20 amino‘ieidos: fexilalanina Tencina T a P|. [em uow Ty vad | Ge van [opr 5 fo | phe vue tw vac | cs voc fol e tease: i] fie uua Stop Uaa | StopuGa Ja} | Met=metionina m| | te uva Siopuac | Tip'uad a} e | Yat~ vata «| [ie cow Hs CAU [arg cou ful | po er, a Leu CUC His cac | Arg CGO {C]T | The =treonina a] [res cua Gin caa | Ae cca faa | Ryo tren [fee cus an caa_| are coa_ fal, | 2am ° Tae aor ‘aux AAU | Ser aau ful istidina 2 Ja | ne auc ‘am aac | ser acc |c|? tana SYP | ae sua tye aaa | arg aca [alt operagion Met AUG tye aaa | aw ace [a gists val Gut asp Gau | Gly Gau [uy spartato Val ave ap aac | ay aac |e aluiamaio Val Ua Gi daa | oy aca [a seina val Gua Gh aac_| ay aac _|a Tip = wiptsfano ‘Arg = arginina indica el inicio de la traduceién Gly=alicina indica la fnalizacion de ésta Elcédigo genético es degenerative puesto que diferentes codones codifican para un mismo aminodeldo Occurrence! PKyof PK, of, pKyof lonizing Residue’ Mass in Proteins Nome Abbreviations COOH Group NHS Group Side Chain” (Galtans) drnch 96) Alanine AAla 23 ex 7108 20 Arginine Rang 22 90 156.20 47 Asparagine Nasa ss aay Asparticacil De Asp 38 11509 Cysteine Om Los 103.14 filemon’ Qa ct = wet Guumicadd — EGiu or 4 Gycine Gay = Histidine H.Bis 60 teoleucine Te Leucine Lie = Lysine Kus 10.0 Methionine M.Met = Phenylalanine Phe = Praline PPro = Serine S.Ser = Threonine LO 10 — Trypicphan Wp oa = Tyrosine Xr a 101 Valine oval 26 = “approntmate vans found for side casing on the fie amie aed, To obtain the mass of the amino acd Itself, 9M the mass of « mole of water, 18.02 g, The values given are for neural side chains lightly ciferentvakies wil apy at pH yale where protons have Reengsined ot ost rom the sk chains "average for a lage umber ef proteins. Individual poten can chow are deviation rom thee vas,‘No polares ( hidréfobos ) Alaina Ala-A CH, cH, : \ Valina-Val-v (* Cy CH cH, Loucina-Leu-L (*) cH, \ DoH cH cH, Isoleucine-ie-(*) CH — CH t CH, 4 Prajna ProP H,-° t H.C. =x Melioning Meta (*) CH. S— CH, CH, Polares, sin carga Sorina Ser-S HO — CH, Treonina-the-T (*) | H.-C OH Cisteina Oye HS — CH, wf _\- cH, Asparagina-Asn-N aN pone 0 Glutamina-Gin-@ Con carga negativa Con carga positive Arginina-Arg-R (lactantes ) NH, C- NH-CH.- CH.» CH. NH, + ising Hig @ph6.)( lactantes )terminal Preferred Site* Source Ri = Lys, Arg From digestive systems of animals, many other sources Ry = Tyr, Trp, Phe, Leu Same as trypsin Ry = Arg From blood; involved in coagulation V-8 protease From Staphylococcus aureus Prolyl endopeptidase Lamb kidney, other tissues Subtilisin Very little specificity From various bacil Carboxypeptidase A Ra = C-terminal From digestive systems amino acid of animals ‘Thermolysin Ry = Leu, Val, He, Met From Bacillus thermoproteolyticus “The residues indicated are those next to which cleavage is most likely. Note that in some cases pref erence is determined by the residue on the N-terminal side of the cleaved bond (Ry) and sometimes by the residue to the C-terminal side (R). Generally, proteases do not cleave where proline is on the other side of the bond. Even prolyl endopeptidase will not cleave if R, = Pro.