: 10008

: , 1-, 2-, -
-.
, . 1-
1-, 1- 1- . 2-
2-, . -
1- , ,
2-
. O -
,,D,E .
2 - .

.

Gel
Tris Barbital buffer
(Amidoblack)
(staining sol.diluent)
(Destaining Solution)
7 (.1)


10l (.2)
(.3)

1. 2

3.


1. .
2. 120l .
3. . (.4)
buffer
( ). (.5, .6)

4.

5.
 6.

4.
( ) (.7,
.8)

7. 8.
5. 10l
( 2min). (.9)

9.

6. 5 .
7.
:
8. 40sec ,
, . (.10)
 10.

9. , ( ) 22min 90V-
(123mA). (.11, .12)
 11
 12.

10. fixative solution 15min.

( 80C) 35min (.13),
4min. (. 14, .15)

13.
 14. 15.

11. 2
10min. , .
(.16, .17, .18)

16.
 17.

18.
12. Phoresis
. (.19, . 20, . 21)

19.
 20.
 21.