phn tch cht lng thc phm

Bi 3: . Xc nh hm lng ng kh trong hoa qu

bng phng php Betrand
1). Dng c v thit b:
- pipet; buret, phu lc, bnh tam gic, bnh nh mc 250
ml, bp in.
2). Ha cht :
-100 ml Kaliferoxianua K3[Fe(CN)6] 15%;
- 100 ml NaOH 10%.
-250 ml KMnO4 0,1N;
-HCl c;
-50 ml Zn(CH3COO)2 30%;
- Dung dch Fehling A: Cn 4 g CuSO 4.5H2O ha tan v nh mc bng nc
ct ti 100ml.
- Dung dch Fehling B: cn 20 g mui tartrat kp v 15 g NaOH vo 2 cc
khc nhau, sau em ha tan ln v ngui ri nh mc ti 100 ml .
- Dung dch Fe2(SO4)3: Cn 12,5 g Fe2(SO4)3 ha tan trong 100 - 150 ml
nc. Tip theo cho thm 10 ml H2SO4 m c ri nh mc ti 250 lt.
3. Tin hnh:
a) Chun b mu:
- Cn 10 qu. Nghin cn thn v cho vo 30 ml nc
ct nng 70 -800C ha tan mu. Lc ly nc chit.
Chuyn ton b dch chit vo bnh hnh nn
100ml.
- un cch thy 800C trong 15ph. thnh thong lc
u trong khi un. ngui n nhit phng
- Kh tp cht: Cho vo 5ml kaliferoxianua 15%, lc
u, yn trong 2-3 ph. Cho thm 5ml Zn(CH 3COO)2
30%,lc mnh.
- Tin hnh lc kt ta bng giy lc , ra kt ta bng
nc ct nng cho sch.
- Cho dung dch vo bnh nh mc 250ml, trng li
dng c cha dung dch vi ln vi nc ct. Nc trng cho c
vo bnh (khng c qu 250ml). trung ha axit hu c c
trong dch th bng dung dch NaOH 10% n khi pH=7,0
(kim tra bng giy ch th mu vn nng). Cui cng thm n-
c ct va 250ml. em i xc nh hm lng ng tng.
b) Xc nh hm lng ng;
- Dng ng ong ly 10 ml Fehling A v 10 ml Fehling B cho vo bnh
tam gic ri dng pipet ht 10 ml dung dch mu pha long trn vo. Lc
u ri t bnh tam gic ln bp in hoc bp ga, un sao cho sau 3 4 pht
th si v cho si tip ng 3 pht na (Sau khi un si, dung dch
vn phi c mu xanh bic c trng. Nu dung dch b mt
mu hon ton, mu lc, vng hoc nu chng t lng dung
dch feling cho vo khng oxi ha lng ng trong
mu. Trong trng hp ny cn lm li th nghim hoc vi lng
thuc th nhiu hn hoc vi lng mu it hn)
. Ly bnh ra khi bp v lng, sau em lc qua phu xp ri ra nhiu
ln bng nc ct nng 70 800C.
Ch : Khi lc nng phi lun gi nc trong phu trnh hin tng oxit
ng b oxy ha do tip xc vi khng kh. Ra xong ta dng 20 ml dung
dch Fe2(SO4)3 ha tan oxit ng, ri cng ra 2 3 ln bng nc nng.
Dung dch nhn c sau khi ha tan v ra em chun bng dung dch
KMnO4 0,1N n xut hin mu hng v khng mt i sau 2 3 giy.
-
c) Tnh kt qu : Hm lng ng ton phn biu th bng
ng glucoza hoc ng nghch chuyn (g) trong 100g
thc phm c tnh bng cng thc:
a V 100
X dm
1000 V xd m
X: Hm lng ng kh tnh theo %
a : S mg ng nghch chuyn hoc ng glucoza (mg)
tng ng vi s ml KMnO4 0,1N ( bng th tch ca KMnO4
tiu th th nghim mu tht tr i th tch KMnO 4 tiu
th mu trng)
Vdm : th tch sau khi nh mc,ml
Vxd : th tch mu ly xc nh hm lng ng kh
m : Lng mu cn ly phn tch
1000 : Chuyn t mg sang g