TRNG I HC NHA TRANG

KHOA CNG NGH THC PHM

HNG DN X L S LIU
THC NGHIM BNG SPSS
1. S KHC BIT C NGHA THNG K GIA CC GI TR
TRUNG BNH
2. PHN TCH HI QUY

Ngi trnh by: Nguyn Vn Tng

Nha Trang - 4/2013

 VN T RA

Th tc x l thng k cn p
dng l g?
Phn mm h tr nh th no?
Thao tc x l ra sao?
Cch c kt qu nh th no?
1. S KHC BIT C NGHA THNG
K GIA CC GI TR TRUNG BNH

Khi biu din kt qu th nghim

Thng tin cn c trn bng, biu gm:
- Gi tr trung bnh
- lch chun
- K hiu ch c hay khng s khc bit c
ngha thng k (p<0,05) gia cc gi tr
trung bnh (thng dng ch ci a,b,c)
 Bng 1-Nng sut sy rong nho ti, nng sut chit v hm
lng phenolic tng s ca rong nho sy nhit, sy lnh ng
v ch long
Nng sut sy Hm lng
(g/100 g nguyn Nng sut chit phenolic tng s
Loi nguyn
liu ti) (g/100 g cht (mg GAE*/g
liu
kh) cht kh)
Rong nho sy 2.21 0.08a**
nhit 7.62 0.11c 1.30 0.02c
Rong nho sy 2.26 0.09a
lnh ng 11.02 0.05b 2.04 0.03b
Ch long nd 15.99 0.09a 13.58 0.01a
*GAE, Gallic acid equivalent; nd, Khng xc nh
Ngun: Nguyn v cng s (2011)
 100

90
Vitamin
Vitamin C C a

Seagrape with
Rong nho sy nhitthermal-drying
80 a
Hiu qu kh gc t do DPPH (%).

Seagrape with
Rong nho sy lnh freeze-drying
ng b
70
Oolong
Ch longtea
60 a b

50 b

a
40
b
30
a
20 b
c
c
c c c c
10
c c c
c
0
20 40 60 80 100
Nng dch chit (ppm)

Hnh 1-Kh nng kh gc t do DPPH ca dch chit rong nho sy nhit

v sy lnh ng so snh vi ch long v vitamin C (cht chun).

Ngun: Nguyn v cng s (2011)

 BI TP TNH HUNG
Kho st nh hng ca nng ethanol
n hm lng polyphenol trong dch
chit ch en
Mc tiu cn gii quyt:
- C s khc bit c ngha thng k v
hm lng polyphenol trong dch chit ch
en cc nng ethanol khc nhau hay
khng?
 S liu thc nghim
Nng TPC (ppm)
Ethanol Ln 1 Ln 2
50 419.730 399.636
60 507.152 480.356
70 524.320 528.264
80 602.112 617.600
90 614.528 619.520
99,5 222.870 242.604
Ngun: Trn v Nguyn (2013)
 Th tc x l thng k cn p dng:

- Phn tch phng sai 1 yu t

- Yu t: Nng ethanol vi s mc k = 6
(tng ng vi cc nng : 50; 60; 70; 80;
90; v 99,5%)

- Gi tr quan st: Hm lng polyphenol (ppm)

 Phn mm SPSS h tr x l:

1. Dng One way ANOVA ch ra c hay

khng s khc bit c ngha thng k
gia cc gi tr trung bnh

2. Dng One way ANOVA (Options -

Homogeneity of variance test ) ch ra c
hay khng s ng nht gia cc
phng sai ca cc nhm l c s
chn test trong Post Hoc Multiple
Comparisions
 Phn mm SPSS h tr x l:

3. Dng One way ANOVA (Post Hoc

Multiple Comparisions) ch ra c th s
khc bit c ngha thng k gia cp gi
tr trung bnh no v nng ethanol l
bao nhiu th hm lng polyphenol trong
dch chit ch en l cao (thp) nht
 Thao tc x l
M giao din
SPSS

Khai bo tn bin:
chn Variable
View khai bo
tn bin theo
hng
 Thao tc x l

Khai bo d
liu ca bin:
chn Data View
khai bo d
liu ca bin
theo ct
 Thao tc x l

Thc hin phn

tch d liu:
Chn Analyze
Compare
Means One
way ANOVA
 Thao tc x l

Trong hp thoi
khai bo yu t
v bin ph
thuc (gi tr
quan st)
 Thao tc x l
Chn option
nh du vo:
- Homogeneity
of variance
test bit
phng sai ca
cc nhm c
ng nht hay
khng
 Cch c kt qu
Nhn vo gi tr Sig trong bng ANOVA
bit c hay khng s khc bit cc gi tr
trung bnh:
Sig (0,05): c s khc bit tip tc
phn tch su ANOVA
 Cch c kt qu

- Nhn vo gi Sig trong bng Test

Homogeneity bit c hay khng s
khc bit v phng sai gia cc nhm.
 Cch c kt qu

- Sig : khng c s khc bit v phng

sai Trong Post Hoc Multiple
Comparisions chn nhm test (LSD;
Duncan; Scheff; Tukey; Bonferroni; )
cho phn phn tch s khc bit gia cc
gi tr trung bnh.
 Cch c kt qu

- Sig : c s khc bit v phng sai

Trong Post Hoc Multiple Comparisions chn
nhm test (Tamhanes T2; Dunnnetts T3;
Dunnnetts C) cho phn phn tch s
khc bit gia cc gi tr trung bnh.
 Cch c kt qu

Vi tnh hung ny gi tr sig trong bng

ANOVA v Test Homogeneity u < 0,05,
nn trong Post Hoc Multiple Comparisions
chn test Dunnnetts T3 cho phn phn
tch s khc bit gia cc gi tr trung bnh
Kt qu th hin trong bng sau
 Cch c kt qu
Kt qu th hin trong hp Post Hoc Test:

Du * th hin s khc bit gia cc cp gi tr

trung bnh
Khi khi trnh by s liu trong biu ta s dng
cc ch ci a,b,c phn bit s khc nhau c
ngha thng k gia cc cp gi tr trung bnh

Nng Ethanol 50 60 70 80 90 99,5

TPC b bc c d d a
2. PHN TCH HI QUY

Cho php d on din bin ca qu trnh da

vo phng trnh hi quy v h s xc nh (R2)

1,000
hp th 760 nm

y = 0,015x + 0,1072
0,800
R2 = 0,9957
0,600
0,400
0,200
0,000
0 10 20 30 40 50

Nng axit galic (ppm)

Ngun: Trn v Nguyn (2013)

 Th tc x l cn p dng:

- Phn tch hi quy tuyn tnh: Phng

trnh tuyn tnh: y = ax + b

- Bin c lp: nng ethanol; bin ph

thuc: hm lng polyphenol
 Phn mm SPSS h tr x l:

1. Dng Regression - Linear

2. Dng Regression - Curve estimation

 Thao tc x l
1. Chn Analyze
Regression Linear

2. Chn Analyze
Regression curve
estimation

Khai bo tn bin:
+ Independent:
nongdoETHANOL
+ Dependent: TPC
(Model: Chn linear)
 Cch c kt qu
 Cch c kt qu

Phng trnh hi quy: y = -0,995x + 548.610

H s xc inh: R2 = 0.015
 Cch c kt qu
Chn thnh cm n