Friday, March 10, 2017 LSBIOC 370 Dr. Karen Bame Chapter 11 Chapter 12 I. © Introduction to Metabolism A. Overview B. Characteristics of Metabolic Pathways C. Control of Metabolic Pathways Il. Questions to Ask when Studying Metabolism For Monday, March 13: Chapter 12: Carbohydrate metabolism Pages 369-380 and 383-384Housekeeping Items: * Please email me (bamek@umkc.edu) if you would like to meet with me outside of class. My office is 417 BSB. Abbreviated lecture slides will be available before class, so you can use them to take notes with. | will also post scanned lecture slides after each class. You will find these items on the Course Blackboard site. Access to the Panopto recordings will be on the Course Moodle site 3 to 4-point Mastering Chemistry homework assignments are due by 8:00 AM before every lecture. The next homework assignment becomes available at 10:00 AM after that day’s lecture. Access to the Mastering Chemistry homework is through the Course Moodle site. Up to 2 poll questions during each lecture, and they may be asked at any time during the lecture. There is always the possibility of unannounced quizzes over the next 11 lectures. They may be given at the beginning, the middle or the end of the lecture. There will be NO MAKE-UP quizzes — if you are not in class for the quiz, then you will not earn quiz points.Metabolism * sum of all the enzyme-catalyzed chemical reactions found inside cells acquscim AT? Uhabeentae CO aehnpoe Nhe Aker Fry /Fau,_ * composed of two contrasting processes Fig. 11.1a 4 Cry (ret) catasousm "SYMP aNagousm ens Complex Complex * OK dchve Clesvadehve- lrosgnte he ee v Co, (ond) Simple ‘Simple Co, (a) Uke, Fad, PAPRI Comedy © 2016 Pearson Education, Inc. ¢ subdivided into three stages Fig. 11.1bvoce SSeS ove et dewplat “divers Overview of Intermediary Metabolism Fig. 11.2 — i Oualeelee process veyulale processes jnlecenmnectins ‘©2016 Pearson Education, Inc.Importance of metabolic pathways * organizing reactions into pathways allows for control of free Properties of Metabolic Pathways AG consumed/released is transferred K energy aS AG ee Oy eee Ere] omd proms Smal" wee A et Sheps ered Z \i st series of reactions that convert substrate to =. product thru a series of intermediates aa = each step in pathway is catalyzed by a distinct enzyme lemued ols A enrgme A ven : rate of the pathway is controlled by altering a activity of individual enzymes =— product \, _ " i : t aa to/from molecules such as ATP or NADH_ metabolism is connected to steady-state conditionsControl of Metabolic Pathways Need to control pathways so the cell doesn’t waste energy One or more enzymes in pathway is regulated characteristics of regulated enzymes * usually near the beginning of the pathway Woslay vesouveto * usually catalyze first step ) unique to the A { B 1 c pathway clint mouek ASF 4 Ed ves, Shep a E | * usually catalyze a thermodynamically F irreversible reaction t J & c bre * AGO -Zo bdlut On dwechure® “1 Bid on [oFF "rate-determining step” of the pathway ose. D> Uwe ye Ee a produck KGS no okey BE Aton te lee Cee’ to Patkucy preductAnabolic and catabolic pathways are distinct * an anabolic pathway is not simply the reverse of a catabolic pathway b hey Fr bos ak ae ————__ 5 Me Envy we 6 skeps ee phony * every pathway is exergonic NG pag Aweely affect oon Ns dee, \ocalnus* Hormonal Regulation Sl ual TRANSDUCTION Came Cre. erie se ae Ack. Revvolvarswie E Y bok wet ¢ Pathway Compartmentation Fig. 11.10 2olcaryere cers Noplasim Vredan dred - ins (wernents,) oe e FA i) oxidehdQuestions to ask when studying metabolic pathways ue 2: What is the overall goal or purpose of the pathway? ls it an anabolic or catabolic pathway? What does this mean in terms of energy? Is ATP used or produced? Is NADH used or produced? Where does the pathway occur inside the cell? Where does the pathway occur in the body? What are the principal substrates and products of the pathway? Are there other possible substrates? Do intermediates of the pathway participate in other pathways? What types of reactions occur in the pathway? What steps in the pathway are regulated? How are they regulated? Under what conditions in the cell/organ/body will the pathway be turned on or off?