Top Five Antibody Sequencing Service Providers in Google

DNA sequencing technology has been widely used in various fields of

biological research. Many biological problems can be solved by high-
throughput DNA sequencing technology. Over the past three years, the
massively parallel DNA sequencing platform has evolved into mainstream
sequencing technology. The sequencing technique has not only reduced 99%
of the sequencing cost, but also makes this technology, a privilege granted to
large sequencing centers previously, sharable by many researchers.

Currently, new sequencing technologies and approaches are still emerging,

such as the latest progresses, including sequence database establishment,
new sequence data analysis methods establishment and sequencing test
design. Antibody sequencing is one of the fields that catch most of the
attention in sequencing industry. Under these circumstances, biotech
companies are launching their own techniques of antibody sequencing. Lets
see the top three antibody sequencing service providers in Google Search
Engine.

Top 1. ISO9001 Certified Antibody SequencingGenScript

ISO9001 certified antibody sequencing service speeds up and facilitates

therapeutic antibody leads discovery and development. Their expertise helps
researchers avoid common pitfalls when sequencing antibodies, including
impurity of hybridoma cells, mutations introduced by PCR, and inaccurate or
misaligned full sequences.

How to Avoid Impurity of hybridoma cells

Hybridomas may contain pseudogenes and mRNAs encoding non-functional

antibodies, which may be amplified by primer pairs intended to clone the
target antibody. GenScript will sequence five independent clones for each
chain to ensure accuracy.

How to Avoid Mutations introduced by PCR

During the amplification of the cDNA, the use of degenerate primer sets
means there is the chance of mutagenesis. GenScript uses both 5'- and 3'-
RACE (rapid amplification of cDNA ends) to ensure accuracy of amplified
products.

How to Avoid Inaccurate or Misaligned Full Sequence

Once the individual sequences are obtained for each chain, they must be
aligned properly to ensure the full sequence is accurate. GenScript has
sequenced over 1,000 mAbs, their expertise ensures the sequence you
receive is 100% accurate.

Top 2. De Novo Antibody SequencingCreative Biolabs

Creative Biolabs developed a series of unique de novo antibody sequencing

services for research, diagnostic, and therapeutic industries. To overcome the
current drawbacks of sequencing, Creative Biolabs has developed the
Database Assisted Shotgun Sequencing (DASS) technology based on its next
generation antibody sequencing platform.
Its world-leading DASS system provides reliable supporting proof for the
confirmation and validation of produced mAb, QC analysis and IND/NDA
application. It can use a high-field Orbitrap Elite hybrid MS (Thermo Scientific)
with 240.000 resolution and <1 ppm mass accuracy to perform our services.
The available fragmentation techniques will include CID, HCD, and ETD. The
combined usage of these technologies could ensure the maximum coverage
to generate high-quality results for researchers.

Sequencing Monoclonal Antibodies

Purified monoclonal antibodies in multivalent forms can be sequenced with

100% coverage of the desired regions as well as excellent accuracy.

Top 3. Antibody cDNA SequencingInnovagen

There may be several reasons for wanting to obtain the variable region cDNA
of your monoclonal antibody. For example, as a means of immortalising the
cell line in-silico or when you are looking to develop your antibody further
through the process of antibody engineering. Innovagens antibody cDNA
sequencing service can be used as a stand-alone service, but it could also
form an integrated part in a larger project as it can be your partner from
antigen design and production all the way to humanised antibodies.

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Sequencing-Service-Providers-in-Google.html

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