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EFECTO DEL TIEMPO DE ALMACENAMIENTO REFRIGERADO SOBRE LA CALIDAD BACTERIOLOGICA DE LA LECHEt Effect of time of cool storage on bacteriological quality of milk Carlos Pedraza G.?, Hernén Agiero E.* y Selfa Godoy 0.* SUMMARY Three milk samples were obtained in a monthly scheme, in 10 selected upper level dary farms, from the Region Metropolitana (Chile). Milk samples were collected, at the end of the first milking, when roceivad in the tank, keeping them i flasks and afterwards held Yor 48 hr at 40 C, simulating a cooling tank; methylene biue reduction test (MBRT; fr), total bacterial (TBC), psychrotrophic (PC) and col: form (CC) counts/n! were determinad, at time 0, 24 and 48 hr, in each sample, The differences be ‘ween milk bacteriological quality variables due to storage time were analysed through ANOVA, in 3 ‘complete random pattern, “The average results were: TBC: 36,760 b, 98,983 ab, and 79,516 a (P < 0.08): PC: 2.975 ¢, 6,906 b, and 84,480 a (P < 0.05); CC: 1,073 a, 1,157 a, and 1,369 a; MBRT: 6.32 a, 632, and 6.13 a, Con- sloering different standards of bacteriological quality, milk stored for two days wae appropiate in {68.9-100; 30-733; 10-36. and 23.3-33,30/o of the samples, according to MBRT, TBC, PC and CC, respectively. Consequently, milk collection every other day would be severely restrained because of strang PC de- velopment during the last 24 hr of storage and, to @ less extent, because of TBC; although CC was numerically constant, it would also limit collection every other day, due to the high initial contamina: tion by these microorganisms. Furthermore, these results would show a remarkable overestimation of the bacteriological quality in refrigerated milk, a8 MBRT is the official test used in Chile to classify raw milk, INTRODUCCION Los registros de calidad de leche recepcionada por as plantas lecheras en los perfodos 1978/79 y 1980/81,

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