Quang ph t ngoi kh kin

Model: U 2900
(UV-VIS Spectrophotometer)
Hng Hitachi Nht Bn

HNG DN S DNG MY QUANG PH T NGOI KH

KIN MODE UV- 2900 HNG HITACHI - NHT BN
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A. Mt s ch quan trong:
Yu cu v ni lp t: Nhit t 15- 350C, m t 45- 80%, t bi , khng b rung,
khng b nh sng chiu trc tip, khng c thnh phn kh n mn hay kh hp th t ngoi,khng
b nh hng ca t trng mnh.
Yu cu v in p: in p thay i khng qu 10%, cn qua n p, v c dy ni t
Nguyn tc chung truy nhp vo menu: Menu ca chng trnh c t chc kiu nhnh
cy, mun vo menu con ta ch vic bm vo phm ch s ng vi th t v tr ca n trn danh
sch ca s hin ti, mun quay li ca s trc ch vic bm o phm RETURN. Sau khi thay
i hay la chn mt thng s no cn bm phm ENTER xc nhn.

B. Chc nng cc phm trn mt my:

START/STOP: o v thu kt qu cng nh khi ang o
AUTO ZERO: zero kt qu
GOTO WL : nhp bc sng cn o
ENTER: xc nhn kh thay i hoc la chn thng s khc.
Cc phm mi tn tri/ phi: Di chuyn cc phm tng gim cc gi tr
RETURN: tr li menu trc hoc hu b mt la chn
MAINMENU: Tr v menu u tin
PRINT: in cc thng s hin th trn my
CLEAR: Hu b lnh va nhp
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C, Trnh t vn hnh:
Khi bt cng ngun my chnh mn hnh x hin ra :

My s t ng kim tra cc thng s : RAM, ROM, Wavelength Driver, D2 lamp, WI lamp v

bc sng 656,1nm. Tt c cc thng s trn OK th mn hnh s xut hin ( Nu khng cc
thhong s trn khng qua ccn lin h ngay vi nh cung cp):

Cc ch do:
- 1. Photometry: phng php o im
- 2. Wavelength scan: phng php qut ph
- 3. Time scan: phng php o ng hc
- 4. Method: Chn phng php lu sn
- 5. Instrument setup: t cc thng s cho my
- 6. File manager: la chn file data lu vo my
Tin hnh o mu:
- Chun b mu
- Lp phng phpo
1. Phng php o im ( Mc 3.4 Instruction manual)
- Trn mn bn phm ca my nhn Photometry (1) v ENTER mn hnh xut hin:

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- Chn parameter setup(1) v ENTER v mn hnh s xut hin:

Data mode la chn 1 trong 4 phng phn o

(1 ) T%: o theo truyn qua
(2) ABS: o theo hp th
(3) CONC: o theo nng mu
(4) RATIO: o theo t l

Initial Delay(s) Thi gian trc khi o mu thng chon l 0

Number of WL La chn s bc sng khi o
La chn theo bng sau:
Data mode khong chn
T%, ABS T 1 n 6
CONC T 1 n 3
RATIO T 2 n 3
WL t 1 n 6 C th chn bc sng t 190nm n 1100nm v
(nm) mi gi tr tng l 0,1nm

Sau nhn vo tr v mn hnh phng php o im

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- Chn Sample setup (2) v ENTER v mn hnh s xut hin:

Name t tn mu cn o
Unit label n v tnh
Sample ID S mu cn o

Sau nhn vo tr v mn hnh phng php o im

- Chn curve setup (3) v ENTER v mn hnh s xut hin

Sau nhn vo tr v mn hnh phng php o im

- Chn system setup (6) v ENTER v mn hnh s xut hin

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 Response S dng cc ch ly tn hiu
1) Fast: Ly tin hiu nhanh
2)Medium: Ly tin hiu trung bnh
3) Slow: Ly tin hiu chm
Lamp La chn n phn tch
change 1) Auto: my t ng chn n
mode 2) D2 lamp: ch mt n D2
3) WI lamp: ch n WI
D2 lamp chn bt n hoc tt n D2
WI lamp chn bt n hoc tt n WI
Path length chiu dy cuvt , cuvt chun thng l 10mm
(mm)

Sau nhn vo tr v mn hnh phng php o im.

Lp phng o xong ta nhn vo my t xc nhn l t xong phng php mn hnh

xut hin:

- Cho mu trng vo my v nhn a gi tr o v 0

- Cho mu cn o v nhn bt u o mu mn hnh xut hin:

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Sau o nhn vo kt thc qu trnh o mn hnh xut hin:

1)Measure Nhn vo 1 tr v phng php o

2)Continue Nhn vo 2 tip tc o muc tip theo
3)Save Nhn vo 3 lu li
4) Remesure Nhn vo 4 i tn phng php
5) Print setup Nhn vo 5 in ph v kt qu o, nu
ni trc tip vi my in.

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Sau nhn vo tr v mn hnh phng php o im v tr v mn hnh ban u

2. Phng php qut ph ( Mc 3.5 Instruction manual)

- Trn mn hnh bn phm nhn vo 2 (Wavelength scan) v ENTER mn hnh xut hin:

Chn parameter setup (1) v mn hnh xut hin:

Data mode La chn 1 trong 4 thng s

1) T% : Qut theo truyn qua
2) ABS: Qut theo hp th
3) E(S) : Nng lng mu
4) E(R): Nng lng mu so snh
Start WL(nm) Bc sng bt u c th chn t 190
n 1100nm
Stop WL (nm) Bc sng kt thc c th chn t 190
n 1100nm
Upper scale Gi tr trn trc OY
Lower scale Gi tr trn trc OX
Scan speed (nm/min) Tc qut:10;
100;200;400;800;1200;2400;3600
Initial delay (s) thi gian ch trc khi qut thng l 0

Sau nhn vo tr v mn hnh phng php qut ph

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- Chn Sample setup (2) v nhn ENTER mn hnh xut hin:

Name: t tn phng php

Sau nhn vo tr v mn hnh phng php qut ph

- Chn Systum setup (4) v nhn ENTER mn hnh xut hin:

Baseline S dng cc ch qut

1) System: Qut ton gii t 190 n
1100nm
2)User 1: Qut theo ch ngi s dng
3) User 2: Qut theo ch ngi s dng
Response S dng cc ch ly tn hiu
1) Fast: Ly tin hiu nhanh
2)Medium: Ly tin hiu trung bnh
3) Slow: Ly tin hiu chm
Lamp change La chn n phn tch
mode 1) Auto: my t ng chn n
2) D2 lamp: ch mt n D2

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 3) WI lamp: ch n WI
Lamp change Bc sng chuyn n nm trong khong
WL(nm) 325 ns 370nm thng t l 340nm
D2 lamp chn bt n hoc tt n D2
WI lamp chn bt n hoc tt n WI
Path length (mm) chiu dy cuvt , cuvt chun thng l
10mm

Sau nhn vo tr v mn hnh phng php qut ph v nhn vo xc nh

l lp xong phng php.
- Cho mu trng vo v nhn vo Baseline cc gi tr v 0 mn hnh xut hin:

- La chn Baseline System hoc User 1 hoc User 2 v nhn ENTER. Sau khi Baseline

xong ta em mu vo tin hnh o. Nhn vo bt u o mu, my s t ng

qut ph theo nh phng php ta t t trc v cho ta data file nh hnh v:

Sau khi o xong ta lu li cc data va o xong v ghi li kt qu

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- Nhn vo tr v mn hnh phng php qut ph v v mn hnh ban u

3. Phng php qut ng hc ( Mc 3.6 Instruction manual)

- Trn mn hnh bn phm nhn vo 3 (Time scan) v ENTER mn hnh xut hin:

Chn parameter setup (1) v mn hnh xut hin:

Data mode La chn 1 trong 4 thng s

1)T% : Qut theo truyn qua
2)ABS: Qut theo hp th
3)E(S) : Nng lng mu
4)E(R): Nng lng mu so snh
WL(nm) Nhp bc sng cn qut nm trong
khong 190 n 1100nm
Upper scale Gi tr trn trc OY
Lower scale Gi tr trn trc OX
Scan speed (nm/min) Tc qut:10;
100;200;400;800;1200;2400;3600
Initial delay (s) thi gian ch trc khi qut thng l 0

Sau nhn vo tr v mn hnh phng php qut ph

- Chn Sample setup (2) v nhn ENTER mn hnh xut hin:

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Name: t tn phng php

Sau nhn vo tr v mn hnh phng php qut ph

- Chn Systum setup (4) v nhn ENTER mn hnh xut hin:

Response S dng cc ch ly tn hiu

1) Fast: Ly tin hiu nhanh
2)Medium: Ly tin hiu trung bnh
3) Slow: Ly tin hiu chm
Lamp Bc sng chuyn n nm trong khong 325
change ns 370nm thng t l 340nm
WL(nm)
Lamp La chn n phn tch
change 1)Auto: my t ng chn n
mode 2)D2 lamp: ch mt n D2

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 3)WI lamp: ch n WI
D2 lamp chn bt n hoc tt n D2
WI lamp chn bt n hoc tt n WI
Path length chiu dy cuvt , cuvt chun thng l 10mm
(mm)

Sau nhn vo tr v mn hnh phng php qut ng hc v nhn vo xc

nh l lp xong phng php.

- Cho mu trng vo v nhn gi tr v 0 v a mu cn o vo ri nhn my s

t ng qut xong roi mn hnh xut hin:

Sau lu li data va o xong v ghi li kt qu, Nhn vo tr v mn hnh phng

php qut ng hc v mn hnh ban u:

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4. La chn phng php ( Mc 3.7 Instruction manual)
- Sau khi t phng php xong ta co th goi phng php ra s dng cho cc ln sau vi cc
mu ny lm cho cc ngy khc nhau m khng cn lp li na.
5. Ci t cc thng s cho my ( Mc 3.8 Instruction manual)
Nhn vo 5 (Instrument setup) v ENTER mn hnh xut hin:

WL cabration Hiu chun li bc sng

0% T Hiu chun truyn qua
Lamp time Xem gi n
GLP/GMP(Auto) Kim tra my t ng
GLP/GMP(manual) Kim tra my bng tay
Cc thng s khc moi ngi c th tham kho trong sch Instruction manual s chi tit
6. Tt my:
- Dng phm RETURN a mn hnh v my mn hnh ban u

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 - Tt cng tc ngun ca my.
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Mi thc mc xin lin h : 04-37912699 (s l 204) hoc 0945963881 Thanks!

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