concentracion

tiempo 8.13 5.7 4.9 4.1 3.25

1 5.9 5 4.5 4.2 3.8
2 11.9 10 9 8.5 7.8
3 18 15 13.3 12.6 11.5
4 24 20 17.5 16.5 14.7
5 30 24.5 22 20 18
6 36 29 26 23

tiempo concentracion (P)

0 0 0 0 0 0
1 1.87E-05 1.59E-05 1.43E-05 1.33E-05 1.21E-05
2 3.78E-05 3.17E-05 2.86E-05 2.70E-05 2.48E-05
3 5.71E-05 4.76E-05 4.22E-05 4.00E-05 3.65E-05
4 7.62E-05 6.35E-05 5.56E-05 5.24E-05 4.67E-05
5 9.52E-05 7.78E-05 6.98E-05 6.35E-05 5.71E-05
6 1.14E-04 9.21E-05 8.25E-05 7.30E-05 0.00E+00

tiempo Concentracin (S)

0 8.13E-04 5.70E-04 4.90E-04 4.10E-04 3.25E-04
1 7.94E-04 5.54E-04 4.76E-04 3.97E-04 3.13E-04
2 7.75E-04 5.38E-04 4.61E-04 3.83E-04 3.00E-04
3 7.56E-04 5.22E-04 4.48E-04 3.70E-04 2.88E-04
4 7.37E-04 5.07E-04 4.34E-04 3.58E-04 2.78E-04
5 7.18E-04 4.92E-04 4.20E-04 3.47E-04 2.68E-04
6 6.99E-04 4.78E-04 4.07E-04 3.37E-04 3.25E-04

1/[S0] 1230.01 1754.39 2040.82 2439.02 3076.92

1/[V0] 53389.83 63000.00 70000.00 75000.00 82894.74

velocidad vs sustrato
120000.00
100000.00
f(x) = 17.3642473551x + 32513.3789627468
80000.00 R = 0.9903128539 velocidad vs sustrato
Linear (velocidad vs sustrato)
60000.00
40000.00
20000.00
0.00
1000.00 2000.00 3000.00 4000.00 5000.00
 60000.00
40000.00
20000.00
0.00
1000.00 2000.00 3000.00 4000.00 5000.00

inhibidor competitivo NO porque vara la ordenada

inhibidor a competitivo NO porque varia la pendiente
inhibidor no competitivo SI porque 1/Km se mantiene casi cte.
 2.44
3
6.1
9.2
11.8
14.2

A= *b*c
0 c(p)=A/(b*)
9.52E-06
1.94E-05 = 3150 M-1cm-1
2.92E-05 b= 1cm
3.75E-05
4.51E-05
0.00E+00

c(S)=c0(S)-c(p) tiempo velocidad de hidrlisis de sus

2.44E-04 0 - -
2.34E-04 1 1.87E-05 1.59E-05
2.25E-04 2 1.90E-05 1.59E-05
2.15E-04 3 1.94E-05 1.59E-05
2.07E-04 4 1.90E-05 1.59E-05
1.99E-04 5 1.90E-05 1.43E-05
2.44E-04 6 1.90E-05 1.43E-05

4098.36 1/[S0] 4000.00 2000.00

105000.00 1/[V0] 285714.29 181818.18

Vmax 3.08E-05
velocidad vs
350000.00
Km 5.34E-04
1/Km 1.87E+03 300000.00
250000.00 f(x) = 50.96913037
vs sustrato 200000.00 R = 0.9997562683
ocidad vs sustrato) 150000.00
100000.00
50000.00
0.00
0.00 1000.00 2000.00 3000.0
150000.00
100000.00
50000.00
0.00
0.00 1000.00 2000.00 3000.0
cidad de hidrlisis de sustrato= c(n-1)-c(n) [M/min]
- - - -
1.43E-05 1.33E-05 1.21E-05 9.52E-06
1.43E-05 1.37E-05 1.27E-05 9.84E-06
1.37E-05 1.30E-05 1.17E-05 9.84E-06
1.33E-05 1.24E-05 1.02E-05 8.25E-06
1.43E-05 1.11E-05 1.05E-05 7.62E-06
1.27E-05 9.52E-06 -5.71E-05 -4.51E-05

1333.33 1000.00
149253.73 133333.33

V'max 1.23E-05
K'm 6.26E-04
velocidad vs [S] con inhibidor Kl 3.33E-03
1/km 1.60E+03

f(x) = 50.9691303721x + 81344.1947770306

velocidad vs [S] con inhibidor
R = 0.9997562683
Linear (velocidad vs [S] con
inhibidor)

1000.00 2000.00 3000.00 4000.00 5000.00

 inhibidor)

1000.00 2000.00 3000.00 4000.00 5000.00

 v0 (s-1)
Concentracion Glucosa (mM)
A B C
5.00E+00 0.066 0.044 0.04
1.00E+01 0.11 0.078 0.07
2.00E+01 0.165 0.126 0.115
4.00E+01 0.22 0.183 0.17
8.00E+01 0.26 0.235 0.225 3.00E+01

1/[Gluc] A B C 2.50E+01
2.00E-01 1.52E+01 2.27E+01 2.50E+01 f(x) = 109.552
R
f(x)==0.999841
98.5297
1.00E-01 9.09E+00 1.28E+01 1.43E+01 2.00E+01
R = 0.999990
5.00E-02 6.06E+00 7.94E+00 8.70E+00
2.50E-02 4.55E+00 5.46E+00 5.88E+00 1.50E+01
1.25E-02 3.85E+00 4.26E+00 4.44E+00 f(x) = 60.4431
R = 0.999975
1.00E+01

5.00E+00

0.00E+00
0.00E+00 5.00E-02
3.00E+01

2.50E+01
f(x) = 109.5520739073x + 3.1713470412
R
f(x)==0.9998414633
98.5297844609x + 3.0047604058
2.00E+01 A
R = 0.99999088
Linear (A)
1.50E+01 B
f(x) = 60.4431410883x + 3.0545843046
Linear (B)
R = 0.9999752815
1.00E+01 C
Linear (C)
5.00E+00

0.00E+00
0.00E+00 5.00E-02 1.00E-01 1.50E-01 2.00E-01 2.50E-01
Inhibidor competitivo respecto de la glucosa ya que comparten la misma ordenada
Vmax 0.32743942
Km 0.01964637
K'mB 0.03208906
KlB 0.12473684
K'mC 0.0356909
KlC 0.13836735
 v0 (s-1)
Concentracion Oxigeno (M)
A B
25.00
7.50E-02 0.068 0.05
1.00E-01 0.08 0.056
1.35E-01 0.094 0.062 20.00
f(x) = 0.6825340
1.50E-01 0.1 0.0645 R = 0.99894722
2.00E-01 0.112 0.07 15.00
2.50E-01 0.122 0.073 f(x) = 0.7010275
R = 0.99939492
10.00
1/[O2] A B
13.33 14.71 20.00
10.00 12.50 17.86 5.00
7.41 10.64 16.13
6.67 10.00 15.50 0.00
5.00 8.93 14.29 2.00 4.00 6.00
4.00 8.20 13.70

Inhibicin del tipo acompetitivo por tener la misma pendiente

Vm 0.18497965
Km 1
(1+(I)/Kl) 2.02737699
Kl 0.10706878
 f(x) = 0.6825340686x + 10.966626486
R = 0.998947222
A
f(x) = 0.7010275899x + 5.4060999992 Linear (A)
R = 0.9993949271
B
Linear (B)

4.00 6.00 8.00 10.00 12.00 14.00