QUANTITATIVE ANALYSIS OF BICOMPONENT DYE

SOLUTIONS BY DERIVATIVE SPECTROSCOPY :

For example the absorbance (zero-order) spectra of single
and binary solutions of the red and scarlet is shown

In the case of binary mixtures of red / scarlet, due to

spectral overlap of zero-order spectra, simultaneous
determination of both colorants is not accurate by direct
absorption measurements. Therefore, simultaneous
determination of both dyes by normal method was
not done with sufficient precision.

However, the obtained results show that in case of strong

overlapping spectra the accuracy of the derivative
method is higher and it can be easily applicable for the
analysis of the dye contents.

Some possibilities that may be chosen to solve this

problem are derivative spectroscopy methods such as
zerocrossing or ratio spectra derivative techniques.

1
In the case of binary mixtures of red / scarlet, due to
spectral overlap of zero-order spectra, simultaneous
determination of both colorants is not accurate by direct
absorption measurements. Therefore, simultaneous
determination of both dyes by normal method was
not done with sufficient precision.

However, the obtained results show that in case of strong

overlapping spectra the accuracy of the derivative
method is higher and it can be easily applicable for the
analysis of the dye contents.

Some possibilities that may be chosen to solve this

problem are derivative spectroscopy methods such as
zerocrossing or ratio spectra derivative techniques.

2
Ratio first order derivative spectra of different
concentrations of scarlet (0.0025, 0.01, 0.025, and 0.05 g/l)
using 0.025 g/l red as divisor ( At 435 nm )

Ratio first order derivative spectra of different

concentrations of red (0.0025, 0.01, 0.025, and 0.05 g/l)
using 0.025 g/l scarlet as divisor ( At 540 nm )