Kons.

Baku
X.Y X.X (Y-)2 (Y-Ypred)2 X
100569 0.25 364881033564.694 28792170830.8964 0.5000
0 0 165266505390.028 52130246465.8308 0.0000
1021114.5 2.25 15487512118.0278 8287491097.60739 1.5000
1697472 4 1896094450.69444 15257612365.6504 2.0000
2928442.5 6.25 134091576286.694 1848941.17669079 2.5000
4591485 9 526064683576.694 24736744408.1054 3.0000

Err:509 Err:509 Err:509 Err:509

Err:509 Err:509 Err:509 Err:509

(X.Y) (X.X) (Y-Ybar)2 (Y-Ypred)2 X

10339083.0000 21.7500 1207687405386.8300 Err:509 9.5000

Mean X = 1.5833 Coefficient

Mean Y = 805191.8333 SLOPE 400958.0994
Mean X.Y = 1723180.5000 INTERCEPT 170341.5093
Mean Regresi 1078481291278 R2 0.893014
Mean Residual 32301528527.32 SEP 179726.2600
F 33.3879
Sig.F 4.46E-03
 Pembacaan Instrumen Aktual - Residuals
Aktual (Y) Prediksi (Ypred) Prediksi (%) X.Y
201138.0000 370820.55901 169682.55901 -84.36% 32067.5
398662.0000 170341.50932 -228320.49068 57.27% 863564
680743.0000 771778.65839 91035.65839 -13.37% 1712739
848736.0000 972257.70807 123521.70807 -14.55% 2899014
1171377.0000 1172736.75776 1359.75776 -0.12% 4623172.5
1530495.0000 1373215.80745 -157279.19255 10.28% 7295040

Err:509

Y n (X.Y)
4831151.0000 6 17425597.0000

SE.Coefficient Mean X =
69391.17153718 Mean Y =
132116.8544943 Mean X.Y =
Mean Regresi
Mean Residual
 Kons.Baku Pembacaan Instrumen
X.X (Y-)2 (Y-Ypred)2 X Aktual (Y)
0.25 1.5274E+012 24683656840 0.5000 64135.0000
1 1.9047E+011 44444257233 1.0000 863564.0000
2.25 25018012517 3315394981.6 1.5000 1141826.0000
4 22353289937 4387755264 2.0000 1449507.0000
6.25 3.0170E+011 9599832185.6 2.5000 1849269.0000
9 1.2807E+012 2801761336.1 3.0000 2431680.0000

Err:509 Err:509 Err:509

(X.X) (Y-Ybar)2 (Y-Ypred)2 X Y

22.7500 ### Err:509 10.5000 7799981.0000

1.7500 Coefficient SE.Coefficient

### SLOPE 863001.2000 71407.34569678
### INTERCEPT -210255.2667 139045.8288915
012 R2 0.973344
10 SEP 149359.1794
F 146.0619
Sig.F 2.69E-04
Pembacaan Instrumen Aktual - Residuals Unit
Prediksi (Ypred) Prediksi (%) Kons. massa
221245.33333 ### -244.97% g/L mg mg/mL
652745.93333 ### 24.41% mg/L g g/mL
1084246.53333 ### 5.04% g/L ng ng/mL
1515747.13333 ### -4.57% %b/v pg pg/mL
1947247.73333 ### -5.30% ppm
2378748.33333 ### 2.18% ppb
ppt
g

n
6
mg/mg sampel
g/mg sampel
ng/mg sampel
pg/mg sampel
ChromatoC
ChromatoC
Parameter Kinetika
HPLC
Pemisahan
Chromatotron
(Centrufugal-TLC) KK

Analisis Kuantitatif Analisis

atoCalc
toCalc
HPLC GC

KK TLC

Analisis Regresi

Arie BS
arie_sudarmanto@ugm.ac.id
 Regression Calculator
KROMATOGRAFI : GC

ANALIT 1 : Metanol ANALIT 2 : Etanol

BAKU
Metanol Etanol
No. Kons. Peak Kons. Peak 3000000
KURVA KALIBRAS
Baku Area Baku Area
1 0.5 201138 0.5 64135 2500000
2 1 398662 1 863564
f(x) = 863001.2x - 210255.26
3 1.5 680743 1.5 1141826
2000000
Peak Area R = 0.9733443376
4 2 848736 2 1449507
5 2.5 1171377 2.5 1849269
6 3 1530495 3 2431680 1500000
f(x) = 521881.314285714x -
R = 0.9866588484
1000000

500000

0
0 0.5 1 1.5 2 2
Konsentrasi Baku

100.00%
Residuals
0.00%
-100.00%0.00 0.50 1.00 1.50 2.00 2.

-200.00%
Unit Konsentrasi baku = g/L -300.00%

1
Standard deviation of the residuals = 45.95%

Persamaan Kurva Kalibrasi (Baku):

Analit 1 Metanol Y = 400958.0994 X +
Analit 2 Etanol Y= 863001.2 X +

Metanol Etanol
Nilai SE Nilai SE
Slope = 400958.09938 69391.17153718 863001.2 71407.3456968
Intersep = 170341.50932 132116.8544943 -210255.266667 139045.828891
R2 = 0.8930136114 0.9733443376
S = 179726.25998 149359.1793635
F = 33.38793365 146.0619246523
Sig.F = 4.46E-03 2.69E-04

Perhitungan Batas Deteksi:

SD intersep
= 323618.87993 340591.3316464
YLOD = 1141198.1491 811518.7282726

LOD = 2.4213419 1.18397749 g/L

 SAMPEL
ALIBRASI No. Peak Area Bobot Vol. FP Kons.Reg. (g/L)
Analit 1 Analit 2 (mg) (mL) Analit 1
1 1116806 187132 790 1 10 2.360507225
2 1114630 185732 790 1 10 2.355080224
2x - 210255.266666667
3 1115522 189652 790 1 10 2.357304896
3376
4 1118438 188876 790 1 10 2.364577476

314285714x - 108100.466666667
8484

5 2 2.5 3 3.5
ntrasi Baku

2.00 2.50 3.00 3.50

RERATA
SD
2 CV
102.35%

170341.509317
-210255.26667
ns.Reg. (g/L) Kadar
Analit 2 Analit 1 Analit 2
0.460471279 29.8798382967 5.8287503718
0.458849034 29.8111420817 5.8082156159
0.463391322 29.8393024787 5.8657129325
0.462492134 29.931360458 5.8543308106

= 29.865410829 5.8392524327 mg/g

= 0.0522310517 0.025826942 sampel
= 0.17% 0.44%
 KROMATOGRAFI GAS
ANALIT 1 : metanol ANALIT 2 : etanol

PARAMETER KINETIK PEMISAHAN

No. Data tM tR.1 W1 tR.2 W2 k'1 k'2
(min) (min) (min) (min) (min)
1 Etanol 2.60 5.15 1.17 0.98
2 Metanol 2.60 4.30 1.21 0.65
3 Standard 0,5 2.60 4.27 0.46 5.06 0.70 0.64 0.95 1.47
4 Standard 1 2.60 4.27 0.77 5.06 0.67 0.64 0.95 1.48
5 Standard 1,5 2.60 4.28 0.75 5.07 0.76 0.65 0.95 1.47
6 Standard 2 2.60 4.28 0.76 5.07 0.94 0.65 0.95 1.47
7 Standard 2,5 2.60 4.28 0.77 5.08 0.84 0.65 0.95 1.47
8 Standard 3 2.60 4.28 0.77 5.09 0.96 0.65 0.96 1.48
9 Sampel 1 2.60 4.24 0.46 5.01 0.24 0.63 0.93 1.47
10 Sampel 2 2.60 4.24 0.46 5.01 0.25 0.63 0.93 1.47
11 Sampel 3 2.60 4.24 0.46 5.01 0.24 0.63 0.93 1.47
12 Sampel 4 2.60 4.24 0.47 5.01 0.24 0.63 0.93 1.47

RERATA = 0.64 0.94 1.47

SD = 0.0086 0.0170 0.0031
CV = 1.35% 1.80% 0.21%
 KROMATOGRAFI GAS
Kondisi Operasi Instrumen:
KOLOM FASE DIAM
Jenis Kolom : Fused Silica Capilary Jenis Fase Diam : Stabilwax

Panjang Kolom : 30 m Ukuran partikel : 0.25 m

Diameter Kolom : 0.25 0.25 Chemical bonded : Ya
inner outer

FASE GERAK
Gas pembawa : Helium Make up gas : kPa
Tekanan gas : 80.4 Make up flow : 30 kPa

SAMPEL Volume Injeksi : 0.5 L

DETEKTOR : FID Temperatur Oven : Isotermis 40 oC

Temperatur Detektor : 250 oC
Temperatur Injektor : 150 oC

Attenuator : Input range :

RECORDER Kecepatan kertas recorder : cm/menit

Skala sinyal recorder :

KELAS : C IV Yogyakarta, 12 April 2017

KELOMPOK : A
ANGGOTA : Nama Mahasiswa NIM
1 Eki Noor Istiqomah 9702
###
2 Rizki Lia Okta 10133
###
3 Niken Laras S 10136
###
4 Levina 10139
 Rs Neff.1 Neff.2

76.46
31.41
1.36 209.31 200.29
1.11 76.07 214.76
1.04 80.07 168.42
0.94 78.82 111.12
0.99 76.26 138.03
0.93 77.44 107.16
2.20 201.37 1637.87
2.17 205.80 1460.94
2.20 205.80 1585.49
2.17 197.93 1572.47

1.51 130.93 661.18

0.5928 71.3240
39.23% 54.47% #VALUE!
 C
o

C
o

C
o

C
o

12 April 2017
 KROMATOGRAFI CAIR KINERJA TINGGI
PARAMETER KINETIK PEMISAHAN

ANALIT 1 : Parasetamol ANALIT 2 : Kafein

No. Kode tM tR.1 W1 tR.2 W2 k'1 k'2

Kromatogram (min) (min) (min) (min) (min)
1 Parasetamol 1.375 1.867 0.975 0.36
2 Kafein 1.375 3.783 1.344 1.75
3 Campuran 1 1.375 1.942 0.997 3.992 1.255 0.41 1.90 4.62
4 Campuran 2 1.375 1.858 0.878 3.925 1.131 0.35 1.85 5.28
5 Campuran 3 1.375 1.858 0.815 3.950 1.221 0.35 1.87 5.33
6 Campuran 4 1.375 1.942 0.997 3.992 1.255 0.41 1.90 4.62
7 Sampel 1 1.383 1.858 0.995 3.917 1.140 0.34 1.83 5.33
8 Sampel 2 1.383 1.858 0.947 3.925 1.123 0.34 1.84 5.35
9 Sampel 3 1.383 1.858 0.999 3.917 1.184 0.34 1.83 5.33
10 Sampel 4 1.383 1.867 0.958 3.917 1.189 0.35 1.83 5.24

RERATA = 0.363 1.847 5.137

SD = 0.028 0.046 0.324
CV = 7.85% 2.49% 6.31%
 KROMATOGRAFI CAIR KINERJA TINGGI
Kondisi Operasi Instrumen:

KOLOM FASE DIAM

Jenis Kolom : packing Jenis Fase Diam : C-18
Panjang Kolom : 12.5 cm Ukuran partikel : m
Diameter Kolom : mm Chemical bonded :
inner outer

FASE GERAK
Tipe Elusi : Isokratik Eluen : Metanol-Aquadest (3:7)
Kecepatan alir : 1 mL/menit

SAMPEL Volume Injeksi : 20 L

DETEKTOR Panjang gelombang : 273 nm

Attenuator : Input range :

RECORDER Kecepatan kertas recorder : cm/menit

Skala sinyal recorder :

KELAS : C Yogyakarta, 12 April 2017

KELOMPOK : 1
ANGGOTA : Nama NIM
1 Eki Noor Istiqomah 9702
###
2 Rizki Lia Okta 10133
###
3 Niken Laras S 10136
###
4 Levina 10139
GGI

Rs Neff.1 Neff.2

4.07
51.37
1.82 5.18 69.60
2.06 4.85 81.35
2.06 5.63 71.16
1.82 5.18 69.60
1.93 3.65 79.05
2.00 4.03 82.02
1.89 3.62 73.35
1.91 4.09 72.71

1.935 4.48 72.25

0.094 0.74 9.20
4.88% 16.53% 12.74%
GGI

12 April 2017
 KROMATOGRAFI KOLOM
ANALIT 1 : ANALIT 2 :
Persamaan Kurva Baku : n R2 S
Y= ### X + ### 6 0.8930 ###
Persamaan Kurva Baku : n R2
S
Y= ### X + ### 6 0.9733 ###

Unit Konsentrasi Baku : g/L SAMPEL

Volume aplikasi sampel : mL Penimbangan Sampel :
Volume fraksi : mL Volume Sampel :
No. Absorbansi FP Kons.Reg. Konsentrasi
Fraksi Analit 1 Analit 2 Analit 1 Analit 2 Analit 1 Analit 2

ND = tidak terdeteksi Massa total Analit =

Konsentrasi total =
KADAR
Analit 1 =
Analit 2 =
M KROMATOGRAFI
Kondisi Operasi Instrumen:
F SDblank KOLOM
33.388 323618.8799 Panjang : cm FASE DIAM :
F SDblank Diameter : cm FASE GERAK :
146.062 340591.3316
DETEKTOR : UV/Vis Panjang Gelombang :

mg
mL Profil Kromato
Massa (mg) 12.0000
Analit 1 Analit 2
10.0000

8.0000

6.0000
Konsentrasi
4.0000

2.0000

0.0000
0 5 10 15

No.F

PERHITUNGAN PARAMETER KINETIKA

ANALIT
1

Volume Mati (VM) :

No.Fraksi dg kons. maks :
Volume Retensi (VR) :
Jumlah fraksi : 0
Lebar puncak (W) :

Nef :
k' :
:
Rs :

KELAS :
KELOMPOK :
ANGGOTA : Nama
0.000 0.000 mg
mg/mL

mg/mg sampel
mg/mg sampel
ROMATOGRAFI KOLOM

1 : nm 2 : nm

Profil Kromatogram

10 15 20 25 30 35 40

No.Fraksi

Column I Column J

ANALIT
2

mL

mL
0
mL

Yogyakarta, 12 April 2017

NIM
 KROMATOGRAFI LAPIS TIPIS
ANALIT 1 : PARASETAMOL ANALIT 2 : KAFEIN
FASE DIAM / PLATE : SILIKA F366 Dimensi : 10 x 20 cm
FASE GERAK / ELUEN : KLOROFORM DAN ETHANOL
DETEKTOR : Densitometer 1 : 252 nm 2 : 275 nm
Konsentrasi Larutan induk 1 1 2 1 g/L Panjang Elusi (dM) : 80 mm

DATA PARAMETER KINETIKA PEMISA

Vol.
Spot Keterangan Kons. (g/L) Massa (g) dR.1 W1 dR.2 W2 hRf1 hRf2 k'1 k'2
Totol
(L) 1 2 1 2 (mm) (mm) (mm) (mm)
1 Std. Murni Analit 1 6.00 1.00 17.5 3.2 21.88
2 Std. Murni Analit 2 4.00 1.00 45.5 3 56.88
3 Std. Campuran 4 0.2 0.02 0.8 0.08 16 2 46.5 1 20.00 58.13 0.800 0.419 0.523
4 Std. Campuran 4 0.4 0.04 1.6 0.16 17 3 45 1.5 21.25 56.25 0.788 0.438 0.556
5 Std. Campuran 4 0.6 0.06 2.4 0.24 17 2.7 45 2.2 21.25 56.25 0.788 0.438 0.556
6 Std. Campuran 4 0.8 0.08 3.2 0.32 17 3 44.5 3 21.25 55.63 0.788 0.444 0.563
7 Std. Campuran
8 Std. Campuran
9 Sampel 1 4 0.59013 0.11512 2.36 0.46 18 3.5 45 3.1 22.50 56.25 0.775 0.438 0.565
10 Sampel 2 4 0.58877 0.11471 2.36 0.46 17 2.8 46 3 21.25 57.50 0.788 0.425 0.540
11 Sampel 3 4 0.58933 0.11585 2.36 0.46 17 2.5 46 2.8 21.25 57.50 0.788 0.425 0.540
12 Sampel 4 2.36 0.46

Kons.Rerata 0.5894 0.1152 56.80 RERATA 21.33 56.80 0.79 0.43 0.55
Kons.SD 0.00 0.00 0.85 SD 0.72 0.85 0.01 0.01 0.02
Kons.CV 0.1% 0.5% 1.49% CV 3.38% 1.49% 0.92% 2.12% 2.75%

KELAS : C Yogyakarta, 12 April 2017

KELOMPOK: 1
ANGGOTA : Nama NIM
1 EkI Noor 5555
KINETIKA PEMISAHAN

Rs Neff.1 Neff.2

20.333 16384.00 17956.000

12.444 7056.00 8711.111
11.429 8711.11 4049.587
9.167 7056.00 2240.444

8.182 5020.73 2039.542

10.000 8100.00 2055.111
10.943 10160.64 2359.184

11.79 8926.93 5630.14

4.03 3654.21 5941.10
34.18% 40.93% 105.52%