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    This document provides information about analyzing sucralfate, including its molecular formula and weight, in blood, feces, and urine samples using high-performance liquid chromatography (HPLC). It describes preparing the samples, the HPLC variables used, and the expected chromatogram results, with sucralfate eluting between 8-9 minutes. Keywords provided are column-switching, radiolabeled compound, and plasma. It also references a 1982 study on the pharmacokinetics, metabolism, and binding of sucralfate to gastric and duodenal ulcers in animals.

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    Sucralfate by HPLC

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    This document provides information about analyzing sucralfate, including its molecular formula and weight, in blood, feces, and urine samples using high-performance liquid chromatography (HPLC). It describes preparing the samples, the HPLC variables used, and the expected chromatogram results, with sucralfate eluting between 8-9 minutes. Keywords provided are column-switching, radiolabeled compound, and plasma. It also references a 1982 study on the pharmacokinetics, metabolism, and binding of sucralfate to gastric and duodenal ulcers in animals.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    122 views1 page

    Sucralfate

    Uploaded by

    Papaindo
    This document provides information about analyzing sucralfate, including its molecular formula and weight, in blood, feces, and urine samples using high-performance liquid chromatography (HPLC). It describes preparing the samples, the HPLC variables used, and the expected chromatogram results, with sucralfate eluting between 8-9 minutes. Keywords provided are column-switching, radiolabeled compound, and plasma. It also references a 1982 study on the pharmacokinetics, metabolism, and binding of sucralfate to gastric and duodenal ulcers in animals.

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    Sucralfate
    Molecular formula: C12H54AI16O75S8
    Molecular weight: 2086.7
    CAS Registry No.: 54182-58-0

    SAMPLE
    Matrix: blood, feces, urine
    Sample preparation: Urine. Centrifuge, inject an aliquot as described below. Feces. Ho-
    mogenize with five times the volume of 0.01 M (NHJ2SO4, re-extract the precipitate with
    0.01 M (NHJ2SO4, combine the supernatants, inject an aliquot as described below.
    Plasma. Dilute with two volumes water, apply to 20 X 4 25-40 ixm LiChroprep-NH2
    equilibrated with 0.01 M (NHJ2SO4, wash with 10 mLO.lM (NHJ2SO4, elute with 1 mL
    1 M (NHJ2SO4, dilute the eluate with 10 volumes of water, inject an aliquot as described
    below. Apply up to 5 mL sample to column A with mobile phase A and elute for 10 min
    then backflush contents of column A onto column B with mobile phase B. Elute column
    B with mobile phase B and monitor the output. Re-equilibrate column A with mobile
    phase A.

    HPLCVARIABLES
    Column: A 40 X 4 25-40 \xm LiChroprep NH2; B 250 X 4 10 jim LiChrosorb-NH2
    Mobile phase: A 0.01 M (NHJ2SO4; B 0.8 M (NHJ2SO4
    Flow rate: 1
    Injection volume: 5000
    Detector: Radioactivity

    CHROMATOGRAM
    Retention time: 8-9 (after column switch)

    KEYWORDS
    column-switching; radiolabeled compound; plasma

    REFERENCE
    Steiner, K.; Buhring, K.U.; Faro, H.-P.; Garbe, A.; Nowak, H. Sucralfate: pharmacokinetics, metabolism
    and selective binding to experimental gastric and duodenal ulcers in animals. Arzneimittelforschung,
    1982, 32, 512-518

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