Biochemistry RBCs Team

10
() :

()

:

O2 a
O2 b

:




The final common pathway of fuel metabolism
Acetyl CoA

Biochemistry RBCs Team

Matrix

:
ATPCO2

FADNAD
;

i
PDC ii

E1 a
E2 b
E3 c
A

Biochemistry RBCs Team

:
E1
CO2E1

B1 o

E2

E3
Acoenzyme AE3

Biochemistry RBCs Team

SH

FADH2FAD,

Biochemistry RBCs Team

NAD FADH2
H+NADH

V B1
NADCO A

NADH+H+CO2A

http://www.brookscole.com/chemistry_d/templates/student_resource
s/shared_resources/animations/pdc/pdc.html
^_^animation

:



A
B

Biochemistry RBCs Team

ADP,AMPNADCO A
Ca+


NADH+H+Acetyl CO AATP

ATP


lactic acidosis LDH

Pyruvate Dehydrogenase Subunits

Enzyme Abberviated Prosthetic Group

Pyruvate dehydrogenase E1 Thiamine
pyrophosphate(TPP)
Dihydrolipoyl E2 Lipoamide
Transacetylase
Dihydrolipoyl E3 FAD
Dehydrogenase

Biochemistry RBCs Team

: Acetyl co A

Acetyl Co-A

Acetyl Co-A o
citrate synthase o
Co-A o

o
cis-aconitase o
o
o

H2O H2O
Acetyl
Co-A

Acetyl Co-A o
C14 Acetyl Co-A
CO2 o


o
CO2

Biochemistry RBCs Team

CO2

CO2

NAD+


CO2

NADH

.
FADNAD+Co-A

(Reference: The coenzymes required are thiamine pyrophosphate, lipoic acid, FAD, NAD+, and
coenzyme A.)
A
NADHCO2
Co-A

Biochemistry RBCs Team

CO2
A
H+NADH

Co-A
GDP
ATP

GTPGDPCo-A
ATP
GTP + ADP GDP + ATP

Substrate-level ATP

coA-SH

:


FAD

Succinate thiokinase (also called succinyl CoA synthetase) :

ATP

Biochemistry RBCs Team

NAD+
NADH

:

3NADH



1FADH2
1ATP
12ATPAcetyl Co-A
9ATP3ATPNADH o
2ATPFAD o
1ATP o

 Biochemistry RBCs Team

ATP

ATPNADH Co-A

ATP

Biochemistry RBCs Team

39ATP
o
G6PATPG6P o

:
Acetyl Co-A , 3NAD , FAD , GDP , H2O o
2CO2 , 3NADH+H+ , Co-A , FADH2 , GTP o
Amphibolic

Acetyl Co-A

:
o

GOT
transaminase


A
A

Acetyl Co-A o

Biochemistry RBCs Team

:
o
o
o
GABA
A o
o

o

Biochemistry RBCs Team

o





ATP
Co-A

A


A

o


o

o

Biochemistry RBCs Team

o
ATP

o



o

Flash animation of krebs cycle:

http://www.1lec.com/Biochemistry/How%20the%20Krebs%20Cycle%20Works/
index.html