7. Study the effects of temperature on salivary amylase activity.

Problem statement: What are the effects of temperature on the activity of salivary amylase?
Hypothesis: When the temperature increases, the rate of enzyme reaction increases.
Variables:
Manipulated variable: Temperature of the reaction
Responding variable: The rate of enzyme reaction
Control variable: Volume of enzyme
Apparatus and material: Beakers, tile with grooves, test tube, thermometer, syringe,
stopwatch, Bunsen burner, tripod stand, wire gauze, glass rod, starch suspension, saliva
solution, water bath, ice cube and iodine solution.
Procedure:
1. Use a syringe to put 1 ml of 0.1 % starch suspension into each of test tubes labelled
A1 and B1.
2. Use all other syringe to put 2 ml of saliva into each of test tube labelled A2 and B2.
3. Immersed test tube A1 and A2 into a water bath at 0oC.
4. After 5 minutes of immersion for the starch suspension from test tube A1 into test
tube A2. Stir the mixture using a glass rod and start the stopwatch.
5. Use a dropper to put a drop of mixture from test tube A2 onto the iodine solution in
the first groove of the white tile.
6. Repeat the iodine test every minute for 10 minutes.
7. Rinse the dropper after each sampling.
8. Record the time taken for the hydrolysis of starch using stopwatch.
9. Keep the test tube with the mixture in their respective water bath throughout the
experiment.
10. Repeat the experiment with different of water bath at 20C, 37C, 45C and 60C.
11. Repeat the experiment to get average readings.
12. Record the result in the table.
Presentation of Data:
Temperature/C Time taken for complete hydrolysis Rate of reaction (1/min)
of starch (minutes)
0
20
37
45
60