 7 Probe biologie Se uilicnz ser sau plas sanguin eclndye EDTA, ‘React. Sie oat merci 001N, ele etalon Nac, (100 me, 1) dienlearbzond 01N Tehniea Se eaizeazS wraioaes scm experimentelé ‘Reaetii (mk) Fab Baie) ‘aa Babee acomeon| | Saucier” | oy | (Catt Perr alevareaclorenie te spit lin: Fomoon ot = Version Unde: Ypnpony volun de ant meres lia irre pre Vein -olumul zat recat ata sle = concent etalon expat in mechialeni airy (100 fi a rt ay ean aa ogi a “ipercloreni pan pot linea eager, fein renal (lininre Schait), penubarea ecb acdoise (eee restora lor ia nivel tbr ren pen a compen imines Sob bicarbonate soci cu deshiratacen organism ule fen endocrine care afecten fie eal‘ipocoremil se constath nurs gastrointestinal eiminae pin dare ‘su vomamente), intoxicaiasciate cu moblsrea clru neice ¢ leziuni Ia nivel tublor renal sport alimentr ‘Suse de crore. Probe hewelizate,hpetipenice,hiperpotice. Medlcmente care modificd nivel cloraa seri: Blcabeniul (sa ae snfacide), duretcele, laxavele induc hopclremia: Tatorento, ‘rlungite cu hormont minerlocorcoiai androgen, meticamentle rare fonjin lorurk de amonis, coletiami’ precum qi medlcurentee fatieumatice, perfil cu’ solu hipetone de gueoal sau, mantel detec hipersre DOZAREA FOSFORULUI ANORGANIC DIN SER SANGUIN Conder generale. Ape 8% nl cnt ermal vere ep inset fo iio die ee Concept our ee rend eh ‘special ca anioni al sisterului tampon H;PO,/ HPO,*. O mich parte din ‘Stra plat vege et & pine (incerta pemmiet «Oxbruki anorgis eet vr cee ingots did sporala eesea ges (este Auctoee de linia Tally rnd cecal Soa ems “aia eect xt ml dee et evi {dul De temp, ncn de al mar sei coda os tre nen ai gt ae ‘tora elimraren Se stub dus 75% pfx linen, tem deft ap Cina Sn sone sche ezine fens etn crt pata pes Se Dea me oes Ce a bf fc psn ca oor one gee ne, fe cay acini de tur ci pin arn nls Assos Ki a oe rect aie Jol, a pl cle Visas sine hari tl fs Bini oxford erpon wee pl map: eal sarah att Laelia ied npetoes ana, ai lee of perdi atc Re os el ft eee eal rel rec ‘ol fara organi. Con fos et tbo wat opine cele pani med ces a opens procese bioshimice, Fosfor ae tit rol plats elt gl fcions, find ‘reat su form de ete! n cmpocia uno biomelecle important sca mel, fofoproedle,festolipidee, comps maser, ee {stort i czlr, subsoja ‘sous. ntervin alt de ae subsans snorpaice In relirea homeostaze sido tuo organismal enimal, Exprimares recltaelor, Fxforenia regrets conceati foto set 30 expind In mg/100nL (ng/L saa mmol, Convers uniifle sheuces nt le anol 028 mgm ocareafosfout eee in special pin mode eaarnetice cre uilizesl ea reset de exo molibdal de amenii, Dien Gre metoge floite in laborwtoru clin ete dette til Ge agent reduce flit. Frincpal metodel toni fon eejioneah cu mold de anon fn rezenja uni agent reducer, in medi alain forlnd un complex de ‘lore ales Intensities cull emulate ete dct proportional co ‘concer in oso se esineusAfoometic Probe biologce. Se vile se sau paseo ecolathpe heprn,Ciza ‘ovata s EDTA tered cu meta de ozare Reactvt. Soli ealonP én, sla reductowre de hidroxilanid 03M in medi de ai auf care oon poiviiprldon,slie N¥OH 4 Teinica, Se relies umdiouea schema experimental - a seca pee ficients | oS || | == = : Sledge ou {sao —— ee Dap ite a se gine a = Cleat, Se alse osteitis cd foeltOmi sr ™ aye, AInveprterea eater Yr normale gmt)Suine 53-96 Cline 246-52 Piset 45-81 Sobolan 60 Hipefosforemie ve consat Wap alimentarensgea insulin renal, Inaufclentt partoiian, duh facturi sau tumarl sotse,hemolil Inursvascalard exces de vitamins D. Hipofesoremie, se consi tn; por slimestar_sedat, hiperfineje aratridiant, dire, rats, hipoitaminos Dn tmp let! a va ai nto ms, 23 nL OHI ier sl" Se a ul a aS sins ale era ernest ez ania 10 ‘se aplicd relatia: = . Baie ESRe100 mL = mM) «F 011-10 nde Vm vom ead ais connate ard \p- voll dc sls connate ee tral decor el lied als tne canine uC corepde ‘mL solugie NaOH IN; ‘ 10 fort de muliplin ecs po fe 100 i ‘ucru s-au luat 10 mL. tapte). a pee Intepreares rection Cost In cami a ei vai finde pada deca dees sa Ul at ese a nor anata conposta Heciie # eete ot ‘ool DOZAREA LACTOZEI DIN LAPTE. Princip meade in prezns feo cul lfc conenind, lector fomneazto color trunk Intensaea cll ean oe dg ‘operon cu conn se etinean otmett Exprimareavaloror. Cont tn lacerd al laps se erind ig lector 00a lp () Probe logic Se wera culate prosp deze pin cetsfge est Soe fol 9%, wid fuse cnet tte clo oe et. Tenis relizeact urns shen expen ‘eet a) "rst iti acc fetectiog el Adalat omen Caled Ete Ene Fania Ene Interpretarea reitalor. Aproimativ 60% di necesarul de ico sh une vel nlite wliat penta sites loz Sonat gn ‘azul in cre sport limes ete setzut, gucoennectord ete ssgsal ‘rin eucoosogened. Apoal almentr iin, corelt cu rode de Factors, constitieprncipla caus instar etre! de lactic a veh ininuare conti nator al lp paren cd devel ‘ntre diferitele mecanisme de compensa apocozi este pea indeingn ‘loti = DOZAREA VITAMINEI C DIN LAPTE. Yramina C (ail 1- ascorbic pect i ot hie 6 fesuurle orginal Cee same spe onto amin C din gucoe, afl ck nec lena exe minim ei ‘cea pul geal epsom inal ail eye ‘poral alimentree cba Rol cs excoriation pevoad timp, sexe complet ida cial antic es get er pte, iersenind oe ‘seid xidesedure cara In siecle cum a oe [rote a dlelrtnpove sep radar bess De ences, Inevine tn vec pn care se gered rp abot! Bled tubsrt orgeml ree Una ine cle a imporat text de doula Ince pare cdl asrbicee ce 6 fore 8 hidrovirtn! oan nisl ites! prnagels Neat fancl expid aprile leu sua’, Epes onus #4 capi ex tof cl de vinnim © Fears ses ba lin a eeclmio arael eden) irri fe tou patie imine! CPerioeele de tes, noite cu sed rec de cntlaw ea ces Vin Recon oa coma ail coe exe sia cu pea ik de nitrozamine care sunt substane cancerigene ce se formeszi din nitriji $i Amine. Vinmioa Cinervne tn prove de store, wep fab ree ‘vitamninei C tn lapte este foarte importants pentru animalele” sre, eiror res asus epee on cont ltl it xe‘es. S- const ck primetle sfbaice ade tn captivate au un nivel sere sein in viamina C dort sremisl Gecsar’ crest ie Satsclanine simpli de vitamin C), Sind mal susceptbie I fest Acestora, cf omulu le ete acer un apo erect de ned neat Principial metode In avedia acid, te Aclorfeolindofentul, “acidil ascorbic "se onde Ie ari schidonscorbic, Rescivul de doar (24 dlorfenolindofenll) a fol at ‘agent oxidant de cao ae ee ion @ on 2 4c + 26 dotnet wm sell + 26 dlr ‘awh "rma srt ‘homcorbe Goma ead) ‘het bear Exprimarearecutatelor. Cot in acd acobie se expan mg aid ‘sscorbic/100mL (mgt) lap a in mg 1000mL ae, Reactivi. Sole HPO, 2,5, sale ci ascorbic 10°N (echivaenal ‘7am al acid ascorbic est 76/2 8, soli tampon ested eo, Solute 26 dielocfenolinofenel 10° Nia tampon fot, ‘Material boli. Sewlizsas robe de ape prop reco ava lume de oxida sav detergen Tebnica > In paar Eseuseer snk 2 mat, > ‘Sadat bagi pct x ptt aden 2% pl a iit comple tener > Sexe veut Von de el zat > Dip omopssire amet se > ln capt e porn edt fleas > Setiea rot eaten 6 arid ple pu nl ca ot ‘ar peri ip 0 denen > ‘Serene volumal de rc Vp wat Se ‘Determinarea factors de core a sla de 26 dilrferolindefonol nr aor sleeves need 10m al cae een sd ci 1 Ny > ‘Seta. iret] pt Isp clo co ‘el inp de sce ct Preenia 26 Sem rac a iV ‘ (ata acon decom vacvacln upl: Yopome a ua aid wea, eins nm, in 10m filet eet ee “i act ontc ~ VprPONOMES setae 1 vote WP word dccociedraciviliedeare Spee ominp nc pot acttees cies ll weed ei nortan rete ge detenink => de lap cre corepunde I 10m ra 20a tape (20+ Viet con. a 1 a tn om ge 20 x= ——— mile 20+ Pow ce determi a cantata de ed store conint Tn 100m. lap: (20 mgt mere 100 = [me VPN saree 10) 00 — fein merrier, exit a cd la a cfr scan eri vei ‘tetas ie plete cin Co otraNOTINISYYD V YOLLIIVNV RIVZNIALOVAY dq YOTIDTGNI VaNVNINWALAG 24878 00 ari00s pe TaD BURT eat 2» sun [sp _wmn|_opospans| me sn RN TRE used ewayp coud dumeou i gms sel eo ezede., sr meee =p = 2rd 10 901 pe mao gna ay Hud pe qumusband wipe giterd a 7 a eT ee, | oe STA a FT = {ru oma o eevee oj aes was una ead rea, | rar eIoe fn pia”, “cu olen, toa ie eect sini 2 determing anu nde ‘cue reflect ompocitachinief,gradl de neste, dred de porto Inwoducereaunor metade fieachimice modeme "in setat ‘compozjeicalitstve gi eantiatve a gesimilor prt: eromatografe de ze pent ci grag HPLC (romaogrfia dina performant high ‘performance fqud evomatography) pentru taligicerl restr aia de are a matodelr chimice de enliak. Tot avnd in vedere dtten Inboratonrlr, pesum si costal siz, se vllzencd ned detennones {nde de spon «Incl de aint pnt cca DETERMINAREA INDICELU DE SAPONIFICARE Iniiesle de saonifiare ()repexnth cantatas de Hidde’ potasia ‘xprinat in grme neesatd pee sapnificare ul gram de pists, Determinaren indicelui de saponicare permite apreieh ape nese ‘molesulare medi a acizilor past careined ta consitia giceidlo, valores indiceui de saponifiare vaind lives propoint! ci naa ‘molecular aciilor grag consitieni Ast, alo erseue al incl de saponificare indie rezena Ta structure glceielor «acallorgrgt cu ‘mum ne de atm de earbon eu mash molecular mit), fn tabell 7 sat ‘rezent ind de sopoifcare parry cite iim aniae wees Exprimarce realize. 'odcso de aciiste 32 2nd th meXOMig eine Indicii de fi ug ci desl pening ogee gl nae ia po cae aa Soa st pane [aaa eee uses ee 3 tase tae te Pate tee ati ie [amr thats Ses el a 1 [umes eat 1 |e Princip mete 0 cnt cts de sine s saponin fecsrs um vlon eax isin de bdo depot oe Ficronié! de pas cst oe vo soa Se id ae in resent de eel. Canes Se Nod depo ia Sepoifcre sedi pres goer tn am de Nevo Sou aol de KOM salud HClO, eat TWinakool te: Teli Seren vores chins exesimentl dr Plena ed: ——————) Tesi at rar ‘ie 5 pane Sate KOM ‘at oat. Ta bare se de use ¢ a0 SATO RT| a Se ers RSTRT ae pT ii Oe CATR psa | sn emt sil nn el pr a Cate, Dien Vp rerecits volume de sous HCI OSN ace noulizeaz sola de KOH consuat Ia esis de sspnieae. Aplicind mula de call din volume Yes x20 = rep) wet mB unde: Fy = ford core al wo! de HC, [Npe~ normalisten soi de HCL, mg go = iin gram al KOH, “Transform masa roid de potas in illgrame se bine: san = Woe wei N mE ‘Cunossnd masa de grsie 0) si mass de ios deposi exit fa riligrame cae a peipa la sapniiae, prin pore, oie india!™ de saponin: OmVp rca nage! s beDETERMINAREA INDICELUI DE ACIDITATE Grisimile se degradeacd pin. procetul de tre, cespectiv sutooxdere In ua acesorprocese st eibera cei pra pode de Scindae oxidtvd al acestrs(aldehide,alchide-acii ee) oar sonfnk, srtsimilor mos st gust neplcwe. Detemninareaindicelat de aide ermite dterminaren gradlui de pospefime al une! grsimt, Deonree onjinaa in sci gray este mal mie de 01%, penta 0 grain prow, Indicele de aia tinde cate zero, Penta cal de mine de fone ‘rezeta un eorint in acizi gai Ube de pln la 3% ve admit nde de cdi c valor mai mar compart cw indi de eid sorepunetion slo aris. Pentru, date prezeiel cir ibe Bae’ eaprea, ‘aprile capri, indicele deat est mi mare de zee. Determines indiceli de ciate pentru weil de malin, unt gi grin! dn lpte, Bihon Mo Duberingeliok Chica Ceo hn alert spect Pppeses, Corlatons, Ed. 4B. Lippincot Compasy tose, ‘Specie | alo | Specie] Valet Spa |r » citulans 1 Coit Biochimie cnish Bd Dace Chi. Napcn, ermal normale normale 1938 hal z > LX Dunc KW: Prac Vein Laboratory Medicine: Clncal. Gibalne [78-115 [Bovine | 45-73 [ovine — 37 Eaten, lows Sut Uiveniy Pes AMES, ia one TA Gzrine | 30-78 Jsine | 45-150 [Puts | to Sp > SisortS, Pop A. Kader, Manual de labo cin vtvoar, Ea ae | 65—118 [set | 70-110 | otstn [477107 ALL 90, { ied le mg). ecb lete 5 Chee Chemise and Management by Latrnery [bale “300-400 [Bovine [500-400 Oviee [30069 Methods W.B, Sander Fa Phyadcia 991 OF Cepsine ~ gu Suite, | 300-400 |Phous | 500-500 > Dog, so — Labora Test Handbook, Lexi Comp. Ine, Haan, tine _| 460-640 [Pisa me 1990, Tig » sri aie eel, Chil Bashi of Donte | ag] —— a Aviva, Acad, Press, New York, London, 1983, > Sie Canalis M.Srbon dnea Pp ~Bshinle aah rin esi metodlogce, Ee. Priatec, 2000, > DX Llunmer~ da Incodction io Practical Biochemistry, Ms: Gram — Fi I 75=150 [Bovine] 80-120 [vie | 150 Hi. Comp. Londen, 1967. | ]Ssprine | 0-130-|suine | 90-120 Pooet | 225 BP > Repo Natalia M. Serban ~ Meta ns de labora Biche, Cine | 0-300 S88 yoIL Il Ed MunteiacLeda, Constants S000 > M, Serban &."Canpoane Em. Tonesea, ataline —[52—79 wap ochinia animal, Ed Did Ped: Buco Caprine | 64-710 38-56 > M Serbon Princip ale diagnoses joie | S427 300-17 eterna, Dd. Ped. Bucur 1982 > Millard MD, Tvedtn ,D. Turovalé G.H, Smal Aina ~ Clinical |Cabalne 35-37 ap Diagnosis by Laboratory Methods, Ed. W. Saunders Conny, has [Caprine | 29739 5 ictie | 26-33 Lag743 Cabatne 325 6H Capine | 24-83 : Caine | 21-102 aban 21,8484 Cabaline | 226=306 Caprine | 167513, [Caine B66 Onine [364-357 Past | 174g Sobolan_| 32862ILLVDINAWOD 16 1N91V9 4d YOINHAL O606DIZTOES LOS6OITIOL “PIT ONIGVUL CNV DNIGdTHS OMNVLLVSNVEL