You are on page 1of 2

Growth in Liquid mediumDifferential

(Nutrient broth) andusing


Staining Solid Gram
medium (Nutrient
Staining agar)
(gram +/-) and Basic Fuschin (a

Simple Staining using Methylene Blue and Nigrosin (cell shape


Morphological and Cultural Characteristics

Bacteria Isolates Spore Stain (+/-)

Physiological and Biochemical Characteristics


Temperature Requirement
(5-8 oC, 45 oC, 55 oC)

Reaction on Litmus Milk Oxygen Requirement


(spectrophotometer)
Starch Hydrolysis (+/-) pH Requirement
Catalase Test (+/-) (via spectrophotomer)

Nitrate Redn Test (+/-)

Gelatin Liquefaction (+/-)

KOH Test (+/-)

You might also like