Multiplex Paper Biochip

for Acute Kidney Injury Group 31

Presented
Diagnostics by Anthony
Han
Overview of Proposed Device

Multiplex diagnostic chip

Gold nanorods on paper substrate
Localized surface plasmon resonance (LSPR) shifts
Imprint artificial proteins onto chip
Biomarkers that spike quickly after AKI onset
Artificial protein molds behave like antibodies that can
bind in the presence of the protein in the future
Test with urine sample
Alternatives to creatinine

NGAL Kim-1 FABP1

Changes to Project
Initial Design Revised Design
Chip with wells separated Hydrophobic barriers were
solely by geometry added between wells,
ensuring sample separation
and increasing potential
flexibility in well shape
 Verification and
Validation of Design
Metrics
Multiplex

Verify: no interference from other biomarkers

Validate: color coded wax barriers
Biochip Output

Verify: Use LSPR imaging to

confirm red-shift
Validate: End user reads chips
with LSPR technology
Selectivity

Rejection of nonspecific biomarkers

Verify: Interfere with 20ug/mL Hb
Shift <2 nm
Validate: Interfere with artificial urine
Shift <2 nm
Sensitivity

At least 2 nm shift at a maximum of 75 ng/mL

Verify: Compare chip soaked in 75 ng/mL of each
biomarker versus control chip soaked in PB8 buffer
Validate: Chip is sensitive enough so user can discern
presence of proteins
Testing & Fabrication Time

Testing Fabrication
<24 hours <48 hours
Verify: <24 hours from Verify: Add up time
sample collection to taken for all steps
LSPR image showing Validate: Grad student in
shifts nanotech lab can
Validate: Client thinks produce chip in <48
time from collection to hours
shifts is satisfactory
Other metrics
Substrate Material
Verify: LSPR imaging to confirm
nanorod adherence to paper
substrate
Validate: Paper can be used as
substrate
Cost Storage Temperature
Verify: <$50/chip Verify: Maintains functionality 1
week after production
Validate: Client approves cost of
chip Validate: Client can easily store
chip in room temperature
environment
Proof of Concept
Testing
CRAIC Imaging of Paper Substrate

Nanorods seeded on Whatman No. 1 Filter Paper

Previously only glass had been used
Characteristic curves present after imaging
Hydrophobic Barriers

Wax barriers to separate proteins during synthesis

Hydrophobic Barriers

Wax barriers to separate proteins during synthesis

Hemoglobin Testing

Intentionally imprinted chip with Hb rather than other

biomarkers
Statistically significant shifts in recent tests

620-660 nm

2-Sample T Test Substrate A Substrate B

Significant (alpha<5%) yes yes

P-value 4.66E-04 2.88E-05

Confidence Intervals [-6.6528,-2.2312] [-9.1989,-4.1461]

Current Status & Next Steps

Test chips with Make rods and

NGAL, Kim-1, imprint proteins
FABP1 on multiplex chip
Questions?